LOCUS       KB35_2                  5572 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  KB35_2.
ACCESSION   KB35_2
VERSION     KB35_2
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             4207..5085
                     /note="pLannotate"
                     /label="araC"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2473..3264
                     /note="pLannotate"
                     /label="SmR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             271..1221
                     /note="pLannotate"
                     /label="Rep101"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..223
                     /note="pLannotate"
                     /label="pSC101 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(3896..4180)
                     /note="pLannotate"
                     /label="araBAD promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3548..3814)
                     /note="pLannotate"
                     /label="ASIA_BPT4"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="98.9"
                     /fragment="False"
                     /other="CDS"
     RBS             complement(3847..3869)
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     CDS             complement(1752..2324)
                     /note="pLannotate"
                     /label="INT2_ECOLX (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="56.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(3512..3535)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="42.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             2410..2466
                     /note="pLannotate"
                     /label="GmR (fragment)"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
     CDS             3428..3520
                     /note="pLannotate"
                     /label="QACE_ECOLX (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="28.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3815..3834)
                     /note="pLannotate"
                     /label="capTEV™ (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
       61 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
      121 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
      181 acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa ttggtatagt
      241 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
      301 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
      361 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
      421 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
      481 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
      541 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
      601 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
      661 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
      721 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
      781 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
      841 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
      901 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
      961 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     1021 acatcagatt cctacctaca taacggacta agaaaaacac tacacgatgc tttaactgca
     1081 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagtatgat
     1141 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
     1201 gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg aagcatcaag
     1261 actaacaaac aaaagtagaa caactgttca ccgttacata tcaaagggaa aactgtccat
     1321 atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac gagtttttgg
     1381 tgcattcaaa gctgttcacc atgaacagat cgacaatgta acagatgaac agcatgtaac
     1441 acctaataga acaggtgaaa ccagtaaaac aaagcaacta gaacatgaaa ttgaacacct
     1501 gagacaactt gttacagctc aacagtcaca catagacagc ctgaaacagg cgatgctgct
     1561 tatcgaatca aagctgccga caacacggga gccagtgacg cctcccgtgg ggaaaaaatc
     1621 atggcaattc tggaagaaat agcgctttca gccggcaaac cggctgaagc cggatctgcg
     1681 attctgataa caaactagca acaccagaac agcccgtttg cgggcagcaa aacccgtggg
     1741 aattaattcc cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga
     1801 tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg
     1861 acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac
     1921 aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc
     1981 accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg
     2041 cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc
     2101 gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag
     2161 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     2221 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     2281 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     2341 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     2401 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     2461 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     2521 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2581 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     2641 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     2701 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2761 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     2821 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     2881 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     2941 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     3001 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     3061 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     3121 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     3181 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     3241 atcaccaagg tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg
     3301 gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
     3361 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
     3421 atatatcatg aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac
     3481 atccgcatta aaatctagcg agggctttac taagcttgca tgcctgcagg tcgacctact
     3541 gcagttattt gttggtatac atttccagat agcgatacac accttcaaag ccttcgttaa
     3601 attcatcgat cagggttttt ttgtcctctt gggtcagttc gctcacaatt ttgcgaaagc
     3661 tattctgatt cagtttacga ccttcatggg tcacgccaat ttcattcaga aaggcaataa
     3721 agtttgcacg gttttccacg atatcttcac ggctaaattt gatcagaatg cttgcaacgg
     3781 tgataatttc acgcacggta tcaatgtttt tgttatgatg atgatgatga tgcatgctag
     3841 cgtatatctc cttcttaaag ttaaacaaaa ttatttctag cccaaaaaaa cgggtatgga
     3901 gaaacagtag agagttgcga taaaaagcgt caggtaggat ccgctaatct tatggataaa
     3961 aatgctatgg catagcaaag tgtgacgccg tgcaaataat caatgtggac ttttttgccg
     4021 tgattataga cacttttgtt acgcgttttt gtcatggctt tggtcccgct ttgttacaga
     4081 atgcttttaa taagcggggt taccggtttg gttagcgaga agagccagta aaagacgcag
     4141 tgacggcaat gtctgatgca atatggacaa ttggtttctt ctctgaatgg cgggagtatg
     4201 aaaagtatgg ctgaagcgca aaatgatccc ctgctgccgg gatactcgtt taatgcccat
     4261 ctggtggcgg gtttaacgcc gattgaggcc aacggttatc tcgatttttt tatcgaccga
     4321 ccgctgggaa tgaaaggtta tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa
     4381 aatcagggac gagaatttgt ttgccgaccg ggtgatattt tgctgttccc gccaggagag
     4441 attcatcact acggtcgtca tccggaggct cgcgaatggt atcaccagtg ggtttacttt
     4501 cgtccgcgcg cctactggca tgaatggctt aactggccgt caatatttgc caatacgggg
     4561 ttctttcgcc cggatgaagc gcaccagccg catttcagcg acctgtttgg gcaaatcatt
     4621 aacgccgggc aaggggaagg gcgctattcg gagctgctgg cgataaatct gcttgagcaa
     4681 ttgttactgc ggcgcatgga agcgattaac gagtcgctcc atccaccgat ggataatcgg
     4741 gtacgcgagg cttgtcagta catcagcgat cacctggcag acagcaattt tgatatcgcc
     4801 agcgtcgcac agcatgtttg cttgtcgccg tcgcgtctgt cacatctttt ccgccagcag
     4861 ttagggatta gcgtcttaag ctggcgcgag gaccaacgta tcagccaggc gaagctgctt
     4921 ttgagcacca cccggatgcc tatcgccacc gtcggtcgca atgttggttt tgacgatcaa
     4981 ctctatttct cgcgggtatt taaaaaatgc accggggcca gcccgagcga gttccgtgcc
     5041 ggttgtgaag aaaaagtgaa tgatgtagcc gtcaagttgt cataagaatt cccgacagta
     5101 agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc agtctgaatg
     5161 acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag gaactgctga
     5221 acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc tgagaagccc
     5281 gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa aaggcgcctg
     5341 tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg aatgtcacga
     5401 aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca gcgatttgcc
     5461 cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt gtagaggagc
     5521 aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta gt
//
