LOCUS       snuABE_3.1dna           9690 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Molecular Genome Engineering Laboratory
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
     CDS             409..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             466..1713
                     /codon_start=1
                     /label=P. humanus ADARdd
                     /note="color: #ccffff"
                     /translation="PSYSPVKMNPLACLDAKMESVPQIFADHVATLINKKFGELIKNNP
                     AYSRRRVLAGIVMSEDYNLESCKVLTVSTGTKCISGDRISANGSVLNDSHAEIISRRCL
                     CNYFYSELEKYVKDDTGRNSIFLKSPNKGFLIDPKYKFHLYITSAPCGDSRLFSPHDVC
                     IFDKHPNRKARGQLRTKIERGQGTIPVNSSDLIQTWDGVLVGERLLTMSCSDKLAKWNV
                     VGVQGALLSNFIQPVYLDSIILGSLFHPSHLYRAVYGRIENEIYDLTPPYRLNKPKLGA
                     IINTEARQVGKSPSFSINWNYGEPEAEIVNAITGRTDLDHISRISKCKLFYRFLRLLGK
                     IPTITNIHATNEPLMYNKCKEKATGFQAAKMALLKGFSKAKLGEWMKKPPEVDQFEWDE
                     DSLLKHIIYNSSYGSIS"
     CDS             1714..1815
                     /codon_start=1
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
                     /note="color: #cc99ff"
                     /translation="SGGSSGGSKRTADGSEFESPKKKRKVSGGSSGGS"
     CDS             join(1816..3795,3796..4052,4053..5912)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due
                     to the D10A mutation in the RuvC catalytic domain"
                     /note="This feature has 3 segments:
                        1: 1816 .. 3795 / #993366
                        2: 3796 .. 4052 / #993366
                        3: 4053 .. 5912 / #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLG"
     misc_feature    2473..2475
                     /label=R221K
                     /note="color: #ff0000"
     misc_feature    2992..2994
                     /label=N394K
                     /note="color: #ff0000"
     CDS             5933..5992
                     /codon_start=1
                     /label=2XSGGS XTEN 2XSGGS
                     /note="color: #993366"
                     /translation="GSSGSETPGTSESATPESS"
     CDS             6013..6594
                     /codon_start=1
                     /gene="SSB"
                     /gene_synonym="La; La/SSB; LARP3"
                     /product="lupus La protein"
                     /label=SSB
                     /note="La ribonucleoprotein domain family, member 3;
                     autoantigen La; SS-B/La protein; la autoantigen; sjoegren 
                     syndrome type B antigen; lupus La antigen; Sjogren syndrome
                     antigen B"
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS2237.1"
                     /db_xref="GeneID:6741"
                     /db_xref="HGNC:HGNC:11316"
                     /db_xref="MIM:109090"
                     /protein_id="NP_001281074.1"
                     /translation="MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEG
                     WVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEY
                     KNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIES
                     AKKFVETPGQKYKETDLLILFKDDYFAKKNE"
     CDS             6599..6632
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="GSKRTADGSE"
     CDS             6633..6660
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="/Original_Translation=KRTADGSEFEPKKKRKV"
                     /note="color: #993366"
                     /translation="ESPKKKRKV"
     CDS             6670..6696
                     /codon_start=1
                     /label=c-Myc NLS
                     /note="/created_by=chenp"
                     /note="color: #993366"
                     /translation="PAAKRVKLD"
     CDS             6705..6722
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #993366"
                     /translation="HHHHHH"
     polyA_signal    join(6751..6940,6941..6975)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="This feature has 2 segments:
                        1: 6751 .. 6940 / #a6acb3
                        2: 6941 .. 6975 / #a6acb3"
     misc_feature    6852
                     /label=mut
                     /note="color: #a6acb3"
     protein_bind    7070..7086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7094..7124)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    7139..7160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7448..8036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8207..9067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9068..9172)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     enhancer        join(9442..9690,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcccttc ttacagccca
      481 gtgaaaatga accccctggc ctgcctggac gccaagatgg aaagcgtgcc tcagatcttc
      541 gccgatcacg tggctacact gatcaacaag aagttcggcg agctgattaa gaacaaccct
      601 gcctacagcc ggcggagagt gctggccggc atcgtgatga gcgaggatta caatctggaa
      661 tcttgcaagg tcctgacagt gtccaccggc acaaaatgca tcagcggcga tagaatttct
      721 gccaacggct ccgtcctcaa cgactctcac gccgagatca tcagcagacg gtgcctgtgc
      781 aactacttct acagcgagct ggaaaagtac gtgaaggacg acaccggacg caacagcatc
      841 tttctgaagt ccccaaacaa gggcttcctg atcgacccca agtacaagtt ccacctgtac
      901 atcaccagcg ccccttgtgg cgactccaga ctgtttagcc cccacgacgt gtgtatcttc
      961 gacaagcacc ctaatagaaa ggcccggggc cagctgcgga ccaagatcga gagaggacag
     1021 ggcaccattc ctgttaatag ctccgatctg atccagacct gggatggcgt gctggtgggc
     1081 gagagactgc taacaatgtc ttgtagcgat aagctggcta agtggaatgt ggtgggtgtc
     1141 cagggcgctc tcctgagcaa cttcatccaa cctgtgtacc tggattctat catcctgggc
