LOCUS       Exported               12348 bp ds-DNA     circular SYN 17-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12348)
  AUTHORS   Zhao Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..12348
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     insertion_seq   3108..3125
                     /label=MCS
                     /note="/vntifkey=14"
     misc_feature    3144..6584
                     /label=MCS
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     CDS             3150..6584
                     /label=Adcy3
     misc_feature    6591..6656
                     /label=3xFlagC
                     /note="/vntifkey=21"
     CDS             6591..6656
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          6748..6977
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    7154..7742
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7625..7636)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8149..8329
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8361..8585
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8631..9059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9073..9403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9254..9389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9451..9498
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9517..9891
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10021..10142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10191..10207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10215..10231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10239..10269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10284..10305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10593..11181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11352..12212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12213..12317)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agaattcgga tccgccacca tgccgaggaa ccagggcttc tcggatcccg
     3181 agtactcggc agagtactca gctgaatact cggtcagcct gccctctgac cccgaccgcg
     3241 gggtcggccg gacccatgaa atttctgtgc ggaactcggg gtcctgcctg tgcctgcctc
     3301 gctttatgcg gctgaccttc gtgcctgagt ccttggagaa cctctaccag acctacttca
     3361 aaaggcagcg ccatgagacc ctgctggtgc tggtggtctt tgcggccctc tttgactgct
     3421 acgtggtagt gatgtgcgcg gtggtcttct ccagcgacaa gctggcgccc ctcatggtgg
     3481 caggcttcgg tctggtgttg gacatcatcc ttttcgtgct ctgtaaaaag gggctgctcc
     3541 cggaccgagt gagccgcaaa gtggtaccct acctgctgtg gctgctcatc tcggcccaga
     3601 tcttctccta cctgggcctg aacttttcac gggcccacgc agccagtgac actgtgggtt
     3661 ggcaggcttt ctttgtcttc tccttcttca taacgctgcc cctcagcctc agccccatcg
     3721 tgatcatctc tgtggtctcc tgtgttgtgc acacgcttgt gttgggggtc actgtggccc
     3781 agcagcagca ggatgagctg gaagggatgc agctgctgag ggagatcctg gccaacgtct
     3841 tcctctacct gtgtgctatc atcgtgggca tcatgtccta ctacatggca gaccgcaagc
     3901 accgcaaggc cttcctggag gcccgccagt cactggaggt gaagatgaat ctggaggagc
     3961 agagccagca gcaggaaaac cttatgcttt ccatcctgcc caagcacgtg gctgacgaga
     4021 tgctgaagga catgaagaag gacgagagcc agaaggacca gcagcagttc aataccatgt
     4081 acatgtaccg gcacgagaat gtcagcatcc tgtttgcaga tattgtgggc tttacccagc
     4141 tgtcctctgc ttgcagtgcc caggagctcg tgaagttact caacgagctc ttcgcccgct
     4201 ttgacaagct ggcagccaaa taccaccagc tgaggatcaa gatcctaggc gactgttact
     4261 actgcatctg cggcttgcct gactaccggg aggaccatgc cgtgtgctcc atccttatgg
     4321 ggcttgccat ggtagaggcc atctcgtacg tgcgggagaa gaccaagact ggagtggaca
     4381 tgcgtgtggg agtgcacaca ggcactgtcc taggcggtgt cctgggccag aagcgctggc
     4441 agtatgatgt atggtctact gatgtcaccg tggcaaacaa gatggaggct ggtggcatcc
     4501 cagggcgcgt gcacatttcc cagagcacca tggactgcct gaagggggag tttgatgtgg
     4561 agcctggtga tgggggcagt cgctgcgact acctagatga gaagggcatc gaaacctacc
     4621 tcatcattgc ctccaagcca gaggtgaaga aaacagccca aaatggcctc aacggctcgg
     4681 ccgtgccaaa cggagcgccg gcatcctcca aacccagctc ccctgccctc atcgagacca
     4741 aggagcccaa tggaagtgcc catgccagcg gctccacatc agaggaggct gaagaacagg
     4801 aggcccaggc tgacaacccc tcgttcccca acccccgccg caggctgcgc ctccaggacc
     4861 tggcggaccg tgtggtggac gcctctgagg atgagcacga actgaaccag ctgcttaatg
     4921 aggccctgct ggagcgggag tccgcccagg tggtaaagaa gagaaacaca ttcctcctga
     4981 ccatgaggtt catggaccca gagatggaaa cacgctactc ggtggagaag gagaagcaga
     5041 gcggggctgc cttcagctgc tcctgtgtgg tcctcttctg cacggccatg gtggagatac
     5101 tcattgaccc ctggctgatg acaaactatg tgacctttgt ggttggggag gttctgctcc
     5161 tgatcctgac catctgctcg atggctgcca tctttcccag gtcatttccc aagaagcttg
     5221 tggccttctc atcttggatt gaccggaccc gctgggcaag gaacacctgg gccatgttag
     5281 ccatcttcat tctggttatg gccaatgttg tggacatgct cagctgtctc cagtactaca
     5341 tgggacctta caacatgaca gctgggatgg agctggacgg cggctgcatg gagaacccca
     5401 agtactacaa ctacgtagct gtgctgtcgc tcatcgccac catcatgctg gtgcaggtca
     5461 gccacatggt gaagctgacg ctcatgctgc tcgtcacagg cgccgtgact gccctcaacc
     5521 tgtacgcctg gtgtcctgtc tttgatgaat acgaccacaa gcgctttcag gaaaaggact
     5581 ctcctatggt ggccttagag aagatgcagg tacttgccac ccctgggctc aatggcactg
     5641 acagcaggct gcccctggtg ccttccaagt actccatgac tgtgatgatg ttcgtcatga
     5701 tgctgagctt ttactacttc tcgcgccacg tggaaaaact ggcccgaaca ctgttcttgt
     5761 ggaagattga ggtccatgac cagaaagaac gtgtctacga gatgcgccga tggaacgagg
     5821 ccttggtcac caacatgttg cctgagcatg ttgcacgcca ttttctgggg tccaagaaga
     5881 gagatgagga gctgtacagc cagtcttatg acgagattgg agtcatgttt gcctccttgc
     5941 ccaactttgc tgacttctac actgaggaga gcatcaacaa cggcggcatc gagtgtctac
     6001 gcttcctcaa tgagatcatc tctgattttg actctctcct ggacaatccc aaattccggg
     6061 tcatcaccaa gatcaaaact attggcagca cctatatggc agcttcagga gtcacaccag
     6121 atgtcaacac caatggcttt acaagctcca gcaaggagga gaagtcagac aaggagcgct
     6181 ggcagcacct ggctgacctg gctgactttg cattagccat gaaggacacg ctcacaaaca
     6241 tcaacaacca gtcattcaac aacttcatgt tgcgcatagg catgaacaaa ggaggggttc
     6301 tggctggcgt cattggagcc cggaaaccac actatgacat ctggggcaat acggtcaatg
