LOCUS       Exported                9987 bp ds-DNA     circular SYN 17-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Zhao Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..9987
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     insertion_seq   3108..3125
                     /label=MCS
                     /note="/vntifkey=14"
     regulatory      3144..3153
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3150..4223
                     /label=Gpr45-Del1
     misc_feature    4230..4295
                     /label=3xFlagC
                     /note="/vntifkey=21"
     CDS             4230..4295
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4387..4616
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    4793..5381
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5264..5275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5788..5968
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6000..6224
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6270..6698
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6712..7042
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6893..7028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7090..7137
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7156..7530
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7660..7781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7830..7846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7854..7870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7878..7908)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7923..7944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8232..8820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8991..9851)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9852..9956)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agaattcgga tccgccacca tggcctgtaa cagcacaccc atggggactt
     3181 acgaacatct gctgctgaat gtgagcaaca ctttggaccc tggggacacc ccactgtctg
     3241 caccgctcag gatctcgctg gcaataatga tgctgctgat gatcgtggta ggattccttg
     3301 gcaacacggt ggtctgcatc atcgtgtacc agaggccagc catgcgttca gctatcaacc
     3361 tgctgctggc caccttggcc ttctccgaca tcatgctgtc tttatgctgc atgccattca
     3421 cggccatcac cctcatcact gttcgctggc atttcgggga ccacttttgt cggctttcag
     3481 ctactctcta ttggtttttt gtcctagagg gcgtggccat cctgctcatc attagcgtgg
     3541 accggtttct catcatcgtg cagcgtcagg acaagctgaa cccacgcagg gctaagatga
     3601 tcatcgcggc ctcctgggtg ctgtctttct gcatctctgc gccctccttc actggctgga
     3661 cgttcatgga ggtgcctgct cgagccccac agtgcgtgct aggctacact gagttcccag
     3721 ctgaacgcgc ctatgtagtg acactggtgg tggcagtgtt ctttgctccc ttcggcgtca
     3781 tgttgtgctc ctatctgtgc atcctcaata cggtgctcag acagctgacc ggagctggcc
     3841 tgagacgtct cagacggcag cagcagcagg ccagcctgga cctgagtttc aaaaccaagg
     3901 ccttcaccac catcctcatc ctcttcgtgg gcttttcact ctgctggctg ccacactcag
     3961 tctacagcct gctgtctgcg ttcagccggc ggttctatta cagcgcctcc ttctacacca
     4021 ccagcacatg cgtcctgtgg ctcagttacc tcaagtctgt tttcaacccc atcgtctact
     4081 gctggaggat caagaaattc cgcgaggcct gcatagagtt gcttccccac actttccaaa
     4141 tcctccctaa agtgcctgag cggatccaga ggaaaatcca gccaagcacc atctatgtgt
     4201 gcaacgaaaa ccaatccgct gtcctcgagg actacaaaga ccatgacggt gattataaag
     4261 atcatgacat cgactacaag gatgacgatg acaagtagtg atcgagccat ggaagcttga
     4321 tatctaactg actgaaccgg tggtaccgag gaattaattc gctgtctgcg agggccagct
     4381 gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt
     4441 ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg
     4501 cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg
     4561 agatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     4621 ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt ccgccgatcc
     4681 tggaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     4741 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag acaatcaacc
     4801 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4861 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4921 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4981 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5041 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5101 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5161 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     5221 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5281 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5341 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
     5401 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5461 gggggactgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     5521 taccacacac aaggctactt ccctgattga cagaactaca caccagggcc aggggtcaga
     5581 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taagatagaa
     5641 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     5701 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     5761 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5821 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5881 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5941 ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat cagcctcgac
     6001 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     6061 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6121 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6181 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6241 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6301 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6361 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6421 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6481 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6541 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6601 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt
     6661 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6721 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct
     6781 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6841 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6901 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6961 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     7021 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     7081 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7141 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     7201 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     7261 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     7321 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     7381 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     7441 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     7501 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     7561 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7621 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7681 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7741 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7801 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7861 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7921 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7981 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8041 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8401 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     8461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8641 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9961 tttccccgaa aagtgccacc tgacgtc
//
