LOCUS       Exported                5900 bp ds-DNA     circular SYN 14-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   AltiSM1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     source          1927..5900
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     misc_binding    177..199
                     /label=LacO
     primer_bind     205..225
                     /label=M13-rev
     misc_feature    350..1926
                     /label=16S_AltiSM1
     primer_bind     complement(1933..1960)
                     /label=T7
     primer_bind     complement(1959..1976)
                     /label=M13-fwd
     CDS             2047..2115
                     /codon_start=1
                     /label=LacZ alpha
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     misc_feature    2100..2140
                     /label=AS1334
     CDS             complement(2121..2520)
                     /codon_start=1
                     /gene="mob"
                     /product="transfer origin"
                     /label=transfer origin
                     /translation="FLHLPVSSR*IFHKTAGKQRFSAA*PCFGVIIAIFSVYPSFFARY
                     TGFCQRVRVDFPWCIQRRQPGRIGEVGPPASGCSFFTVPYSHLAVLNGNPALRGWPATA
                     GVTDEGKRMADETKPTRKGSPPIKVYCLP"
     rep_origin      2533..2839
                     /label=F1 ori
     CDS             4298..4957
                     /codon_start=1
                     /label=AmpR
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      5055..5737
                     /label=ColE1 origin
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagaa ttgtaatacg
      361 actcactata gggcgaatta ctccagttga tcctgctggc ggctactgct cttggggttc
      421 gattaagcca tgtgagtcca ggaatctttg attcctggcg aacgactcag taacacgtgg
      481 ataacctgcc cttaggaaga gtataacctc gggaaactga ggacaatcct ctataggtga
      541 gaagttctgg aatgactctc actcaaagct tcggcgccta aggatgggtc tgcggtggat
      601 taggctgatg gcggggtaac ggcccaccat acctataatc cataccggct atgggagtag
      661 gagccgggag atgagtactg agacacggac tcaggcccta cggggcgcat caggcgcgaa
      721 acctttgcaa tgcacgcaag tgtgacaagg gaaccctgag tgtcttctct ttgaggggac
      781 ttttatgatg tgcaaaaagc atcatgaata agggctgggt aagacccgtg ccagccgccg
      841 cggtaacacg ggcagcccaa gtggtagcca tgtttattgg gcttaaagcg ttcgtagccg
      901 gtctggtaag tcctccgtga aatcctgtcg cttaacgata ggaattgcag aggatactac
      961 ctgacttgga gccgggatgc gtcagaggta ttccttgggt aggggcgaaa tccgttgatc
     1021 cctggaggac caccaacggc gaaggcatct gacttgaacg ggctcgacgg tgaggaacga
     1081 aagctagggg agcaaaccgg atcagatacc cgggtagtcc tagccgtaaa cgatgcggac
     1141 tgtatgttgc taaagcttta tgttttagca gtgtcgaagg gaaaccgtta agtccgccgc
     1201 ctgggaagta cggtcgcaag gctgaaactt aaaggaattg gcgggggatc accacaaggg
     1261 gtggagtctg cggttcaatt ggatacaaca ccgggaaact taccagggac gacggcagga
     1321 tgaaggtcag gctgaaggtc ttaccgaacg agccgagagg tagtgcatgg ccatcgacag
     1381 ctcgtaccgt gaggcgtgcg gttaagtccg ctaacgagca agacccgtgc ctttatttgc
     1441 cacccttttc ttttgaagag gagcactata aagggaccgc tggcgataag tcagaggaag
     1501 gtgcgggcga cggtaggtct gtatgctccg aatttcctgg gctacacgtg gactacaatg
     1561 gcacaaacag cgggatgcaa ctccgagagg agaagccaat ctcttaaatt gtgtcttagt
     1621 tcggattgag ccttgaaact cgggctcatg aagttgaaat ccctagtaat cgctctttat
     1681 tagagagcgg tgaatatgtc cccgatcctt gcacacaccg cctgtcaaac caatcgagtg
     1741 gacttaggtc gaggctctct catctgggaa ggtcaagatc taggcttgca agaggggtta
     1801 agtcataaca aggtagccgt aggggaatct gcggctggat cacctccact agcataaccc
     1861 cttggggcct ctaaacgggt cttgaggggt tttttgtgca tgctcgagcg gccgccagtg
     1921 tgatggaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
     1981 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2041 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2101 ctgaatggcg aatggacgcg ctggaaggca gtacaccttg ataggtgggc tgcccttcct
     2161 ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg
     2221 ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa
     2281 gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg
     2341 gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa
     2401 ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa
     2461 gcgctgcttc cctgctgttt tgtggaatat ctaccgactg gaaacaggca aatgcaggaa
     2521 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2581 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2641 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2701 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     2761 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2821 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2881 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2941 aattttaaca aaattcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca
     3001 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     3061 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     3121 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     3181 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     3241 gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3301 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3361 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3421 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     3481 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3541 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3601 cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3661 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3721 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3781 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     3841 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3901 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     3961 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4021 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4081 gaattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4141 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4201 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4261 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4321 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4381 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4441 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4501 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4561 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4621 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4681 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4741 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4801 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4861 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4921 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4981 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5041 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5101 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5161 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5221 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5281 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5341 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5401 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5461 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5521 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5581 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5641 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5701 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5761 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5821 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5881 agtgagcgag gaagcggaag
//
