LOCUS       Exported               12306 bp ds-DNA     circular SYN 12-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..12306
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     misc_feature    3108..6666
                     /label=MCS
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     CDS             3150..6584
                     /label=Adcy3
     CDS             6591..6617
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          6706..6935
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    7112..7700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7583..7594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8107..8287
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8319..8543
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8589..9017
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9031..9361
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9212..9347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9409..9456
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9475..9849
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9979..10100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10149..10165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10173..10189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10197..10227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10242..10263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10551..11139)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11310..12170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12171..12275)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agaattcgga tccgccacca tgccgaggaa ccagggcttc tcggatcccg
     3181 agtactcggc agagtactca gctgaatact cggtcagcct gccctctgac cccgaccgcg
     3241 gggtcggccg gacccatgaa atttctgtgc ggaactcggg gtcctgcctg tgcctgcctc
     3301 gctttatgcg gctgaccttc gtgcctgagt ccttggagaa cctctaccag acctacttca
     3361 aaaggcagcg ccatgagacc ctgctggtgc tggtggtctt tgcggccctc tttgactgct
     3421 acgtggtagt gatgtgcgcg gtggtcttct ccagcgacaa gctggcgccc ctcatggtgg
     3481 caggcttcgg tctggtgttg gacatcatcc ttttcgtgct ctgtaaaaag gggctgctcc
     3541 cggaccgagt gagccgcaaa gtggtaccct acctgctgtg gctgctcatc tcggcccaga
     3601 tcttctccta cctgggcctg aacttttcac gggcccacgc agccagtgac actgtgggtt
     3661 ggcaggcttt ctttgtcttc tccttcttca taacgctgcc cctcagcctc agccccatcg
     3721 tgatcatctc tgtggtctcc tgtgttgtgc acacgcttgt gttgggggtc actgtggccc
     3781 agcagcagca ggatgagctg gaagggatgc agctgctgag ggagatcctg gccaacgtct
     3841 tcctctacct gtgtgctatc atcgtgggca tcatgtccta ctacatggca gaccgcaagc
     3901 accgcaaggc cttcctggag gcccgccagt cactggaggt gaagatgaat ctggaggagc
     3961 agagccagca gcaggaaaac cttatgcttt ccatcctgcc caagcacgtg gctgacgaga
     4021 tgctgaagga catgaagaag gacgagagcc agaaggacca gcagcagttc aataccatgt
     4081 acatgtaccg gcacgagaat gtcagcatcc tgtttgcaga tattgtgggc tttacccagc
     4141 tgtcctctgc ttgcagtgcc caggagctcg tgaagttact caacgagctc ttcgcccgct
     4201 ttgacaagct ggcagccaaa taccaccagc tgaggatcaa gatcctaggc gactgttact
     4261 actgcatctg cggcttgcct gactaccggg aggaccatgc cgtgtgctcc atccttatgg
     4321 ggcttgccat ggtagaggcc atctcgtacg tgcgggagaa gaccaagact ggagtggaca
     4381 tgcgtgtggg agtgcacaca ggcactgtcc taggcggtgt cctgggccag aagcgctggc
     4441 agtatgatgt atggtctact gatgtcaccg tggcaaacaa gatggaggct ggtggcatcc
     4501 cagggcgcgt gcacatttcc cagagcacca tggactgcct gaagggggag tttgatgtgg
     4561 agcctggtga tgggggcagt cgctgcgact acctagatga gaagggcatc gaaacctacc
     4621 tcatcattgc ctccaagcca gaggtgaaga aaacagccca aaatggcctc aacggctcgg
     4681 ccgtgccaaa cggagcgccg gcatcctcca aacccagctc ccctgccctc atcgagacca
     4741 aggagcccaa tggaagtgcc catgccagcg gctccacatc agaggaggct gaagaacagg
     4801 aggcccaggc tgacaacccc tcgttcccca acccccgccg caggctgcgc ctccaggacc
     4861 tggcggaccg tgtggtggac gcctctgagg atgagcacga actgaaccag ctgcttaatg
     4921 aggccctgct ggagcgggag tccgcccagg tggtaaagaa gagaaacaca ttcctcctga
     4981 ccatgaggtt catggaccca gagatggaaa cacgctactc ggtggagaag gagaagcaga
     5041 gcggggctgc cttcagctgc tcctgtgtgg tcctcttctg cacggccatg gtggagatac
     5101 tcattgaccc ctggctgatg acaaactatg tgacctttgt ggttggggag gttctgctcc
     5161 tgatcctgac catctgctcg atggctgcca tctttcccag gtcatttccc aagaagcttg
     5221 tggccttctc atcttggatt gaccggaccc gctgggcaag gaacacctgg gccatgttag
     5281 ccatcttcat tctggttatg gccaatgttg tggacatgct cagctgtctc cagtactaca
     5341 tgggacctta caacatgaca gctgggatgg agctggacgg cggctgcatg gagaacccca
     5401 agtactacaa ctacgtagct gtgctgtcgc tcatcgccac catcatgctg gtgcaggtca
     5461 gccacatggt gaagctgacg ctcatgctgc tcgtcacagg cgccgtgact gccctcaacc
     5521 tgtacgcctg gtgtcctgtc tttgatgaat acgaccacaa gcgctttcag gaaaaggact
     5581 ctcctatggt ggccttagag aagatgcagg tacttgccac ccctgggctc aatggcactg
     5641 acagcaggct gcccctggtg ccttccaagt actccatgac tgtgatgatg ttcgtcatga
     5701 tgctgagctt ttactacttc tcgcgccacg tggaaaaact ggcccgaaca ctgttcttgt
     5761 ggaagattga ggtccatgac cagaaagaac gtgtctacga gatgcgccga tggaacgagg
     5821 ccttggtcac caacatgttg cctgagcatg ttgcacgcca ttttctgggg tccaagaaga
     5881 gagatgagga gctgtacagc cagtcttatg acgagattgg agtcatgttt gcctccttgc
     5941 ccaactttgc tgacttctac actgaggaga gcatcaacaa cggcggcatc gagtgtctac
     6001 gcttcctcaa tgagatcatc tctgattttg actctctcct ggacaatccc aaattccggg
     6061 tcatcaccaa gatcaaaact attggcagca cctatatggc agcttcagga gtcacaccag
     6121 atgtcaacac caatggcttt acaagctcca gcaaggagga gaagtcagac aaggagcgct
     6181 ggcagcacct ggctgacctg gctgactttg cattagccat gaaggacacg ctcacaaaca
     6241 tcaacaacca gtcattcaac aacttcatgt tgcgcatagg catgaacaaa ggaggggttc
     6301 tggctggcgt cattggagcc cggaaaccac actatgacat ctggggcaat acggtcaatg
     6361 tggccagcag gatggaatcc acaggagtca tgggcaacat ccaggtggta gaagagacgc
