LOCUS       Exported                3622 bp ds-DNA     circular SYN 24-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 7, 2025 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1
                     /label=start
     promoter        229..235
                     /label=Tet_promoter
     misc_feature    272..1462
                     /label=TetR
     primer_bind     1607..1626
                     /label=pTC14_F2 primer
     misc_feature    1633
                     /label=end
     primer_bind     1703..1724
                     /label=pTC14_R1 primer
     misc_feature    1754..1759
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    1761..1764
                     /label=4nt
                     /note="4nt"
     promoter        1765..2086
                     /label=AtU3 promoter
                     /note="AtU3 promoter"
     modified_base   1840
                     /label=G to c
                     /note="G to c"
     misc_feature    2026..2035
                     /label=USE
                     /note="USE"
     TATA_signal     2060..2067
                     /note="TATA box"
     misc_feature    2092..2111
                     /label=gRNA_ALS
     misc_feature    2112..2194
                     /label=guide RNA scaffold
                     /note="guide RNA scaffold"
     terminator      2195..2369
                     /label=AtU3 terminator
                     /note="AtU3 terminator"
     misc_feature    2375..2380
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    2445..3118
                     /label=ori
     primer_bind     3514..3533
                     /label=pTC14_F1 primer
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
      241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
      601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
      781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
      841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
     1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1741 tgcctcctct agaggtctcc catgtttact ttaaattttt cttatggctc agcctgtgat
     1801 ggataactga atcaaacaaa tggcgtctgg gtttaagaac atctgttttg gctatgttgg
     1861 acgaaacaag tgaactttta ggatcaactt cagtttatat acggagctta tatcgagcaa
     1921 taagataagt gggcttttta tgtaatttaa tgggctatcg tccatatatt cactaatacc
     1981 catgcccagt acccatgtat gcgtttcata taagctccta atttctccca catcgctcaa
     2041 atctaaacaa atcttgttgt atatataaca ctgagggagc accattggtc acaggttccg
     2101 cggcgaatga tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     2161 cttgaaaaag tggcaccgag tcggtgcttt tttttccctt tccttttttc ttttttttgc
     2221 cataaactta aatttgtata tcgatcattg tagatattga aaacctagaa caaaccaaca
     2281 tccatgtgaa tgtctttcat gactgattta gagataattc ttgaattttg gaactagaat
     2341 ctataatgag cctaaattaa aacattgtgg gaccgagacc ctcgagccac ccatgaccaa
     2401 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     2461 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2521 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2581 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2641 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2701 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2761 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2821 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2881 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2941 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3001 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3061 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3121 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3181 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3241 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3301 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     3361 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     3421 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     3481 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     3541 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     3601 ggcagttccc tactctcgcg tt
//
