LOCUS       pICSL41308-LC               4093 bp    DNA        circular     29-JUN-2023
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     CDS             215..1225
                     /vntifkey="4"
                     /locus_tag="Sm/Sp\no\DraIII"
                     /label="Sm/Sp\no\DraIII"
                     /ApEinfo_label="Sm/Sp\no\DraIII"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1386..3248
                     /dnas_title="pBR322"
                     /vntifkey="21"
                     /locus_tag="pBR322"
                     /label="pBR322"
                     /ApEinfo_label="pBR322"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mutation        1192..1192
                     /vntifkey="62"
                     /locus_tag="G\to\A"
                     /label="G\to\A"
                     /ApEinfo_label="G\to\A"
                     /ApEinfo_fwdcolor="#00DF87"
                     /ApEinfo_revcolor="#00DF87"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1386..3246
                     /note="pBR322"
                     /locus_tag="ALPHA"
                     /label="ALPHA"
                     /ApEinfo_label="ALPHA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        3527..3650
                     /vntifkey="30"
                     /locus_tag="P\lac\prom"
                     /label="P\lac\prom"
                     /ApEinfo_label="P\lac\prom"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3657..3980
                     /vntifkey="4"
                     /locus_tag="ALPHA(1)"
                     /label="ALPHA(1)"
                     /ApEinfo_label="ALPHA"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        3619..3619
                     /vntifkey="30"
                     /note="lac promoter"
                     /locus_tag="P(LAC)"
                     /label="P(LAC)"
                     /ApEinfo_label="P(LAC)"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3480..3483
                     /vntifkey="21"
                     /locus_tag="AATG"
                     /label="AATG"
                     /ApEinfo_label="AATG"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4078..4081
                     /vntifkey="21"
                     /locus_tag="GCTT"
                     /label="GCTT"
                     /ApEinfo_label="GCTT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     source          1386..3248
                     /dnas_title="pAGM32305"
                     /locus_tag="ALPHA(2)"
                     /label="ALPHA(2)"
                     /ApEinfo_label="ALPHA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaA
     1381 GATCCagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1441 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1501 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1561 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1621 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1681 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     1741 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1801 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1861 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1921 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1981 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2041 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2101 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2161 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2221 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2281 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     2341 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     2401 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     2461 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     2521 catccgctta cagacaagct gtgaccgact ccgggagctg catgtgtcag aggttttcac
     2581 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
     2641 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     2701 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     2761 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
     2821 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
     2881 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
     2941 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
     3001 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac
     3061 ggaaacccaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
     3121 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc
     3181 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc
     3241 tgcccgagCC TTtttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct gtggtctcaa
     3481 atgttgtctt cgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3541 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3601 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     3661 ccatgattac gccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga
     3721 gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3781 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     3841 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     3901 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     3961 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4021 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacg aagacaagct
     4081 ttgagaccac gaa    
//

