LOCUS       pICH41258CP             3353 bp    DNA     circular     19-JAN-2023
DEFINITION  pL0V-SP/NT2 [AATG-AGGT].
ACCESSION   urn.local...xs-fups4oa
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|512293858|                                                 
            VNTDBDATE|512293858|                                               
            LSOWNER|                                                           
            VNTNAME|pICH41258|                                                 
            VNTAUTHORNAME|Carola Engler|                                       
            VNTAUTHOREML|engler@icongenetics.de|                               
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
            (SXF                                                               
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            2 2232 1 2830 0 0 "")     #1=(CRSite (CStringList) "BsaI" "ggtctc"
            8 12 2 2232 0 2830 1 0 "")     #2=(CRSite (CStringList) "BstXI"
            "ccannnnnntgg" 9 0 0 0 "")         #3=(CRSite (CStringList)
            "DraIII" "cacnnngtg" 7 0 2 1 0 2223 0 0 "")     #4=(CRSite
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                              #7=(CFSignal (CObList) "G to A" 62 0 0 1192 1192
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                                #8=(CFSignal (CObList) "ORI" 33 0 1 1984 1984 0
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            #11=(CFSignal (CObList) "ALPHA" 4 0 0 2409 2732 0 (CStringList)    
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FEATURES             Location/Qualifiers
     promoter        110..214
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             215..1225
                     /name="aadA CDS"
                     /gene="aadA"
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /note="confers resistance against aminoglycosides
                     (PF01909)"
                     /transl_table=11
     promoter        complement(315..343)
                     /gene="intI1"
                     /label="int1 promoter"
     terminator      complement(1375..1413)
                     /name="Tndr"
                     /note="rho independent terminator"
                     /label="Tndr"
     rep_origin      1416..1984
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication; ori"
                     /label="ori rep origin"
     misc_RNA        1432..1984
                     /name="RNA-II"
     terminator      complement(1433..1466)
                     /name="Tndr"
                     /note="rho independent terminator"
                     /label="Tndr"
     misc_RNA        complement(1435..1542)
                     /name="RNA-I"
     regulatory      1795..2173
                     /regulatory_class="other"
                     /note="ColE1 ori"
     misc_feature    2104..2167
                     /name="L-strand Y effector site"
                     /note="L-strand Y effector site"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
     misc_feature    2236..3337
                     /modified_by="kourelis"
                     /label="MCS BpiI AATG-AGGT"
     protein_bind    2355..2376
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP.; CAP binding site"
                     /Transferred_From="CAP binding site protein bind"
                     /Transferred_Similarity="100.00%"
                     /label="CAP binding site protein bind"
     promoter        2391..2421
                     /note="lac promoter; promoter for the E. coli lac operon"
                     /Transferred_From="lac promoter"
                     /Transferred_Similarity="100.00%"
                     /label="lac promoter"
     protein_bind    2429..2445
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).; lac
                     operator"
                     /Transferred_From="lac operator protein bind"
                     /Transferred_Similarity="100.00%"
                     /label="lac operator protein bind"
     CDS             2482..3147
                     /note="Acropora millepora GFP-like fluorescent
                     chromoprotein amilCP; absorption max 588 nm"
                     /transl_table=1
                     /Transferred_From="AAU06854"
                     /Transferred_Translation_Similarity="100.00%"
                     /label="amilCP CDS"
     terminator      3193..3238
                     /note="rrnB T1 terminator; rrnBT1 terminator;
                     transcription terminator T1 from the E. coli rrnB  gene"
                     /Transferred_From="rrnB T1 terminator; rrnB T1 terminator;
                     rrnBT1 terminator; rrnBT1 terminator"
                     /Transferred_Similarity="100.00%"
                     /label="rrnB T1 terminator; rrnB T1 terminator; rrnBT1
                     terminator; rrnBT1 terminator"
     terminator      3280..3303
                     /note="T7Te terminator; phage T7 early transcription
                     terminator"
                     /Transferred_From="T7Te terminator"
                     /Transferred_Similarity="100.00%"
                     /label="T7Te terminator"
ORIGIN      
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatgttgtc ttcgcagctg gcaccaatac gcaaaccgcc tctccccgcg
     2281 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2341 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2401 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag
     2461 agaaagagga gaaatactag aatgagcgtg atcgctaagc agatgaccta caaggtgtac
     2521 atgagcggaa ctgtgaacgg tcactacttc gaggttgagg gtgatggtaa gggtaagcct
     2581 tatgagggtg agcagactgt gaagcttact gtgactaagg gtggtcctct tcctttcgct
     2641 tgggatattt tgtctcctca gtgccagtac ggtagcatcc ctttcactaa gtaccctgag
     2701 gatatccctg attacgtgaa gcagtctttc ccagagggtt acacctggga gaggatcatg
     2761 aatttcgagg atggtgctgt gtgcaccgtg agcaacgatt cttctattca gggaaactgc
     2821 ttcatctacc acgtgaagtt ctccggtctg aacttccctc ctaatggtcc tgtgatgcag
     2881 aagaaaactc agggttggga gcctaacacc gagaggcttt ttgctaggga tggtatgctg
     2941 ctgggtaaca acttcatggc tctgaagctt gagggtggtg gacattacct ttgcgagttc
     3001 aagaccactt acaaggctaa gaaacctgtg aagatgcctg gttaccacta tgtggataga
     3061 aagctggatg tgaccaacca caacaaggat tacaccagcg ttgagcagtg cgagatctct
     3121 attgctagaa agcctgtggt ggcttaggct tcgctgatag tgctagtgta gatcgctact
     3181 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     3241 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     3301 ttctgcgttt ataacagaca agctgtgacg aagacaaagg ttgagaccac gaa
//