     6361 tggccagcag gatggaatcc acaggagtca tgggcaacat ccaggtggta gaagagacgc
     6421 aggtcatcct tcgagagtac ggcttccgct ttgtgaggcg aggtcccatc tttgtgaaag
     6481 gcaaagggga gcttctgacc tttttcttga aggggcggga caggccagct gccttcccca
     6541 atggctcctc tgttacactg ccccaccaag tggtggacaa ccccctcgag gactacaaag
     6601 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt
     6661 gatcgagcca tggaagcttg atatctaact gactgaaccg gtggtaccga ggaattaatt
     6721 cgctgtctgc gagggccagc tgttggggtg agtactccct ctcaaaagcg ggcatgactt
     6781 ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg
     6841 tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa
     6901 gcttgaggtg tggcaggctt gagatctggc catacacttg agtgacaatg acatccactt
     6961 tgcctttctc tccacaggtg tccactccca ggtccaactg caggtcgagc atgcatctag
     7021 ggcggccaat tccgccgatc ctggaaaaac atggagcaat cacaagtagc aatacagcag
     7081 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7141 tcacacctca gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7201 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7261 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7321 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7381 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7441 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7501 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7561 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7621 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7681 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7741 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7801 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca aagaagacaa
     7861 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg acagaactac
     7921 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     7981 gttgagccag ataagataga agaggccaat aaaggagaga acaccagctt gttacaccct
     8041 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     8101 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     8161 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8221 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8281 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggccgcttta
     8341 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     8401 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     8461 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     8521 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     8581 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     8641 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8701 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8761 ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg
     8821 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8881 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8941 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9001 ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     9061 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag
     9121 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     9181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     9241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     9361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     9421 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     9481 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     9541 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     9601 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     9661 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     9721 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     9781 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     9841 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     9901 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     9961 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    10021 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10081 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10141 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
    10201 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    10261 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    10321 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    10381 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10501 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    10561 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    10621 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10681 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10741 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
    10801 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10861 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10921 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10981 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    11041 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    11101 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    11161 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    11221 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    11281 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    11341 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    11401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    11461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    11521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    11581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    11641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    11701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    12001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    12061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    12121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    12181 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    12241 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    12301 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
