LOCUS       Exported                5933 bp ds-DNA     circular SYN 14-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   Culex-027
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     source          1960..5933
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     misc_binding    177..199
                     /label=LacO
     primer_bind     205..225
                     /label=M13-rev
     misc_feature    350..1959
                     /label=16S_Culex_027
     primer_bind     complement(1966..1993)
                     /label=T7
     primer_bind     complement(1992..2009)
                     /label=M13-fwd
     CDS             2080..2148
                     /codon_start=1
                     /label=LacZ alpha
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     misc_feature    2133..2173
                     /label=AS1334
     CDS             complement(2154..2553)
                     /codon_start=1
                     /gene="mob"
                     /product="transfer origin"
                     /label=transfer origin
                     /translation="FLHLPVSSR*IFHKTAGKQRFSAA*PCFGVIIAIFSVYPSFFARY
                     TGFCQRVRVDFPWCIQRRQPGRIGEVGPPASGCSFFTVPYSHLAVLNGNPALRGWPATA
                     GVTDEGKRMADETKPTRKGSPPIKVYCLP"
     rep_origin      2566..2872
                     /label=F1 ori
     CDS             4331..4990
                     /codon_start=1
                     /label=AmpR
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      5088..5770
                     /label=ColE1 origin
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagaa ttgtaatacg
      361 actcactata gggcgaatta ccccggttga tcctgccgga cccgactgct atgggggtgg
      421 gactaagcca tgcgagtcag caccccaggc cacgttgggg tgcggcgtac ggctcagtaa
      481 aacgtggcta acctacccta gggatgggga taaccccggg aaactgggga taatccccaa
      541 taggagaaga gtgctggaat gcctcttctc cgaaaggatg ccaagaacat gcctcttggc
      601 atcgccctag gatggggcca cgtcccatca ggtagttggt ggggtaacgg cccaccaagc
      661 ctataacggg tacgggccgt gaaagcggga gcccggagat gggcactgag acaagggccc
      721 aggccctacg gggcgcagca ggcgcgaaac ctacacaatg tgcgaaagca cgatgtggtc
      781 acccccagtg ccacccgctg agggtggctt ttctccggtg taaaaagccg gaggcaataa
      841 gcggggggca agtctggtgt cagccgccgc ggtaatacca gctccgcgag tggtcggggc
      901 gtttattggg cctaaagcgt ccgtagccgg cccggtaagt tttcagctaa agcccagggc
      961 tcaaccctgg gaaggctgaa aatactgccg ggctaggggg cgggagaggc tgagggtact
     1021 cctggggtag gggcgaaatc ctataatccc aggaggacca ccagtggcga aggcgctcag
     1081 ctggaacgcg cccgacggtg agggacgaaa gccgggggag cgaaggggat tagatacccc
     1141 cgtagtcccg gctgtaaacg atgcgggcta ggtgttaggt ggaccttgtg tccacctagt
     1201 gccgcaggga agccgttaag cccgccgcct ggggagtacg gccgcaaggc tgaaacttaa
     1261 aggaattggc gggggagcac cacaaggggt gaagcctgcg gtttaattgg agtcaacccc
     1321 gagaacctta ccgggggcga cagcaggatg aaggccaggc tgacgacctt gccagacgag
     1381 ctgagaggag gtgcatggcc gtcgccagtt cgtgccgtga ggtgtccggt taagtccggc
     1441 aacgaacaag acccccacgt ttagttgcta cttagcccta gtaagggcta aggcacacta
     1501 aacggactgc cgccgctaag gcggaggaag gagggggcca cggcaggtcc gtatgccccg
     1561 aaacccccgg gccacacgcg ggctgcaatg gtggggacaa tgggttccga ccccgaaagg
     1621 ggaaggtaat cccataaacc ccacctcagt tgggatcgag ggctgcaact cgccctcgtg
     1681 aacatggaat ccctagtaac cgcgcgtcac caacgcgcgg tgaatacgtc cctgctcctt
     1741 gcacacaccg cccgtcgctc catccgagtc gggcttagat gagctctggc tcgcaaaggg
     1801 tcagggcgaa tctaagctcg atgaggaggg agaagtcgta acaaggtggc cgtaggggaa
     1861 cctgcggccg gatcacctcc tctagcataa ccccttgggg cctctaaacg ggtcttgagg
     1921 ggttttttgt gcatgctcga gcggccgcca gtgtgatgga attcgcccta tagtgagtcg
     1981 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2041 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2101 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgctggaag
     2161 gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat cagccatccg
     2221 cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt
     2281 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
     2341 tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac
     2401 cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat
     2461 aatgaccccg aagcagggtt atgcagcgga aaagcgctgc ttccctgctg ttttgtggaa
     2521 tatctaccga ctggaaacag gcaaatgcag gaaccctgta gcggcgcatt aagcgcggcg
     2581 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     2641 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2701 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2761 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2821 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2881 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2941 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg
     3001 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     3061 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     3121 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     3181 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     3241 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
     3301 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3361 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3421 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3481 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     3541 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3601 tgggcgaagt gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat
     3661 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3721 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3781 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3841 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     3901 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     3961 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4021 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4081 tcgccttcta tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgagtat
     4141 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4201 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4261 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4321 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4381 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4441 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4501 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4561 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4621 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4681 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4741 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4801 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4861 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4921 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4981 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5041 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5101 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5161 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5221 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5281 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5341 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5401 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5461 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5521 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5581 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5641 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5701 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5761 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5821 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5881 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
