LOCUS       pAAV_mGAD67(Rii-        5930 bp    DNA     circular SYN 04-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   KonnoA
  TITLE     Direct Submission
  JOURNAL   Exported Aug 18, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5930
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    1..186
                     /label=L-ITR
     primer_bind     177..209
                     /label=#1096 mGAD67(Rii)-XhoI-InF-F
     primer_bind     192..222
                     /label=GAD67(Rii)-InF-F
     misc_feature    193..602
                     /label=mGAD67(Rii-TSS)
     misc_feature    193..461
                     /label=Region ii
     misc_feature    210..214
                     /label=AATTA
     misc_feature    256..260
                     /label=TAATT
     misc_feature    317..320
                     /label=ATTA
     misc_feature    381..384
                     /label=TAAT
     misc_feature    431..602
                     /label=exon1
     misc_feature    431..602
                     /label=TSS
     primer_bind     complement(584..618)
                     /label=GAD67(TSS)-AgeI-InF-R
     primer_bind     complement(584..618)
                     /label=#967 mGAD67(TSS)-AgeI-InF-R
     regulatory      610..619
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    619..1335
                     /label='EGFP
     primer_bind     complement(677..697)
                     /label=#25 GFP-N-R-seq
     misc_feature    1347..1941
                     /label=WPRE
     misc_feature    1961..2090
                     /label='SV40 polyA
     misc_feature    2113..2298
                     /label=ITR
     rep_origin      complement(2781..3294)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3973..4077
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4078..4146,4147..4938)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5109..5697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctagactcg agacaaaatt cttcgtagga attatatttt cccttgccct cacccaacat
      241 cgcctatctc aaaaataatt taaaaacaaa aaacaaacaa acaaacaaaa aacagagcgt
      301 gctgagtgca ttctggatta ctcataggac tttgtcacac acaccctcct ttctggtcgc
      361 aaacccgtga gctggattta taatcgccct acaaagctcc agaggcagtc agacacctgc
      421 aaaggagccc cagcgctccg cggacgagct gcccccgcga gcaacggcct cgtgattccc
      481 cccgccgagc gggtccccgc ctccccactc cgcccccgcc tcccccaagc ccagcggccg
      541 cctctccgga tctctccctt cttcaggctc tcccgtgccg gaccagggat cgtgcaagca
      601 agaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      661 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      721 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      781 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      841 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      901 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      961 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1021 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     1081 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     1141 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1201 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     1261 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1321 gagctgtaca agtaaagcgg ccgcatcgat aatcaacctc tggattacaa aatttgtgaa
     1381 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     1441 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     1501 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     1561 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     1621 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     1681 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     1741 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     1801 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     1861 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     1921 ctttgggccg cctccccgca tcgatgccgt atacggtacc acttgtttat tgcagcttat
     1981 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2041 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tcgaccccca
     2101 acgcgttgga gctctagagc atggctacgt agataagtag catggcgggt taatcattaa
     2161 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     2221 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     2281 cgagcgagcg cgcagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2341 gttgcgcagc ctgaatggcg aatggaantt ccaacatcca ataaatcata caggcaaggc
     2401 aaagaattag caaaattaag caataaagcc tcagagcata aagctaaatc ggttgtacca
     2461 aaaacattat gaccctgtaa tacttttgcg ggagaagcct ttatttcaac gcaaggataa
     2521 aaatttttag aaccctcata tattttaaat gcaatgcctg agtaatgtgt aggtaaagat
     2581 tcaaacgggt gagaaaggcc ggagacagtc aaatcaccat caatatgata ttcaaccgtt
     2641 ctagctgata aattcatgcc ggagagggta gctatttttg agaggtctct acaaaggcta
     2701 tcaggtcatt gcctgagagt ctggagcaaa caagagaatc gatgaacggt aatcgtaaaa
     2761 ctagcatgtc aatcatatgt accccggttg ataatcagaa aagccccaaa aacaggaaga
     2821 ttgtataagc aaatatttaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
     2881 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     2941 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3001 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3061 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3121 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3181 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     3241 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtactatg
     3301 gttgctttga cgagcacgta taacgtgctt tcctcgttag aatcagagcg ggagctaaac
     3361 aggaggccga ttaaagggat tttagacagg aacggtacgc cagaatcctg agaagtgttt
     3421 ttataatcag tgaggccacc gagtaaaaga gtctgtccat cacgcaaatt aaccgttgtc
     3481 gcaatacttc tttgattagt aataacatca cttgcctgag tagaagaact caaactatcg
     3541 gccttgctgg taatatccag aacaatatta ccgccagcca ttgcaacagg aaaaacgctc
     3601 atggaaatac ctacattttg acgctcaatc gtctggaant tcctgatgcg gtattttctc
     3661 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     3721 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3781 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3841 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3901 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3961 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4021 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4081 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4141 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4201 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4261 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4321 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4381 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4441 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4501 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4561 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4621 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4681 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4741 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4801 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4861 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4921 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4981 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5041 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5101 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5161 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5221 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5281 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5341 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5401 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5461 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5521 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5581 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5641 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5701 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5761 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     5821 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     5881 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
//
