LOCUS       Exported               10500 bp ds-DNA     circular SYN 12-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Zhao Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     insertion_seq   3108..3125
                     /label=MCS
                     /note="/vntifkey=14"
     CDS             3150..4130
                     /label=Gpr45-N
     misc_feature    4131..4736
                     /label=Gpr161-C
     misc_feature    4743..4808
                     /label=3xFlagC
                     /note="/vntifkey=21"
     CDS             4743..4808
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4900..5129
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    5306..5894
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5777..5788)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6301..6481
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6513..6737
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6783..7211
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7225..7555
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7406..7541
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7603..7650
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7669..8043
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8173..8294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8343..8359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8367..8383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8391..8421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8436..8457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8745..9333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9504..10364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10365..10469)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agaattcgga tccgccacca tggcctgtaa cagcacaccc atggggactt
     3181 acgaacatct gctgctgaat gtgagcaaca ctttggaccc tggggacacc ccactgtctg
     3241 caccgctcag gatctcgctg gcaataatga tgctgctgat gatcgtggta ggattccttg
     3301 gcaacacggt ggtctgcatc atcgtgtacc agaggccagc catgcgttca gctatcaacc
     3361 tgctgctggc caccttggcc ttctccgaca tcatgctgtc tttatgctgc atgccattca
     3421 cggccatcac cctcatcact gttcgctggc atttcgggga ccacttttgt cggctttcag
     3481 ctactctcta ttggtttttt gtcctagagg gcgtggccat cctgctcatc attagcgtgg
     3541 accggtttct catcatcgtg cagcgtcagg acaagctgaa cccacgcagg gctaagatga
     3601 tcatcgcggc ctcctgggtg ctgtctttct gcatctctgc gccctccttc actggctgga
     3661 cgttcatgga ggtgcctgct cgagccccac agtgcgtgct aggctacact gagttcccag
     3721 ctgaacgcgc ctatgtagtg acactggtgg tggcagtgtt ctttgctccc ttcggcgtca
     3781 tgttgtgctc ctatctgtgc atcctcaata cggtgcggaa gaacgctgtc cgtgtgcaca
     3841 accagtcgga cagcctggac ctcagacagc tgaccggagc tggcctgaga cgtctcagac
     3901 ggcagcagca gcaggccagc ctggacctga gtttcaaaac caaggccttc accaccatcc
     3961 tcatcctctt cgtgggcttt tcactctgct ggctgccaca ctcagtctac agcctgctgt
     4021 ctgcgttcag ccggcggttc tattacagcg cctccttcta caccaccagc acatgcgtcc
     4081 tgtggctcag ttacctcaag tctgttttca accccatcgt ctactgctgg aacaagactg
     4141 tgcgcaagga gctcctgggc atgtgctttg gggaccgtta ttaccgggaa tcctttgtgc
     4201 agcgacagag gacctccagg ctcttcagca tttccaacag gatcacagac ttgggtctgt
     4261 ccccacatct cacggcgctc atggcaggcg gacagtccct gggacacagc agcagcaccg
     4321 gtgacacagg cttcagctac tctcaggatt cagggacgga tgtgatgctg ttggaagatg
     4381 gcacttctga ggacaaccct ccccagcact gcacttgccc acccaagagg aggagctccg
     4441 tgacatttga ggacgaagtg gaacagatca aagaggccgc caagaactct cttcttcatg
     4501 tgaaagctga agtacacaaa tccttggaca gttacgcagc cagcttggct aaagccattg
     4561 aggctgaagc caaaatcaac ttatttgggg aggaggctct gccaggggtc ttgttcacag
     4621 cacggaccgt cccaggggct ggctttgggg gccgtcgagg cagcagaact cttgtgaatc
     4681 agaggctgca gctacagagc atcaaagaag gcaatgtctt agctgcagaa cagagactcg
     4741 aggactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
     4801 atgacaagta gtgatcgagc catggaagct tgatatctaa ctgactgaac cggtggtacc
     4861 gaggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc ctctcaaaag
     4921 cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
     4981 cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa aagacaatct
     5041 ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact tgagtgacaa
     5101 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcaggtcga
     5161 gcatgcatct agggcggcca attccgccga tcctggaaaa acatggagca atcacaagta
     5221 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5281 tgggttttcc agtcacacct cagacaatca acctctggat tacaaaattt gtgaaagatt
     5341 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5401 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5461 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5521 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5581 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5641 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5701 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5761 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5821 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5881 ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     5941 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6001 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     6061 tgacagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     6121 aagctagtac cagttgagcc agataagata gaagaggcca ataaaggaga gaacaccagc
     6181 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     6241 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggactgtact
     6301 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6361 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6421 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6481 agggccgctt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6541 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6601 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6661 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6721 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     6781 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6841 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6901 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt
     6961 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7021 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7081 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7141 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7201 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7261 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7321 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7381 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7441 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7501 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7561 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     7621 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     7681 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     7741 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     7801 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     7861 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     7921 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     7981 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     8041 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8101 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8161 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8281 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8341 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8401 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8461 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     8941 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9181 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9541 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9601 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9661 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9721 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9781 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9841 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9901 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9961 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10021 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10081 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10141 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10201 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10261 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10321 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10381 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10441 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
