LOCUS       Exported                4191 bp ds-DNA     circular SYN 09-AUG-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     570..665
                     /locus_tag="AttL1"
                     /label=AttL1
     misc_recomb     645..665
                     /locus_tag="attB1"
                     /label=attB1
     misc_feature    652..658
                     /locus_tag="Gateway recomb1"
                     /label=Gateway recomb1
     misc_feature    670..696
                     /locus_tag="5' homology arm"
                     /label=5' homology arm
     misc_feature    682
                     /locus_tag="PCR insertion between TA"
                     /label=PCR insertion between TA
     primer_bind     join(683..684,691..696)
                     /locus_tag="Forward primer"
                     /label=Forward primer
     CDS             703..1284
                     /locus_tag="rac-2 cDNA"
                     /label=rac-2 cDNA
     misc_feature    1291..1320
                     /locus_tag="SGSGGSGGSG linker"
                     /label=SGSGGSGGSG linker
     gene            1321..2037
                     /locus_tag="mGFP"
                     /label=mGFP
     primer_bind     complement(1384..1403)
                     /locus_tag="oSC186 reverse"
                     /label=oSC186 reverse
     misc_feature    2041..2063
                     /locus_tag="3' homology arm"
                     /label=3' homology arm
     primer_bind     complement(join(2041..2046,2055..2056))
                     /locus_tag="Reverse primer"
                     /label=Reverse primer
     misc_feature    2057
                     /locus_tag="PCR insertion between TA(1)"
                     /label=PCR insertion between TA
                     /label=PCR insertion between TA(1)
     misc_recomb     complement(2073..2093)
                     /locus_tag="attB2"
                     /label=attB2
     misc_feature    complement(2080..2086)
                     /locus_tag="Gateway Recomb2"
                     /label=Gateway Recomb2
     primer_bind     complement(2186..2207)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(2206..2226)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     promoter        2304..2437
                     /locus_tag="SpnR Promoter"
                     /label=SpnR Promoter
     gene            2438..3448
                     /locus_tag="SpnR"
                     /label=SpnR
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttggactagt catatgggta ccatgcaagc aatcaaatgt
      721 gtcgtcgttg gtgacggagc cgtcggtaag acgtgtctcc tgctatcgta cactacaaac
      781 gcttttcccg gagaatatat tctaacggta ttcgacacct actcaacaaa tgtgatggtc
      841 gacggaaggc caataaatct cagcctatgg gacacagctg gacaggacga ttacgatcaa
      901 ttccgccacc tgtcatttcc acaaacagac gtattcctcg tatgctttgc actgaataat
      961 ccagcaagtt ttgagaatgt tcgtgcaaaa tggtatccag aagtatcaca tcattgccca
     1021 aatacaccga ttattttggt tggaactaaa gctgatttac gcgaggatcg agatactatt
     1081 gaacggctcc gtgaacgtcg gctccaacca gtgagccaca cccagggtta cgtgatggca
     1141 aaggaaatca aggcggtcaa gtacctggaa tgctcggcgc ttacccaaat tggattgaaa
     1201 caagttttcg atgaggcaat tcgtactggg ctcaccccgc cacaaacacc acaaacgaga
     1261 gccaaaaaga gcaattgcac ggtgggtacc agtggttccg gaggatctgg aggatctggt
     1321 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1381 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1441 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1501 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1561 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1621 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1681 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1741 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1801 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1861 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1921 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1981 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtag
     2041 catatggcgg ccgcggaagg gcgaattcga cccagctttc ttgtacaaag ttggcattat
     2101 aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2161 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2221 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2281 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctgcc
     2341 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     2401 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     2461 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     2521 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     2581 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     2641 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     2701 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     2761 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     2821 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     2881 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     2941 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     3001 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     3061 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     3121 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     3181 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     3241 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     3301 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     3361 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     3421 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     3481 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3541 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3601 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3661 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3721 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3781 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3841 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3901 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     3961 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4021 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4081 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4141 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
