LOCUS       Exported                3594 bp ds-DNA     circular SYN 11-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     570..665
                     /locus_tag="AttL1"
                     /label=AttL1
     misc_recomb     645..665
                     /locus_tag="attB1"
                     /label=attB1
     misc_feature    652..658
                     /locus_tag="Gateway recomb1"
                     /label=Gateway recomb1
     misc_feature    670..696
                     /locus_tag="5' homology arm"
                     /label=5' homology arm
     misc_feature    682
                     /locus_tag="PCR insertion between TA"
                     /label=PCR insertion between TA
     primer_bind     join(683..684,691..696)
                     /locus_tag="Forward primer"
                     /label=Forward primer
     misc_feature    703..732
                     /locus_tag="SGSGGSGGSG linker"
                     /label=SGSGGSGGSG linker
     misc_feature    733..1440
                     /locus_tag="mCherry"
                     /label=mCherry
     primer_bind     complement(802..821)
                     /locus_tag="oSC187 reverse"
                     /label=oSC187 reverse
     misc_feature    1444..1466
                     /locus_tag="3' homology arm"
                     /label=3' homology arm
     primer_bind     complement(join(1444..1449,1458..1459))
                     /locus_tag="Reverse primer"
                     /label=Reverse primer
     misc_feature    1460
                     /locus_tag="PCR insertion between TA(1)"
                     /label=PCR insertion between TA
                     /label=PCR insertion between TA(1)
     misc_recomb     complement(1476..1496)
                     /locus_tag="attB2"
                     /label=attB2
     misc_feature    complement(1483..1489)
                     /locus_tag="Gateway Recomb2"
                     /label=Gateway Recomb2
     primer_bind     complement(1589..1610)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(1609..1629)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     promoter        1707..1840
                     /locus_tag="SpnR Promoter"
                     /label=SpnR Promoter
     gene            1841..2851
                     /locus_tag="SpnR"
                     /label=SpnR
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttggactagt catatgggta ccagtggttc cggaggatct
      721 ggaggatctg gtatggtctc aaagggtgaa gaagataaca tggcaattat taaagagttt
      781 atgcgtttca aggtgcatat ggagggatct gtcaatgggc atgagtttga aattgaaggt
      841 gaaggagaag gccgaccata tgagggaaca caaaccgcaa aactaaaggt aactaaaggc
      901 ggaccattac cattcgcctg ggacatcctc tctccacagt tcatgtatgg aagtaaagct
      961 tatgttaaac atccggcaga tataccagat tatttgaaac tttcattccc ggagggtttt
     1021 aagtgggaac gcgtaatgaa ttttgaagac ggaggagttg ttacagtgac gcaagactca
     1081 agcctccaag atggagaatt tatttataaa gtcaaacttc gaggaacgaa tttcccctcg
     1141 gatggacctg ttatgcagaa gaagactatg ggatgggaag cttcaagtga aagaatgtac
     1201 cctgaagacg gtgctcttaa gggagagatt aaacaacgtc ttaaattgaa agatggagga
     1261 cattacgatg ctgaggtgaa gacaacttac aaagccaaaa aaccagttca gctgccagga
     1321 gcgtacaatg ttaatattaa actggatatc acctcccaca acgaggatta cactatcgtt
     1381 gagcaatatg aaagagctga agggcggcac tcgacaggtg gcatggatga attgtataag
     1441 tagcatatgg cggccgcgga agggcgaatt cgacccagct ttcttgtaca aagttggcat
     1501 tataaaaaat aattgctcat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1561 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     1621 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     1681 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     1741 gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     1801 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     1861 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1921 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     1981 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2041 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     2101 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     2161 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     2221 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     2281 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     2341 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     2401 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     2461 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     2521 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     2581 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     2641 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     2701 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     2761 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     2821 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     2881 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3061 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3301 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     3361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
