LOCUS       Exported                9937 bp ds-DNA     circular SYN 17-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBOB-3xFlag-N
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Zhao Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     misc_feature    3108..4297
                     /label=MCS
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     CDS             3141..3167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3186..4250
                     /label=Gnao1-A
     intron          4337..4566
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    4743..5331
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5214..5225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5738..5918
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5950..6174
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6220..6648
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6662..6992
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6843..6978
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7040..7087
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7106..7480
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7610..7731
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7780..7796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7804..7820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7828..7858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7873..7894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8182..8770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8941..9801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9802..9906)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agccaccatg tacccatacg atgttccaga ttacgcttct agagaattcg
     3181 gatccatggg atgtacgctg agcgcagagg agagagccgc cctcgagcgg agcaaggcga
     3241 ttgagaaaaa cctcaaagaa gatggcatca gcgccgccaa agacgtgaaa ttactcctgc
     3301 tgggggctgg agaatcagga aaaagcacca ttgtgaagca gatgaagatc atccatgaag
     3361 atggcttctc tggggaagac gtgaagcagt acaagcctgt ggtctacagc aacaccatcc
     3421 agtctctggc ggccattgtc cgggccatgg acactttggg cgtggagtat ggtgacaagg
     3481 agaggaagac ggactccaag atggtgtgtg acgtggtgag tcgtatggaa gacactgaac
     3541 cgttctctgc agaacttctt tctgccatga tgcgactctg gggcgactcg gggatccagg
     3601 agtgcttcaa ccgatctcgg gagtatcagc tcaatgactc tgccaaatac tacctggaca
     3661 gcctggatcg gattggagcc ggtgactacc agcccactga gcaggacatc ctccgaacca
     3721 gagtcaaaac aactggcatc gtagaaaccc acttcacctt caagaacctc cacttcaggc
     3781 tgtttgacgt cgggggccag cgatctgaac gcaagaagtg gatccactgc tttgaggatg
     3841 tcacggccat catcttctgt gtcgcactca gcggctatga ccaggtgctc cacgaggacg
     3901 aaaccacgaa ccgcatgcac gagtctctca tgctcttcga ctccatctgt aacaacaagt
     3961 ttttcattga tacctccatc atcctcttcc tcaacaagaa agacctcttt ggcgagaaga
     4021 ttaagaagtc acccttgacc atctgctttc ccgaataccc aggctccaac acctatgaag
     4081 atgcagctgc ctacatccaa acacagtttg aaagcaaaaa ccgctcaccc aacaaagaaa
     4141 tttactgtca catgacttgt gccacagaca cgaataatat ccaggtggta ttcgacgccg
     4201 tcaccgacat catcattgcc aacaatctcc ggggctgcgg cttgtactga ctcgagccat
     4261 ggaagcttga tatctaactg actgaaccgg tggtaccgag gaattaattc gctgtctgcg
     4321 agggccagct gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga
     4381 ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg
     4441 agggtggccg cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt
     4501 ggcaggcttg agatctggcc atacacttga gtgacaatga catccacttt gcctttctct
     4561 ccacaggtgt ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt
     4621 ccgccgatcc tggaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4681 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     4741 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4801 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4861 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4921 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4981 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5041 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5101 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     5161 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5221 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5281 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     5341 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5401 aaaagaaaag gggggactgg aagggctaat tcactcccaa agaagacaag atatccttga
     5461 tctgtggatc taccacacac aaggctactt ccctgattga cagaactaca caccagggcc
     5521 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     5581 taagatagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     5641 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     5701 tcatcacgtg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     5761 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5821 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5881 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat
     5941 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6001 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6061 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6121 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6181 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     6241 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6301 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6361 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6421 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6481 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6541 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg
     6601 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     6661 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa
     6721 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6781 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6841 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6901 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6961 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7021 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7081 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     7141 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7201 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     7261 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     7321 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     7381 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     7441 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     7501 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7561 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7621 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7681 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7741 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7801 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7861 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7921 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7981 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8341 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     8401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8581 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8821 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8881 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8941 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9001 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9061 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9121 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9181 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9241 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9301 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9361 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9421 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9481 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9541 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9601 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9661 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9721 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9781 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9841 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9901 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
