LOCUS       Exported               10554 bp ds-DNA     circular SYN 13-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     regulatory      3144..3153
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    3150..3770
                     /label=Mrap2
     CDS             3780..3836
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3837..4832
                     /label=Mc4r
     CDS             4839..4865
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4954..5183
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    5360..5948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5831..5842)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6355..6535
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6567..6791
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6837..7265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7279..7609
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7460..7595
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7657..7704
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7723..8097
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8227..8348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8397..8413)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8421..8437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8445..8475)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8490..8511
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8799..9387)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9558..10418)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10419..10523)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agaattcgga tccgccacca tggagatgtc tgcccagagg ctggcttcta
     3181 acaggacatc cccacagtca ccatcgaact ctgactacac ctgggaatac gagtactatg
     3241 agatcggacc agtttccttt gaaggactga aggctcataa atattccatt gtgattggat
     3301 tctgggttgg tcttgcggtc tttgtgattt tcatgttttt tgtgctgact ttgctgacga
     3361 agacaggtgc cccacaccaa gacaacgcgg agtcctcaga gaggaggttc cgcatgaaca
     3421 gctttgtgtc agacttcggg aagccgctgg agtcagacaa ggtgttttct cgtcagggca
     3481 acgaggagtc caggtctctg ttccactgtt acatcaatga agtagaacac ttggacaggg
     3541 ttaaagtttg ccaccaaaca acagccatcg acagtgatgt ccacctccag gaagccagca
     3601 gaagcagtgg gaggcctgag gaggagctag ccaggttcat gaagtttgac atccccaact
     3661 ttgtgaacac agagcagagc tcctttgggg aggatgatct cctgatttcg gaagcacccg
     3721 ttcttctaga aaacaagcca gtttcccaga catcacgcat agacctggac ggcagcggcg
     3781 ccaccaactt cagcctgctg aagcaggccg gcgacgtgga ggagaacccc ggccccatga
     3841 actccaccca ccaccatggc atgtatactt ccctccacct ctggaaccgc agcagctacg
     3901 ggctgcacgg caatgccagc gagtcgctgg ggaagggcca cccggacgga ggatgctatg
     3961 agcaactttt tgtttccccc gaggtgtttg tgactctggg tgtcataagc ctgttggaga
     4021 acattctagt gatcgtggcg atagccaaga acaagaacct gcactcaccc atgtactttt
     4081 tcatctgtag cctggctgtg gcagatatgc tggtgagcgt ttcgaatggg tcggaaacca
     4141 tcgtcattac cctgttaaac agtacggata cggatgccca gagcttcacc gtgaacattg
     4201 ataatgtcat tgactctgtg atctgtagct ccttgctcgc atccatttgc agcctgcttt
     4261 ccattgcggt ggacaggtat ttcactatct tttacgcgct ccagtaccat aacatcatga
     4321 cggttaggcg ggtcgggatc atcataagtt gtatctgggc agcttgcact gtgtcaggcg
     4381 tcctcttcat catttactcg gacagcagcg ctgtcatcat ctgcctcatt tccatgttct
     4441 tcactatgct agttctcatg gcctctctct atgtccacat gttcctgatg gcgaggcttc
     4501 acattaagag gattgctgtc ctcccaggca cagggaccat ccgccagggt accaacatga
     4561 agggggcgat taccttgacc atcctgattg gagtctttgt tgtctgctgg gccccgttct
     4621 ttctccattt actgttctac atctcttgcc ctcagaatcc atactgcgtg tgcttcatgt
     4681 ctcattttaa tttgtatctc atactgatca tgtgtaacgc cgtcatcgac cctctcattt
     4741 atgccctccg gagtcaagaa ctgaggaaaa ctttcaaaga gatcatctgt ttctatcctc
     4801 tgggaggcat ctgtgagttg tctagcaggt atctcgagta cccatacgat gttccagatt
     4861 acgcttaatc gagccatgga agcttgatat ctaactgact gaaccggtgg taccgaggaa
     4921 ttaattcgct gtctgcgagg gccagctgtt ggggtgagta ctccctctca aaagcgggca
     4981 tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc
     5041 ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc agaaaagaca atctttttgt
     5101 tgtcaagctt gaggtgtggc aggcttgaga tctggccata cacttgagtg acaatgacat
     5161 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcagg tcgagcatgc
     5221 atctagggcg gccaattccg ccgatcctgg aaaaacatgg agcaatcaca agtagcaata
     5281 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     5341 ttccagtcac acctcagaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5401 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5461 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5521 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5581 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5641 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5701 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     5761 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5821 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5881 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5941 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     6001 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga
     6061 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattgacag
     6121 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     6181 gtaccagttg agccagataa gatagaagag gccaataaag gagagaacac cagcttgtta
     6241 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     6301 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggactg tactgggtct
     6361 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6421 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6481 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     6541 gctttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6601 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6661 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6721 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6781 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     6841 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6901 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6961 cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc
     7021 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7081 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7141 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7201 gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7261 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc
     7321 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7381 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7441 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7501 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     7561 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     7621 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     7681 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     7741 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     7801 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     7861 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     7921 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     7981 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     8041 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     8101 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8161 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8221 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8281 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8341 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8401 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8461 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8521 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8581 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     9001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     9241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9661 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9841 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10141 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
