LOCUS       pICSL13005              3580 bp ds-DNA     circular     07-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     repeat_region   749..760
                     /label="AAV2 ITR (fragment)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="pLannotate"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
     Promoter        841..2167
                     /label="AtUbi10"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="Ubiquitn 10 (Arabidopsis thaliana)"
     Misc._binding   2161..2161
                     /label="Start of transcription"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        2293..2397
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2388..2468)
                     /label="INT2_ECOLX (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             2556..2612
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             2619..3407
                     /label="S3AD_ECOLX"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 GTCGAGCTGC AGGTCAACGG ATCAGGATAT TCTTGTTTAA GATGTTGAAC TCTATGGAGG
      901 TTTGTATGAA CTGATGATCT AGGACCGGAT AAGTTCCCTT CTTCATAGCG AACTTATTCA
      961 AAGAATGTTT TGTGTATCAT TCTTGTTACA TTGTTATTAA TGAAAAAATA TTATTGGTCA
     1021 TTGGACTGAA CACGAGTGTT AAATATGGAC CAGGCCCCAA ATAAGATCCA TTGATATATG
     1081 AATTAAATAA CAAGAATAAA TCGAGTCACC AAACCACTTG CCTTTTTTAA CGAGACTTGT
     1141 TCACCAACTT GATACAAAAG TCATTATCCT ATGCAAATCA ATAATCATAC AAAAATATCC
     1201 AATAACACTA AAAAATTAAA AGAAATGGAT AATTTCACAA TATGTTATAC GATAAAGAAG
     1261 TTACTTTTCC AAGAAATTCA CTGATTTTAT AAGCCCACTT GCATTAGATA AATGGCAAAA
     1321 AAAAACAAAA AGGAAAAGAA ATAAAGCACG AAGAATTCTA GAAAATACGA AATACGCTTC
     1381 AATGCAGTGG GACCCACGGT TCAATTATTG CCAATTTTCA GCTCCACCGT ATATTTAAAA
     1441 AATAAAACGA TAATGCTAAA AAAATATAAA TCGTAACGAT CGTTAAATCT CAACGGCTGG
     1501 ATCTTATGAC GACCGTTAGA AATTGTGGTT GTCGACGAGT CAGTAATAAA CGGCGTCAAA
     1561 GTGGTTGCAG CCGGCACACA CGAGTCGTGT TTATCAACTC AAAGCACAAA TACTTTTCCT
     1621 CAACCTAAAA ATAAGGCAAT TAGCCAAAAA CAACTTTGCG TGTAAACAAC GCTCAATACA
     1681 CGTGTCATTT TATTATTAGC TATTGCTTCA CCGCCTTAGC TTTCTCGTGA CCTAGTCGTC
     1741 CTCGTCTTTT CTTCTTCTTC TTCTATAAAA CAATACCCAA AGAGCTCTTC TTCTTCACAA
     1801 TTCAGATTTC AATTTCTCAA AATCTTAAAA ACTTTCTCTC AATTCTCTCT ACCGTGATCA
     1861 AGGTAAATTT CTGTGTTCCT TATTCTCTCA AAATCTTCGA TTTTGTTTTC GTTCGATCCC
     1921 AATTTCGTAT ATGTTCTTTG GTTTAGATTC TGTTAATCTT AGATCGAACA CGATTTTCTG
     1981 GGTTTGATCG TTAGATATCA TCTTAATTCT CGATTAGGGT TTCATAGATA TCATCCGATT
     2041 TGTTCAAATA ATTTGAGTTT TGTCGAATAA TTACTCTTCG ATTTGTGATT TCTATCTAGA
     2101 TCTGGTGTTA GTTTCTAGTT TGTGCGATCG AATTTGTCGA TTAATCTGAG TTTTTCTGAT
     2161 TAACAGGcca ttgagaccac gaagtggctc ttcagtggac gaaagggcct cgtgatacgc
     2221 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2401 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2461 gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca
     2521 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     2581 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg
     2641 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     2701 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata
     2761 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     2821 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     2881 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     2941 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     3001 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     3061 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     3121 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     3181 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     3241 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     3301 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     3361 aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc
     3421 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3481 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3541 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//