LOCUS       Exported                3673 bp ds-DNA     circular SYN 24-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 7, 2025 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1
                     /label=start
     promoter        229..235
                     /label=Tet_promoter
     misc_feature    272..1462
                     /label=TetR
     primer_bind     1607..1626
                     /label=pTC14_F2 primer
     misc_feature    1633
                     /label=end
     primer_bind     1703..1724
                     /label=pTC14_R1 primer
     misc_feature    1754..1759
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    1761..1764
                     /label=4nt
                     /note="4nt"
     promoter        1765..2090
                     /label=AtU3 promoter
                     /note="AtU3 promoter"
     modified_base   1840
                     /label=G to c
                     /note="G to c"
     misc_feature    2026..2035
                     /label=USE
                     /note="USE"
     TATA_signal     2060..2067
                     /note="TATA box"
     misc_feature    2091
                     /label=first necletide transcript
                     /note="first necletide transcript"
     misc_recomb     2092..2123
                     /label=Guid RNA cloning site
                     /note="Guid RNA cloning site"
     misc_feature    2124..2199
                     /label=guide RNA scaffold
     primer_bind     2176..2219
                     /label=Primer 1
     misc_feature    2176..2199
                     /label=guide RNA scaffold
                     /note="guide RNA scaffold"
     primer_bind     complement(2176..2193)
                     /label=Primer 2
     primer_bind     2180..2219
                     /label=TSL2-F1
     primer_bind     complement(2180..2199)
                     /label=Linear130-R2
     misc_feature    2200..2245
                     /label=TLS2
     primer_bind     2200..2219
                     /label=TLS2-265E2-F1
     primer_bind     complement(2229..2245)
                     /label=TLS2-265E2-R1
     terminator      2246..2404
                     /note="AtU3 terminator"
     terminator      2380..2420
                     /label=AtU3 terminator
                     /note="AtU3 terminator"
     primer_bind     2380..2404
                     /label=Primer 1
     primer_bind     complement(2380..2404)
                     /label=Primer 2
     misc_feature    2426..2431
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    2496..3169
                     /label=ori
     primer_bind     3565..3584
                     /label=pTC14_F1 primer
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
      241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
      601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
      781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
      841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
     1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1741 tgcctcctct agaggtctcg ccagtttact ttaaattttt cttatggctc agcctgtgat
     1801 ggataactga atcaaacaaa tggcgtctgg gtttaagaac atctgttttg gctatgttgg
     1861 acgaaacaag tgaactttta ggatcaactt ccgtttatat acggagctta tatcgagcaa
     1921 taagataagt gggcttttta tgtaatttaa tgggctatcg tccatatatt cactaatacc
     1981 catgcccagt acccatgtat gcgtttcata taagctccta atttctccca catcgctcaa
     2041 atctaaacaa atcttgttgt atatataaca ctgagggagc accattggtc agagacgaga
     2101 tctagtctga gtcgaccgtc tctgttttag agctagaaat agcaagttaa aataaggcta
     2161 gtccgttatc aacttgaaaa agtggcaccg agtcggtgca ttatcagagt ggtggtgggc
     2221 ccataaccca caggtccgct ctgattccct ttcctttttt cttttttttg ccataaactt
     2281 aaatttgtat atcgatcatt gtagatattg aaaacctaga acaaaccaac atccatgtga
     2341 atgtctttca tgactgattt agagataatt cttgaatttt ggaactagaa tctataatga
     2401 gcctaaatta aaacattgtg tgttggagac cctcgagcca cccatgacca aaatccctta
     2461 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2521 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2581 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2641 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2701 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2761 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2821 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2881 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2941 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3001 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3061 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3121 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3181 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3241 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3301 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3361 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3421 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3481 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3541 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3601 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     3661 ctactctcgc gtt
//
