LOCUS       Exported                4918 bp ds-DNA     circular SYN 17-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-HA-C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Zhao Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      846..855
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             852..1970
                     /label=Gpr45
     misc_feature    1710..1712
                     /label=Y287C
     misc_feature    1977..2042
                     /label=3xFlagC
                     /note="/vntifkey=21"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattagag
      841 aattcgccac catggcctgt aacagcacac ccatggggac ttacgaacat ctgctgctga
      901 atgtgagcaa cactttggac cctggggaca ccccactgtc tgcaccgctc aggatctcgc
      961 tggcaataat gatgctgctg atgatcgtgg taggattcct tggcaacacg gtggtctgca
     1021 tcatcgtgta ccagaggcca gccatgcgtt cagctatcaa cctgctgctg gccaccttgg
     1081 ccttctccga catcatgctg tctttatgct gcatgccatt cacggccatc accctcatca
     1141 ctgttcgctg gcatttcggg gaccactttt gtcggctttc agctactctc tattggtttt
     1201 ttgtcctaga gggcgtggcc atcctgctca tcattagcgt ggaccggttt ctcatcatcg
     1261 tgcagcgtca ggacaagctg aacccacgca gggctaagat gatcatcgcg gcctcctggg
     1321 tgctgtcttt ctgcatctct gcgccctcct tcactggctg gacgttcatg gaggtgcctg
     1381 ctcgagcccc acagtgcgtg ctaggctaca ctgagttccc agctgaacgc gcctatgtag
     1441 tgacactggt ggtggcagtg ttctttgctc ccttcggcgt catgttgtgc tcctatctgt
     1501 gcatcctcaa tacggtgcgg aagaacgctg tccgtgtgca caaccagtcg gacagcctgg
     1561 acctcagaca gctgaccgga gctggcctga gacgtctcag acggcagcag cagcaggcca
     1621 gcctggacct gagtttcaaa accaaggcct tcaccaccat cctcatcctc ttcgtgggct
     1681 tttcactctg ctggctgcca cactcagtct gcagcctgct gtctgcgttc agccggcggt
     1741 tctattacag cgcctccttc tacaccacca gcacatgcgt cctgtggctc agttacctca
     1801 agtctgtttt caaccccatc gtctactgct ggaggatcaa gaaattccgc gaggcctgca
     1861 tagagttgct tccccacact ttccaaatcc tccctaaagt gcctgagcgg atccagagga
     1921 aaatccagcc aagcaccatc tatgtgtgca acgaaaacca atccgctgtc ctcgaggact
     1981 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
     2041 agtaggcggc cgcggggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     2101 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     2161 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     2221 tcaggttcag ggggaggtgt gggaggtttt ttcggatcct ctagagtcga tctgcaggca
     2281 tgctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2341 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2401 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2461 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2521 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2581 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2701 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2761 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2881 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3121 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3301 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3361 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3421 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3481 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3541 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3601 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3661 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3721 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3781 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3841 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3901 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3961 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4021 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4081 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4141 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4201 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4261 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4321 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4381 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4441 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4501 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     4561 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4621 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     4681 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     4741 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     4801 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     4861 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagt
//
