LOCUS       Exported                5914 bp ds-DNA     circular SYN 14-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   DiapAR10
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     source          1941..5914
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     misc_binding    177..199
                     /label=LacO
     primer_bind     205..225
                     /label=M13-rev
     misc_feature    350..1940
                     /label=16S_DiapAR10
     primer_bind     complement(1947..1974)
                     /label=T7
     primer_bind     complement(1973..1990)
                     /label=M13-fwd
     CDS             2061..2129
                     /codon_start=1
                     /label=LacZ alpha
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     misc_feature    2114..2154
                     /label=AS1334
     CDS             complement(2135..2534)
                     /codon_start=1
                     /gene="mob"
                     /product="transfer origin"
                     /label=transfer origin
                     /translation="FLHLPVSSR*IFHKTAGKQRFSAA*PCFGVIIAIFSVYPSFFARY
                     TGFCQRVRVDFPWCIQRRQPGRIGEVGPPASGCSFFTVPYSHLAVLNGNPALRGWPATA
                     GVTDEGKRMADETKPTRKGSPPIKVYCLP"
     rep_origin      2547..2853
                     /label=F1 ori
     CDS             4312..4971
                     /codon_start=1
                     /label=AmpR
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      5069..5751
                     /label=ColE1 origin
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagaa ttgtaatacg
      361 actcactata gggcgaatta ttccagctga tcctgctggc ggatactgct attgaagtcc
      421 gattaagcca tgcaagtcta agagtccagc tatgggggct ctggcgtact gctcagtaac
      481 acgtagctaa tctaccctca agaagagcaa aacccgggaa actgggcata atgctctata
      541 agcagaggat actggaatgt tcctttgctt aaatgaagtg caccatgcaa gcactttgct
      601 tgaggatgag gctgcggcgg attaggctgt tggcggggta actgcccacc aaacctttga
      661 tccgtacggg ccttgagaga gggagcccgg agaagggtac tgagataagg accctagccc
      721 tacggggtgc agcaggcgcg aaacctctgc aatgcgcgaa agcgtgacag ggggacttcg
      781 agtgctagct tgctagcttt tgccaagcct aaaaagcttg gcgaataagt gctgggtaag
      841 atgcgtgcca gccgccgcgg taatacgtac agcacaagtg gtatcccctt atattgggcc
      901 taaagcgtgt gtagccggtt ctaatagtct ttggtgaaat caaggggctt aacccattgg
      961 cttgccaaag atactttaaa actcgaggtt ggtagacgtg aagggaattt ctggggtagc
     1021 agtgaaatgt tgtgatccca gaaggaccac cagtagcgaa ggcgcttcac gaggacaagc
     1081 ctgacggtga gacacgaaag ctgagggagc gacccggatt agatacccgg ttagtctcag
     1141 ccgtaaacga tgtggactag gtgtcctagt actcatgtag tacgggggtg ccgaagcgaa
     1201 ggcgctaagt ccaccgcctg ggaagtatac tcgcaagggt gaaacttaat ggaattggcg
     1261 ggggcgcact acaaggggtg gatgctgcgg tttaattgga taaaacgcca gacaactcac
     1321 caggggcgac agtagaatga tggacagtct aaagggcttt cctgacacgc tgagaggtgt
     1381 tgtatggtca tcgtcagctc gtactatgaa gcgtcctgtc aactcaggca acgggcgaga
     1441 cccatgccat tagttgcaat tctgtctttt gacaggagca ctctaatggg actgcagtgg
     1501 caacactgag gaaggaatgg tcaacgatag atctgtatgg cccgaatctc ctgggctaca
     1561 cgcggcatac aatggttgag acaatgagtt cctagcccga aagggtacgg taatccccaa
     1621 aactcaacca cagttcggat cgagggttgt aacccaccct cgtgaagttg gaatccccag
     1681 taatcggaag tcattatctt ccggtgaata agtccctgcg ccttgcacac accgcccatc
     1741 aagccagccg agtagagttc ggatgagcct catttcattg gagtgatgcg attctgagtt
     1801 ttgcgaggcg ggctaagttg taacaaggtg tccgtagggg aacctgcgga cggatcacct
     1861 ccctagcata accccttggg gcctctaaac gggtcttgag gggttttttg tgcatgctcg
     1921 agcggccgcc agtgtgatgg aattcgccct atagtgagtc gtattacaat tcactggccg
     1981 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     2041 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2101 aacagttgcg cagcctgaat ggcgaatgga cgcgctggaa ggcagtacac cttgataggt
     2161 gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg
     2221 ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
     2281 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
     2341 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
     2401 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
     2461 tatgcagcgg aaaagcgctg cttccctgct gttttgtgga atatctaccg actggaaaca
     2521 ggcaaatgca ggaaccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     2581 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     2641 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     2701 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     2761 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2821 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     2881 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2941 aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag ggctgctaaa ggaagcggaa
     3001 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     3061 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     3121 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     3181 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     3241 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg
     3301 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3361 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3421 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3481 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3541 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3601 ggatctcctg tcatcccacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3661 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     3721 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3781 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     3841 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     3901 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     3961 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4021 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4081 tgacgagttc ttctgaattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4141 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4201 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4261 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4321 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4381 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4441 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4501 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4561 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4621 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4681 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4741 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4801 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4861 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4921 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4981 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5041 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5281 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5881 gagcgcagcg agtcagtgag cgaggaagcg gaag
//
