LOCUS       pICSL12063              3107 bp ds-DNA     circular     07-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     repeat_region   complement(749..760)
                     /label="AAV2 ITR (fragment)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
     promoter        841..1627
                     /label="AtAct2Prom"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_fwdcolor="#346ee0"
                     /vntifkey=29
                     /locus_tag="AtAct2Prom"
                     /label="AtAct2Prom"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    1628..1694
                     /label="SSU301 Leader"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /locus_tag="SSU301 Leader"
                     /label="SSU301 Leader"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     promoter        1820..1924
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1915..1995)
                     /label="INT2_ECOLX (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             2083..2139
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             2146..2934
                     /label="S3AD_ECOLX"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 tcgacaaaat ttagaacgaa cttaattatg atctcaaata cattgataca tatctcatct
      901 agatctaggt tatcattatg taagaaagtt ttgacgaata tggcacgaca aaatggctag
      961 actcgatgta attggtatct caactcaaca ttatacttat accaaacatt agttagacaa
     1021 aatttaaaca actatttttt atgtatgcaa gagtcagcat atgtataatt gattcagaat
     1081 cgttttgacg agttcggatg tagtagtagc cattatttaa tgtacatact aatcgtgaat
     1141 agtgaatatg atgaaacatt gtatcttatt gtataaatat ccataaacac atcatgaaag
     1201 acactttctt tcacggtctg aattaattat gatacaattc taatagaaaa cgaattaaat
     1261 tacgttgaat tgtatgaaat ctaattgaac aagccaacca cgacgacgac taacgttgcc
     1321 tggattgact cggtttaagt taaccactaa aaaaacggag ctgtcatgta acacgcggat
     1381 cgagcaggtc acagtcatga agccatcaaa gcaaaagaac taatccaagg gctgagatga
     1441 ttaattagtt taaaaattag ttaacacgag ggaaaaggct gtctgacagc caggtcacgt
     1501 tatctttacc tgtggtcgaa atgattcgtg tctgtcgatt ttaattattt ttttgaaagg
     1561 ccgaaaataa agttgtaaga gataaacccg cctatataaa ttcatatatt ttcctctccg
     1621 ctttgaaTAC TAAAGTCAGT GTGAAGTGTT TAAAGGAAAA AGCTTTGGAA GAAGCAAAAA
     1681 TCTTCTAACT AATGaatgtg agaccacgaa gtggctcttc agtggacgaa agggcctcgt
     1741 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     1801 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1861 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1921 gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     1981 agtggcggtt ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg
     2041 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     2101 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg
     2161 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     2221 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     2281 agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     2341 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     2401 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     2461 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     2521 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     2581 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     2641 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     2701 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     2761 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     2821 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     2881 ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg
     2941 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3001 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//