LOCUS       Exported                5975 bp ds-DNA     circular SYN 14-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   HeiAB125
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     source          2002..5975
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=synthetic DNA construct
     misc_binding    177..199
                     /label=LacO
     primer_bind     205..225
                     /label=M13-rev
     misc_feature    350..2001
                     /label=16S_HeiAB125
     primer_bind     complement(2008..2035)
                     /label=T7
     primer_bind     complement(2034..2051)
                     /label=M13-fwd
     CDS             2122..2190
                     /codon_start=1
                     /label=LacZ alpha
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     misc_feature    2175..2215
                     /label=AS1334
     CDS             complement(2196..2595)
                     /codon_start=1
                     /gene="mob"
                     /product="transfer origin"
                     /label=transfer origin
                     /translation="FLHLPVSSR*IFHKTAGKQRFSAA*PCFGVIIAIFSVYPSFFARY
                     TGFCQRVRVDFPWCIQRRQPGRIGEVGPPASGCSFFTVPYSHLAVLNGNPALRGWPATA
                     GVTDEGKRMADETKPTRKGSPPIKVYCLP"
     rep_origin      2608..2914
                     /label=F1 ori
     CDS             4373..5032
                     /codon_start=1
                     /label=AmpR
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      5130..5812
                     /label=ColE1 origin
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagaa ttgtaatacg
      361 actcactata gggcgaatta agccggttga tcatgccgga cctaggtgct atcagattcg
      421 caatagacat gcaagtttaa tgtctcttcg gaggcatggc gaaaggctca gtaacacgtc
      481 gccaatctgc cctatggacg gggataacct cgggaaactg agattaatac ccgataggta
      541 tggaattctg gaaggagcca atacccaaag gggcttcggt tcgccatagg atgaggccgc
      601 ggccgattag ctagtaagtg atgtaaagga tcacttaggc gatgatcggt aggggtaatg
      661 agagttagcg ccccgagaag gacacttaga cactggtcct agcactacgg tgtgcagcag
      721 ttaggaattg tgcccaatgc gcgaaagcgt gagaccgcga atctgagtgc caaggagaac
      781 cttggctgtg atggagtgtt cgtagctcca acagcaagca gagggcaagg ctggtgccag
      841 ccgccgcggt aaaaccagct ctgcgagtgc ctaggacgat tattgggctt aaagcatccg
      901 tagccgggta agtaggtctc tgattaaatc tgcaagctta acttgtaggc tgtcagagat
      961 accgctaacc tagggaatag gagaggtgaa cggtactgcg agagaagcgg tgaaatgcgt
     1021 tgattctcgc aggacccaca gtggcgaagg cggttcactg gaatatctcc gacggtgatg
     1081 gatgaaagcc aggggagcga aagggattag agacccctgt agtcctggcc gtaaacgatg
     1141 aggattaggt gttggtcatg gctaagggcc tggtcagtgc caaagggaaa ctattaaatc
     1201 ctccgcctgg ggagtacggt cgcaaggctg aaacttaaat gaattgacgg gaaagcgcca
     1261 ccaggcacgg gatgtgtggt ttaattcgac tcaacgcgag gaaactcacc tggggcgact
     1321 gttacatgtg tgtcaggctg aagaccttac acgaataaaa cagagaggta gtgcatggcc
     1381 gtctcaagct cgtgccgtga ggtgtgctct taagtgagta aacgagcgag acccgcgccc
     1441 ctatttgcta agagcaagct tcggcttggc tgaggacaat agggggatcg ctatcgatga
     1501 agatagatga aagagcgggc cacggcaggt cagtatgctc caaatcccca gggctacaca
     1561 cgcatcacaa tggacaggac aatgagaagc gactccgaaa ggagaagcgg acctccaaac
     1621 ctgttcgcag tagggatcga ggtctggaac cgacctcgtg aacatggagc gcctagtatc
     1681 cgtgtgtcat catcgcacgg agaatacgtc cccgcttttt gtacacaccg cccgtcgttg
     1741 caacgaaatg aggttcggtt gaggctgagg cgaatcggtt cagtcaaaac tgggcctcgt
     1801 gacgatgcaa aagtcgtaac aaggtatccg taaaattggt gagaggcagt ctcccttttc
     1861 ggtaagggga acctgcggaa atacataagt cgaggcttat gtatcgcaag tggatcacct
     1921 cctctagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gtgcatgctc
     1981 gagcggccgc cagtgtgatg gaattcgccc tatagtgagt cgtattacaa ttcactggcc
     2041 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2101 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2161 caacagttgc gcagcctgaa tggcgaatgg acgcgctgga aggcagtaca ccttgatagg
     2221 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     2281 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     2341 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     2401 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     2461 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     2521 ttatgcagcg gaaaagcgct gcttccctgc tgttttgtgg aatatctacc gactggaaac
     2581 aggcaaatgc aggaaccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2641 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2701 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2761 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2821 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2881 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2941 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3001 caaaaattta acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga
     3061 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     3121 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     3181 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     3241 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     3301 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc
     3361 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3421 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3481 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3541 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3601 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3661 aggatctcct gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     3721 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     3781 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3841 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     3901 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3961 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4021 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4081 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4141 ttgacgagtt cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4201 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4261 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4321 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4381 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4441 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4501 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4561 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4621 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4681 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4741 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4801 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4861 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4921 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4981 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5041 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5101 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5161 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5221 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5281 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5341 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5401 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5461 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5521 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5581 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5641 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5701 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5761 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5821 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5881 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5941 cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
