LOCUS       Exported               10219 bp ds-DNA     circular SYN 17-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBOB-3xFlag-N
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Zhao Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     misc_feature    3108..4579
                     /label=MCS
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     CDS             3141..3167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3186..4532
                     /label=Gnal
     intron          4619..4848
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    5025..5613
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5496..5507)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6020..6200
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6232..6456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6502..6930
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6944..7274
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7125..7260
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7322..7369
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7388..7762
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7892..8013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8062..8078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8086..8102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8110..8140)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8155..8176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8464..9052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9223..10083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10084..10188)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agccaccatg tacccatacg atgttccaga ttacgcttct agagaattcg
     3181 gatccatggg cctatgctac agcctgcggc cgctgctctt cgggagccca gaggacaccc
     3241 cgtgtgcggc ctcggaaccc tgcgcagagg atgctcagcc cagcgccgcc ccggcccctg
     3301 cctcgatccc agccccggct cccgtaggga ccctgctccg gcgtggcggc ggccggatcg
     3361 tcgcgaacgc gcggccgcca ggcgagctgc agagccgccg gcgacaggag cagctacgag
     3421 ccgaggagcg cgaggcggct aaagaggcga ggaaagtcag ccggggcatc gaccgcatgc
     3481 tgcgcgagca gaagcgggac ctgcagcaga cgcaccggct cctgctgctg ggggctggtg
     3541 agtccgggaa aagcactatc gtcaaacaga tgaggatcct gcacgtcaat ggcttcaacc
     3601 ccgaggaaaa gaagcagaaa attctggaca tcaggaaaaa tgtcaaagat gcgatcgtga
     3661 caatcgtttc agcaatgagt actatcatac ctccagttcc actggccaac cctgagaacc
     3721 agttccggtc agattatatc aagagcatag cccctatcac tgactttgaa tattcccagg
     3781 agttctttga ccatgtgaag aagctgtggg acgatgaagg agtgaaggcc tgctttgaga
     3841 gatccaacga gtaccagctg atcgactgtg cacaatactt cctggaaagg attgacagtg
     3901 tcagtctggt tgactacaca cccacagacc aggacctgct cagatgcaga gtgctgacat
     3961 caggaatctt tgagacacga ttccaagtgg acaaagtgaa ctttcacatg tttgatgttg
     4021 gaggccagag agatgagaga agaaaatgga tccagtgttt taatgatgtc actgcgatca
     4081 tttacgtggc ggcctgtagt agctacaaca tggtgatccg ggaagataac aataccaaca
     4141 gacttcggga atcactggac ctgtttgaaa gcatctggaa taacaggtgg ttgcgaacca
     4201 tttctatcat cctattcttg aacaaacaag acatgctggc agaaaaagtc ttggcaggga
     4261 agtcaaaaat cgaagactat ttcccggagt atgccaatta tactgtccct gaagatgcaa
     4321 caccagatgc gggagaagat cccaaagtta caagagcaaa gttctttatc cgggatctgt
     4381 tcttgaggat cagcacagcc acgggtgatg gcaaacatta ctgctaccct cacttcacct
     4441 gcgccgtgga cacagagaac atccgcagag tgttcaacga ttgccgtgac atcatccaga
     4501 gaatgcatct caagcagtac gaactcttgt gactcgagcc atggaagctt gatatctaac
     4561 tgactgaacc ggtggtaccg aggaattaat tcgctgtctg cgagggccag ctgttggggt
     4621 gagtactccc tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac
     4681 gaggaggatt tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc
     4741 tggtcagaaa agacaatctt tttgttgtca agcttgaggt gtggcaggct tgagatctgg
     4801 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc
     4861 aggtccaact gcaggtcgag catgcatcta gggcggccaa ttccgccgat cctggaaaaa
     4921 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     4981 gcacaagagg aggaggaggt gggttttcca gtcacacctc agacaatcaa cctctggatt
     5041 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5101 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5161 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5221 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5281 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5341 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5401 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     5461 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5521 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5581 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag
     5641 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5701 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     5761 acaaggctac ttccctgatt gacagaacta cacaccaggg ccaggggtca gatatccact
     5821 gacctttgga tggtgctaca agctagtacc agttgagcca gataagatag aagaggccaa
     5881 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     5941 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     6001 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6061 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6121 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6181 agtgtggaaa atctctagca gggccgcttt aaacccgctg atcagcctcg actgtgcctt
     6241 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6301 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6361 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6421 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6481 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6541 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6601 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     6661 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6721 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6781 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6841 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     6901 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     6961 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     7021 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7081 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7141 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7201 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7261 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     7321 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     7381 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     7441 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     7501 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     7561 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     7621 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     7681 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     7741 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     7801 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7861 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7921 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7981 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8041 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8101 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8161 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8221 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8641 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8881 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9241 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9301 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9361 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9421 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9481 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9541 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9601 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9661 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9721 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9781 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9841 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9901 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9961 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10021 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10081 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10141 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10201 aaaagtgcca cctgacgtc
//