     6421 aggtcatcct tcgagagtac ggcttccgct ttgtgaggcg aggtcccatc tttgtgaaag
     6481 gcaaagggga gcttctgacc tttttcttga aggggcggga caggccagct gccttcccca
     6541 atggctcctc tgttacactg ccccaccaag tggtggacaa ccccctcgag tacccatacg
     6601 atgttccaga ttacgcttaa tcgagccatg gaagcttgat atctaactga ctgaaccggt
     6661 ggtaccgagg aattaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct
     6721 caaaagcggg catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga
     6781 tattcacctg gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga
     6841 caatcttttt gttgtcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag
     6901 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
     6961 ggtcgagcat gcatctaggg cggccaattc cgccgatcct ggaaaaacat ggagcaatca
     7021 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7081 aggaggtggg ttttccagtc acacctcaga caatcaacct ctggattaca aaatttgtga
     7141 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7201 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7261 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7321 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7381 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7441 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7501 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7561 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7621 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7681 cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac
     7741 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7801 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7861 cctgattgac agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7921 tgctacaagc tagtaccagt tgagccagat aagatagaag aggccaataa aggagagaac
     7981 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     8041 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggac
     8101 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8161 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8221 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8281 tctagcaggg ccgctttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     8341 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     8401 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     8461 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     8521 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     8581 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8641 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     8701 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt
     8761 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     8821 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8881 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     8941 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca
     9001 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     9061 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     9121 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9181 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9241 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9301 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9361 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     9421 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     9481 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     9541 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     9601 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     9661 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     9721 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     9781 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     9841 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     9901 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     9961 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10081 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
    10141 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    10201 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    10261 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    10321 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    10381 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    10441 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    10501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10741 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10981 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    11041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    11101 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    11161 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    11221 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    11281 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    11341 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    11401 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    11461 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    11521 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    11581 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11641 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11701 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11761 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11821 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11881 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11941 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    12001 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    12061 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    12121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    12181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    12241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    12301 gacgtc
//
