LOCUS       pICSL12055              4553 bp ds-DNA     circular     07-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     repeat_region   complement(749..760)
                     /label="AAV2 ITR (fragment)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
     misc_feature    837..3144
                     /label="Suc2 Promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        3266..3370
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3361..3441)
                     /label="INT2_ECOLX (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             3529..3585
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             3592..4380
                     /label="S3AD_ECOLX"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 GGGTGCATAA TGATGGAACA AAGCACAAAT CTTTTAACGC AAACTAACTA CAACCTTCTT
      901 TTGGGGTCCC CATCCCCGAC CCTAATGTTT TGGAATTAAT AAAACTACAA TCACTTACCA
      961 AAAAATAAAA GTTCAAGGCC ACTATAATTT CTCATATGAA CCTACATTTA TAAATAAAAT
     1021 CTGGTTTCAT ATTAATTTCA CACACCAAGT TACTTTCTAT TATTAACTGT TATAATGGAC
     1081 CATGAAATCA TTTGCATATG AACTGCAATG ATACATAATC CACTTTGTTT TGTGGGAGAC
     1141 ATTTACCAGA TTTCGGTAAA TTGGTATTCC CCCTTTTATG TGATTGGTCA TTGATCATTG
     1201 TTAGTGGCCA GACATTTGAA CTCCCGTTTT TTTGTCTATA AGAATTCGGA AACATATAGT
     1261 ATCCTTTGAA AACGGAGAAA CAAATAACAA TGTGGACAAA CTAGATATAA TTTCAACACA
     1321 AGACTATGGG AATGATTTTA CCCACTAATT ATAATCCGAT CACAAGGTTT CAACGAACTA
     1381 GTTTTCCAGA TATCAACCAA ATTTACTTTG GAATTAAACT AACTTAAAAC TAATTGGTTG
     1441 TTCGTAAATG GTGCTTTTTA TTTTTGCGGA TGTTAGTAAA GGGTTTTATG TATTTTATAT
     1501 TATTAGTTAT CTGTTTTCAG TGTTATGTTG TCTCATCCAT AAAGTTTATA TGTTTTTTCT
     1561 TTGCTCTATA ACTTATATAT ATATATGAGT TTACAGTTAT ATTTATACAT TTCAGATACT
     1621 TGATCGGCAT TTTTTTTGGT AAAAAATATA TGCATGAAAA ACTCAAGTGT TTCTTTTTTA
     1681 AGGAATTTTT AAATGGTGAT TATATGAATA TAATCATATG TATATCCGTA TATATATGTA
     1741 GCCAGATAGT TAATTATTTG GGGGATATTT GAATTATTAA TGTTATAATA TTCTTTCTTT
     1801 TGACTCGTCT GGTTAAATTA AAGAACAAAA AAAACACATA CTTTTACTGT TTTAAAAGGT
     1861 TAAATTAACA TAATTTATTG ATTACAAGTG TCAAGTCCAT GACATTGCAT GTAGGTTCGA
     1921 GACTTCAGAG ATAACGGAAG AGATCGATAA TTGTGATCGT AACATCCAGA TATGTATGTT
     1981 TAATTTTCAT TTAGATGTGG ATCAGAGAAG ATAAGTCAAA CTGTCTTAAT AATTTAAGAC
     2041 AACCTCTTTT AATATTTTCC CAAAACATGT TTTATGTAAC TACTTTGCTT ATGTGATTGC
     2101 CTGAGGATAC TATTATTCTC TGTCTTTATT CTCTTCACAC CACATTTAAA TAGTTTAAGA
     2161 GCATAGAAAT TAATTATTTT CAAAAAGGTG ATTATATGCA TGCAAAATAG CACACCATTT
     2221 ATGTTTATAT TTTCAAATTA TTTAATACAT TTCAATATTT CATAAGTGTG ATTTTTTATT
     2281 TTTTTGTCAA TTTCATAAGT GTGATTTGTC ATTTGTATTA AACAATTGTA TCGCGCAGTA
     2341 CAAATAAACA GTGGGAGAGG TGAAAATGCA GTTATAAAAC TGTCCAATAA TTTACTAACA
     2401 CATTTAAATA TCTAAAAAGA GTGTTTCAAA AAAAATTCTT TTGAAATAAG AAAAGTGATA
     2461 GATATTTTTA CGCTTTCGTC TGAAAATAAA ACAATAATAG TTTATTAGAA AAATGTTATC
     2521 ACCGAAAATT ATTCTAGTGC CACTCGCTCG GATCGAAATT CGAAAGTTAT ATTCTTTCTC
     2581 TTTACCTAAT ATAAAAATCA CAAGAAAAAT CAATCCGAAT ATATCTATCA ACATAGTATA
     2641 TGCCCTTACA TATTGTTTCT GACTTTTCTC TATCCGAATT TCTCGCTTCA TGGTTTTTTT
     2701 TTAACATATT CTCATTTAAT TTTCATTACT ATTATATAAC TAAAAGATGG AAATAAAATA
     2761 AAGTGTCTTT GAGAATCGAA CGTCCATATC AGTAAGATAG TTTGTGTGAA GGTAAAATCT
     2821 AAAAGATTTA AGTTCCAAAA ACAGAAAATA ATATATTACG CTAAAAAAGA AGAAAATAAT
     2881 TAAATACAAA ACAGAAAAAA ATAATATACG ACAGACACGT GTCACGAAGA TACCCTACGC
     2941 TATAGACACA GCTCTGTTTT CTCTTTTCTA TGCCTCAAGG CTCTCTTAAC TTCACTGTCT
     3001 CCTCTTCGGA TAATCCTATC CTTCTCTTCC TATAAATACC TCTCCACTCT TCCTCTTCCT
     3061 CCACCACTAC AACCACCGCA ACAACCACCA AAAACCCTCT CAAAGAAATT TCTTTTTTTT
     3121 CTTACTTTCT TGGTTTGTCA aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg
     3181 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3241 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3301 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3361 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     3421 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
     3481 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     3541 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     3601 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     3661 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     3721 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     3781 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     3841 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     3901 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     3961 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     4021 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     4081 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     4141 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     4201 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     4261 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     4321 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
     4381 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4441 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4501 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
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