LOCUS       Exported               10587 bp ds-DNA     circular SYN 12-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Zhao Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10587
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     insertion_seq   3108..3125
                     /label=MCS
                     /note="/vntifkey=14"
     regulatory      3144..3153
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3150..4823
                     /label=Gpr161
     misc_feature    4830..4895
                     /label=3xFlagC
                     /note="/vntifkey=21"
     CDS             4830..4895
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4987..5216
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    5393..5981
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5864..5875)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6388..6568
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6600..6824
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6870..7298
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7312..7642
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7493..7628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7690..7737
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7756..8130
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8260..8381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8430..8446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8454..8470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8478..8508)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8523..8544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8832..9420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9591..10451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10452..10556)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agaattcgga tccgccacca tggactcgca ccacaccaca cacactctct
     3181 tggctgtatt tcctgtcgtc cagcatgctc tgctcaccgc atcccgaggg gcactgacca
     3241 tgagcctcaa ctcctccctc agctacagga aggaactgag caacctcact gcaacagagg
     3301 gtggtgaagg gggtgccgtc tctgagttca tcgccatcat catcatcact gtcctcgtct
     3361 gcctgggcaa cctggtcatt gtagtcacct tgtacaagaa gtcctacctc ctcaccctca
     3421 gcaacaagtt cgtcttcagc ctgaccttgt ccaacttcct gttgtcggtg ctggtgctgc
     3481 cgttcgtggt gactagctcc atccggaggg aatggatttt cggcgtggtt tggtgcaact
     3541 tctctgccct cctctacctg ctgatcagct cagccagcat gctcacgctt ggggtcattg
     3601 ccatcgatcg ctactacgcc gtcctgtatc caatggtgta ccccatgaag atcacaggga
     3661 accgagctgt gatggctctc gtctacatct ggctccactc tctcattggc tgtctgccgc
     3721 ccctatttgg ttggtcatcg gtggagtttg atgagttcaa gtggatgtgt gtggccgcgt
     3781 ggcaccagga acccggctac accattttct ggcagatctg gtgtgccctg ttcccctttc
     3841 tcatcatgct agtgtgctac ggtttcatct tccgggtggc cagggtcaag gcccgaaagg
     3901 tgcactgtgg cacggtggtc actgtggagg aggactctca gaggagcggg aggaagaatt
     3961 ctagtacctc cacttcctcc tccggcagta ggaggaatgc ccttcaagga gtggtctatt
     4021 cagctaacca gtgcaaagcc ctcatcacca tcctggtggt cattggcgcc ttcatggtca
     4081 cctggggccc ctacatggtt gtcattacct cagaggcact ctgggggaag aactgtgtct
     4141 ccccaaccct ggagacttgg gccacatggc tgtcctttac cagtgccatc tgccaccctc
     4201 tgatctacgg actctggaac aagactgtgc gcaaggagct cctgggcatg tgctttgggg
     4261 accgttatta ccgggaatcc tttgtgcagc gacagaggac ctccaggctc ttcagcattt
     4321 ccaacaggat cacagacttg ggtctgtccc cacatctcac ggcgctcatg gcaggcggac
     4381 agtccctggg acacagcagc agcaccggtg acacaggctt cagctactct caggattcag
     4441 ggacggatgt gatgctgttg gaagatggca cttctgagga caaccctccc cagcactgca
     4501 cttgcccacc caagaggagg agctccgtga catttgagga cgaagtggaa cagatcaaag
     4561 aggccgccaa gaactctctt cttcatgtga aagctgaagt acacaaatcc ttggacagtt
     4621 acgcagccag cttggctaaa gccattgagg ctgaagccaa aatcaactta tttggggagg
     4681 aggctctgcc aggggtcttg ttcacagcac ggaccgtccc aggggctggc tttgggggcc
     4741 gtcgaggcag cagaactctt gtgaatcaga ggctgcagct acagagcatc aaagaaggca
     4801 atgtcttagc tgcagaacag agactcgagg actacaaaga ccatgacggt gattataaag
     4861 atcatgacat cgactacaag gatgacgatg acaagtagtg atcgagccat ggaagcttga
     4921 tatctaactg actgaaccgg tggtaccgag gaattaattc gctgtctgcg agggccagct
     4981 gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt
     5041 ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg
     5101 cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg
     5161 agatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     5221 ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt ccgccgatcc
     5281 tggaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     5341 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag acaatcaacc
     5401 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5461 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5521 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5581 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5641 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5701 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5761 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     5821 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5881 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5941 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
     6001 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6061 gggggactgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     6121 taccacacac aaggctactt ccctgattga cagaactaca caccagggcc aggggtcaga
     6181 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taagatagaa
     6241 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     6301 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     6361 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6421 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6481 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6541 ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat cagcctcgac
     6601 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     6661 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6721 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6781 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6841 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6901 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6961 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7021 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7081 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7141 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7201 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt
     7261 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     7321 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct
     7381 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7441 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7501 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7561 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     7621 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     7681 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7741 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     7801 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     7861 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     7921 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     7981 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     8041 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     8101 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     8161 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8221 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     8281 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8341 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     8401 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8461 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8521 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8581 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8641 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8701 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8761 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8821 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8881 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8941 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9001 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     9061 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9121 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9181 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9241 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     9301 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9361 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9421 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9481 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9541 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9601 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9661 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9721 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9781 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9841 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9901 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9961 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10021 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10081 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10141 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10201 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10261 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10321 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10381 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10441 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10501 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10561 tttccccgaa aagtgccacc tgacgtc
//
