LOCUS       Exported                5735 bp ds-DNA     circular SYN 14-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   AenigO16
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     source          1762..5735
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     misc_binding    177..199
                     /label=LacO
     primer_bind     205..225
                     /label=M13-rev
     misc_feature    350..1761
                     /label=16S_AenigO16
     primer_bind     complement(1768..1795)
                     /label=T7
     primer_bind     complement(1794..1811)
                     /label=M13-fwd
     CDS             1882..1950
                     /codon_start=1
                     /label=LacZ alpha
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     misc_feature    1935..1975
                     /label=AS1334
     CDS             complement(1956..2355)
                     /codon_start=1
                     /gene="mob"
                     /product="transfer origin"
                     /label=transfer origin
                     /translation="FLHLPVSSR*IFHKTAGKQRFSAA*PCFGVIIAIFSVYPSFFARY
                     TGFCQRVRVDFPWCIQRRQPGRIGEVGPPASGCSFFTVPYSHLAVLNGNPALRGWPATA
                     GVTDEGKRMADETKPTRKGSPPIKVYCLP"
     rep_origin      2368..2674
                     /label=F1 ori
     CDS             4133..4792
                     /codon_start=1
                     /label=AmpR
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      4890..5572
                     /label=ColE1 origin
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagaa ttgtaatacg
      361 actcactata gggcgaatta gatcactgct atcaggttcc gactaagcca tgcgaggcgt
      421 gagctatctg ggctcgccga actgctgagt aacacgtagt taacctgacc tcaagtaggg
      481 gataatctcg ggaaactgag gctaataccc tatagttgtg aaatgctgga atgcgttcac
      541 agcgaaatct acggagcttg aggatgggac tgcgacgtat caggttgatg cgagtgtaaa
      601 gtactcacat gcctataacg cgtaggggtt atgagagtaa aagcctccag atgccttctg
      661 agacatgaag gcaggtccta cgggacgcag caggcgcgaa acctttccaa ttcgttagcg
      721 cgtgagaagg ggagcttgag tggttatgca atgcataacc ttttctcttg tctaaaaaac
      781 aaggggaata agtggtgggc aagacgggtg ccagccgccg cggtaatacc cgcgccacga
      841 gtggtgatct cgtttattgg gtctaaagag agcgtagccg gtctgacaag tctctggtga
      901 aatctggcag ctaactgtca ggcgtgctgg agatactatc aggcttggga gcgggaaagg
      961 atggaggtat tccacgggta ggagttaaat cctttaatcc gtggaggacc accagtggcg
     1021 aaggcgtcca tccggaacgc gtccgacggt gaggctcgaa agcgtgggga gcaaatggga
     1081 ttagataccc cagtagtcca cgctgtaaac gatgtccctt tggtgttgca ttctctacgg
     1141 gagactgcag tgccggagcg taggtgttaa agggaccacc tggggagtac ggtcgcaaga
     1201 ctgaaactta agggaattgg cgggggagca cacaaggggt ggaggctgcg gttcaattgg
     1261 attcaacgcc ggacacctta ctcgcgcaga cagcagaatg aaggtcagcc tgacgggttt
     1321 accagacaag ctgagaggta ttgcatggcc atcgtcagct cgtaccatga ggcgtgccgt
     1381 taagtcggtt aacgagcgag acccacacca ccagttgcaa tcacaatccc aggagaaatc
     1441 caaaagattg tgagcactct ggtaggaccg ctgctgctaa agcagaggaa ggagtgggcg
     1501 acggtaggtc cgtatggtcc gattgcgcga ggctacacgc ggcctacaat ggttaggaca
     1561 atgtgatgcc actccgaaag gaggagctaa tcacctaaac ctagccctag ttcagattga
     1621 gggctgcaac tcgccctcat gaagctggaa tccctagtaa gcgaacgtca taatcgttcg
     1681 ctgctagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gtgcatgctc
     1741 gagcggccgc cagtgtgatg gaattcgccc tatagtgagt cgtattacaa ttcactggcc
     1801 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1861 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1921 caacagttgc gcagcctgaa tggcgaatgg acgcgctgga aggcagtaca ccttgatagg
     1981 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     2041 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     2101 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     2161 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     2221 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     2281 ttatgcagcg gaaaagcgct gcttccctgc tgttttgtgg aatatctacc gactggaaac
     2341 aggcaaatgc aggaaccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2401 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2461 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2521 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2581 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2641 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2701 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2761 caaaaattta acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga
     2821 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     2881 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     2941 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     3001 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     3061 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc
     3121 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3181 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3241 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3301 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3361 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3421 aggatctcct gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     3481 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     3541 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3601 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     3661 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3721 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     3781 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     3841 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     3901 ttgacgagtt cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3961 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4021 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4081 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4141 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4201 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4261 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4321 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4381 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4441 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4501 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4561 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4621 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4681 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4741 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4801 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4861 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4921 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4981 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5041 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5101 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5161 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5221 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5281 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5341 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5401 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5461 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5521 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5581 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5641 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5701 cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
