LOCUS       Exported                3661 bp ds-DNA     circular SYN 24-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 7, 2025 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1
                     /label=start
     promoter        229..235
                     /label=Tet_promoter
     misc_feature    272..1462
                     /label=TetR
     primer_bind     1607..1626
                     /label=pTC14_F2 primer
     misc_feature    1633
                     /label=end
     primer_bind     1703..1724
                     /label=pTC14_R1 primer
     misc_feature    1754..1759
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    1761..1764
                     /label=4nt
                     /note="4nt"
     promoter        1765..2086
                     /label=AtU3 promoter
                     /note="AtU3 promoter"
     modified_base   1840
                     /label=G to c
                     /note="G to c"
     misc_feature    2026..2035
                     /label=USE
                     /note="USE"
     TATA_signal     2060..2067
                     /note="TATA box"
     misc_feature    2092..2111
                     /label=gRNA_ALS
     misc_feature    2112..2187
                     /label=guide RNA scaffold
     primer_bind     2164..2207
                     /label=Primer 1
     misc_feature    2164..2187
                     /label=guide RNA scaffold
                     /note="guide RNA scaffold"
     primer_bind     complement(2164..2181)
                     /label=Linear_130-R3
     primer_bind     complement(2164..2181)
                     /label=Primer 2
     primer_bind     2168..2207
                     /label=TSL2-F1
     primer_bind     complement(2168..2187)
                     /label=Linear_130-R1
     primer_bind     complement(2168..2187)
                     /label=Linear130-R2
     misc_feature    2188..2233
                     /label=TLS2
     primer_bind     2188..2207
                     /label=TLS2-265E2-F1
     primer_bind     complement(2217..2233)
                     /label=TLS2-265E2-R1
     terminator      2234..2392
                     /note="AtU3 terminator"
     terminator      2368..2408
                     /label=AtU3 terminator
                     /note="AtU3 terminator"
     primer_bind     2368..2392
                     /label=Linear_130-F3
     primer_bind     2368..2392
                     /label=Primer 1
     primer_bind     complement(2368..2392)
                     /label=Primer 2
     misc_feature    2414..2419
                     /label=BsaI site
                     /note="BsaI site"
     primer_bind     2449..2468
                     /label=Linear_130-F1
     misc_feature    2484..3157
                     /label=ori
     primer_bind     3553..3572
                     /label=pTC14_F1 primer
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
      241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
      601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
      781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
      841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
     1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1741 tgcctcctct agaggtctcc catgtttact ttaaattttt cttatggctc agcctgtgat
     1801 ggataactga atcaaacaaa tggcgtctgg gtttaagaac atctgttttg gctatgttgg
     1861 acgaaacaag tgaactttta ggatcaactt cagtttatat acggagctta tatcgagcaa
     1921 taagataagt gggcttttta tgtaatttaa tgggctatcg tccatatatt cactaatacc
     1981 catgcccagt acccatgtat gcgtttcata taagctccta atttctccca catcgctcaa
     2041 atctaaacaa atcttgttgt atatataaca ctgagggagc accattggtc acaggttccg
     2101 cggcgaatga tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     2161 cttgaaaaag tggcaccgag tcggtgcatt atcagagtgg tggtgggccc ataacccaca
     2221 ggtccgctct gattcccttt ccttttttct tttttttgcc ataaacttaa atttgtatat
     2281 cgatcattgt agatattgaa aacctagaac aaaccaacat ccatgtgaat gtctttcatg
     2341 actgatttag agataattct tgaattttgg aactagaatc tataatgagc ctaaattaaa
     2401 acattgtggg accgagaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     2461 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2521 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2581 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2641 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2701 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2761 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2821 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2881 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2941 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3001 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3061 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3121 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3181 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3241 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3301 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3361 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3421 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3481 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     3541 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3601 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     3661 t
//
