LOCUS       pICSL12002              4009 bp ds-DNA     circular     07-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     repeat_region   complement(749..760)
                     /label="AAV2 ITR (fragment)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
     Misc._feature   837..840
                     /label="GGAG 4bp overhang"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     Promoter        841..1014
                     /label="AtUBL5 promoter At5g42300"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /vntifkey=29
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     Promoter        1016..2499
                     /label="AtUBL5 promoter At5g42300"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /vntifkey=29
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     5'_UTR          2500..2595
                     /label="AtUBL5 5'UTR At5g42300"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /vntifkey=52
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     Misc._feature   2597..2600
                     /label="AATG 4bp overhang"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        2722..2826
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2817..2897)
                     /label="INT2_ECOLX (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             2985..3041
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             3048..3836
                     /label="S3AD_ECOLX"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 CGGGTTGATC AATATCTTTC CCTTGTTCTG ATCCTATGTA ACTACATCTT TTGTTCCGAG
      901 AAAATTGATT TTATAGTTTT TTTGTTTTCT TTTCTTTTTT CTTGATTTTA TAGAGTTATT
      961 ACATATATTT AAAAATAATT CTTGTTTAAT TATTTAATAA TTTTCATTTT ATATGTGAAA
     1021 TATTTTTTTA TAATTTTAAA CCAATGAATT TTTAGATATT TTTACTTTTT AATGACATTA
     1081 TGATATTTAT GTTTTTAAAA AAAAAATTAA CGATGAAAAG TTAATCCTAT AATTTATATT
     1141 GATGTTATAC TTAATTTATA CAAAAATTGA AAGGTTTAAA TAAAATCTTG TTTTTTTTTA
     1201 AAACGTCGTC GATCTGTTTT GAAAACGTTG CAATTTTTGT TAATCCAATT TATGGAAACA
     1261 TGTTAAAGCG TTTTAAATCA ACGTTTAAAT TTTCTTTAAT GTTAGCTAAA TGAGATTTTT
     1321 TTTTTCAAAC TTACGAATTA TTACTTTTAA ACCTGTGATA CGGTTCAAAG TAAGTGGATA
     1381 TACACAAGAA GTCAAATAGC TCCCGTATTT TCATACAACA ACACCATCTT TCTTGAAAAA
     1441 ATAGACCATA TTTTTCGAGG TGATCTCTCG TTAGTATCCG TCAAGCTTTC ATCTTTATGT
     1501 TAGCACTTTT TTTCTTGGTT CAACTTCAAT TTTCCTCTAG ATTGTTCTAG TTGTTTTGAT
     1561 TTGTCTCAAG TCGATGTTAA AGTTTACATG TTTTCCTATC CGGAGTTGAT ATGGCGGTGA
     1621 ATGATAGTTG GTTCGATTTG ATGGTGTTCT TGTTCGTGAG ACAGATTTTT TTCGAGTAGT
     1681 TAAGCTTCGC TCCTTGTCTT TGATTTGCAT ATTTGGTAGT GATTTGATAG CGTTTTGTAG
     1741 AATTTTATTT AGACGTAAAT TGGTGGCATA TTTATTGTAA TTTTACATAT CAGATTATGA
     1801 GTTTGAATGG TAACACAATA CAGCTTTATA TAATTTTGTA CAACTTTCAC AGTTACCAAT
     1861 AACAAAAACT TTATTAAAAG TGTTATAAAA AGTTTACTCT CAGCTTTTTT GTACAACTCT
     1921 TTCTAACAGC TTTGCACTAT TCAAATAAAG CTAACAGCTA AAAAACTTGT ACAGCTTTTG
     1981 GTTACCAATC AGACACTATG TAAATTTTTG GTTTTTGCTT TTGTTACTTT TGAGAGGTCG
     2041 GCTCTTTTCT TGATAGGATA AAATTGTTGT TATTTTTGAG TTTGCTAGCA TCAATAATCA
     2101 CAATTAAGAT GTATCTTATT TTGAGAGGTC GGCTCTTTCT TTGATAGGAT AAAATTGTTG
     2161 TTATTTTTAA GTTTGCTGAG AGAATTCAAG ATGATAAAAA AATTAAAAAA ATCACAATCA
     2221 CAAGGTTTTA ATAATAAGGT TTCAAATATA AACTTTTTTT TTTTTGCACA AACTTTCAAA
     2281 TATAAACAAA AAAATAATTT TAATACAAAG TCTATATATG GAGAAAATAG GAAGCCAAAA
     2341 TTGATAATTC ACAAAATTAA AGTAAAATCT ATTAGCCGAC AAAAAAAAAG GTAAAATCTA
     2401 TTAAAATATA GATAAGGTTC TAGAAATTAA ATAAATCTAT TACCAATTCT CAAACCGACA
     2461 TAAGTACGAC CAAAAAAAAG TTTATAAATA AAAGTCACAA CGAGCCTTAA CGCGTAGAAT
     2521 CTTCCCGTAC TTTACTTTTC CGGAGGAATA GAAAATTGGG GGCTAGGGTT CGCAATTGTA
     2581 GTTTTCGAGC GAAGAAaatg tgagaccacg aagtggctct tcagtggacg aaagggcctc
     2641 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2701 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2761 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2821 aagagtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     2881 gcagtggcgg ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc
     2941 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     3001 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
     3061 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     3121 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     3181 acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     3241 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     3301 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     3361 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     3421 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     3481 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     3541 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     3601 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     3661 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     3721 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     3781 agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac
     3841 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3901 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3961 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//