LOCUS       pICH47822_mRFP1         5464 bp    DNA     circular     06-DEC-2022
DEFINITION  pL1V-R3_mRFP1.
ACCESSION   urn.local...y5-fups4oa
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     misc_feature    complement(23..169)
                     /note="LB; left border. Identical to sequence near
                     agrocinopine synthase on Agrobacterium tumefaciens strain
                     1D1460 plasmid pTi1D1460 (CP032929 nucleotides 161,573 -
                     161,719). "
                     /label="LB; left border"
     misc_feature    complement(76..100)
                     /note="T-DNA left border sequence (Thole et al., (2007)
                     Plant Physiol 145:1211-1219)"
     rep_origin      182..893
                     /note="<html><body>incP origin of
                     replication</body></html>"
                     /Transferred_From="oriV variant"
                     /Transferred_Similarity="100.00%"
                     /label="oriV"
     misc_feature    304..311
                     /note="DnaA box"
     promoter        910..1014
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1015..1875
                     /gene="bla"
                     /function="ampicillin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AHK23082.1"
                     /db_xref="GI:588295035"
     terminator      complement(2025..2063)
                     /name="Tndr"
                     /note="rho independent terminator"
                     /label="Tndr"
     rep_origin      2066..2634
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication; ori"
                     /label="ori rep origin"
     misc_RNA        2082..2634
                     /name="RNA-II"
     terminator      complement(2083..2116)
                     /name="Tndr"
                     /note="rho independent terminator"
                     /label="Tndr"
     misc_RNA        complement(2085..2192)
                     /name="RNA-I"
     misc_feature    2680..4161
                     /note="trans-acting replication protein that binds to  and
                     activates oriV"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RK2 trfa (no Esp3I)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RK2 trfa
                     (<a href=""urn:local:.:1v3-bpzs9qr"">RK2 trfa (no
                     Esp3I)</a>)"
                     /label="RK2 trfa"
     CDS             2970..4118
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /note="trfA"
     misc_feature    4191..4316
                     /note="RB; Right border. Identical to sequence near
                     D-nopaline dehydrogenase on Agrobacterium tumefaciens
                     strain 1D1460 plasmid pTi1D1460. Partial RB overdrive
                     sequence (CP032929 nucleotides 186,435 - 186,560)."
                     /label="RB partial overdrive sequence"
     misc_feature    complement(4274..4298)
                     /note="T-DNA right border sequence (Thole et al., (2007)
                     Plant Physiol 145:1211-1219)"
     misc_feature    4344..5431
                     /created_by="kourelis"
                     /label="MCS BsaI GGAG-CGCT"
     terminator      complement(4361..4384)
                     /note="T7Te terminator; phage T7 early transcription
                     terminator"
                     /label="T7Te terminator"
     terminator      complement(4426..4471)
                     /note="rrnB T1 terminator; rrnBT1 terminator;
                     transcription terminator T1 from the E. coli rrnB  gene"
                     /label="rrnB T1 terminator; rrnB T1 terminator; rrnBT1
                     terminator; rrnBT1 terminator"
     CDS             complement(4516..5193)
                     /name="mRFP1"
                     /note="engineered variant of tetrameric red fluorescent
                     protein from Discosoma deposited in GenBank Accession
                     Number AAF03369"
                     /product="monomeric red fluorescent protein"
                     /transl_table=11
     protein_bind    complement(5229..5245)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).; lac
                     operator"
                     /label="lac operator protein bind"
     promoter        complement(5253..5283)
                     /note="lac promoter; promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    complement(5298..5319)
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP.; CAP binding site"
                     /label="CAP binding site protein bind"
ORIGIN      
        1 gtggggccca ctgcatccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg
       61 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
      121 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tcagatcagg
      181 atctcctttg cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat
      241 ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg
      301 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
      361 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
      421 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
      481 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
      541 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
      601 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
      661 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
      721 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
      781 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
      841 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
      901 caattcgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      961 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1021 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1081 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1141 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1201 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1261 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1321 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1381 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1441 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1501 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1561 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1621 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1681 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg ttctcgcggt
     1741 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1801 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1861 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2161 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2641 agcaacgcgg cctttttacg gttcctggca gatcctagat gtggcgcaac gatgccggcg
     2701 acaagcagga gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc
     2761 acggcaagta tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca
     2821 agtacgagaa ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt
     2881 atctggacac caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt
     2941 gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca
     3001 ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca
     3061 ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta
     3121 cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg
     3181 ccgccgtgga gcgttcgcgt cgtcttgaac aggaggcggc aggtttggcg aagtcgatga
     3241 ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg
     3301 caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca
     3361 aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc
     3421 caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg
     3481 cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg
     3541 tcgagctgcg ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc
     3601 gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct
     3661 ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga
     3721 cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc
     3781 gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg
     3841 aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca
     3901 agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc
     3961 cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt
     4021 ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg
     4081 cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg
     4141 ctgggggttc agcagccagc gcctgatctg gggaaccctg tggttggcat gcacatacaa
     4201 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
     4261 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaaccact
     4321 tcgtgcagaa gacaagtaag gagtgagacc tataaacgca gaaaggccca cccgaaggtg
     4381 agccagtgtg actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     4441 agtctttcga ctgagccttt cgttttattt gatgcctggc tctagtagcg atctacacta
     4501 gcactatcag cgttattaag caccggtgga gtgacgacct tcagcacgtt cgtactgttc
     4561 aacgatggtg tagtcctcgt tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc
     4621 acccggcagc tgaaccggtt ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg
     4681 accaccgtct ttcagtttca gacgcatttt gatttcacct ttcagagcac cgtcctccgg
     4741 gtacatacgt tcggtggaag cttcccaacc catggttttt ttctgcataa ccggaccgtc
     4801 ggacgggaag ttggtaccac gcagtttaac tttgtagatg aactcaccgt cttgcaggga
     4861 ggagtcctgg gtaacggtaa caacaccacc gtcctcgaag ttcataacac gttcccattt
     4921 gaaaccttcc gggaaggaca gtttcaggta gtccgggatg tcagccgggt gtttaacgta
     4981 agctttggaa ccgtactgga actgcgggga caggatgtcc caagcgaacg gcagcggacc
     5041 acctttggta actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc
     5101 accttcgatt tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat
     5161 gaactctttg ataacgtcct cggaggaagc catctagtat ttctcctctt tctctagtat
     5221 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5281 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5341 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5401 agaggcggtt tgcgtattga tatcggtctc acgctgagct cgaattctag tttgtcttca
     5461 caga
//