     1201 agcctgttcc accccagcca cctgtacagg gccgtgtacg gcagaatcga gaacgagatc
     1261 tacgacctga cccctccata cagactgaac aagcctaagc tgggcgccat catcaatacc
     1321 gaggccagac aggtgggcaa gagcccttca ttcagcatca actggaacta cggcgaacct
     1381 gaagccgaaa tcgtgaacgc catcacaggc agaaccgacc tggaccacat ctcccggatc
     1441 agcaagtgca agctgttcta ccggttcctg agactgctgg gaaaaatccc caccatcacc
     1501 aatatccacg ccaccaacga gcctctgatg tacaacaaat gcaaagagaa ggctacagga
     1561 tttcaggccg ctaaaatggc cctgctgaag ggcttttcta aggccaagct gggcgaatgg
     1621 atgaaaaagc cccccgaggt ggaccagttc gagtgggacg aggacagctt gctgaaacat
     1681 atcatttata acagcagcta tggaagcatc agctccggcg gaagctctgg tggcagcaag
     1741 cggaccgccg acggctctga attcgagagc cctaagaaga aaagaaaggt gagcggaggc
     1801 tctagcggcg gaagcgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg
     1861 ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc
     1921 aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc
     1981 gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag
     2041 aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc
     2101 ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac
     2161 cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac
     2221 cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg
     2281 gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc
     2341 gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc
     2401 gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg
     2461 agcaagagca gaaagctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc
     2521 ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc
     2581 gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac
     2641 aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg
     2701 tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc
     2761 ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa
     2821 gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag
     2881 aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc
     2941 aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaagagagag
     3001 gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg
     3061 ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac
     3121 cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc
     3181 aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg
     3241 aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc
     3301 aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag
     3361 tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag
     3421 cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac
     3481 cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac
     3541 tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat
     3601 ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg
     3661 gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg
     3721 aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac
     3781 accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc
     3841 aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg
     3901 atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag
     3961 ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc
     4021 atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc
     4081 gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac
     4141 agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg
     4201 aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg
     4261 cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac
     4321 gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg
     4381 ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg
     4441 aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag
     4501 ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc
     4561 atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac
     4621 tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc
     4681 accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc
     4741 gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc
     4801 ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac
     4861 gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac
     4921 ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag
     4981 atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag
     5041 ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa
     5101 aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc
     5161 gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc
     5221 cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa
     5281 ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag
     5341 aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc
     5401 aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct
     5461 gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg
     5521 tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag
     5581 ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc
     5641 tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag
     5701 caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc
     5761 aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac
     5821 accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac
     5881 gagacacgga tcgacctgtc tcagctggga ggtgactccg gaggatctag cggaggctcc
     5941 tctggctctg agacacctgg cacaagcgag agcgcaacac ctgaaagcag cgggggcagc
     6001 agcggggggt caatggctga aaatggtgat aatgaaaaga tggctgccct ggaggccaaa
     6061 atctgtcatc aaattgagta ttattttggc gacttcaatt tgccacggga caagtttcta
     6121 aaggaacaga taaaactgga tgaaggctgg gtacctttgg agataatgat aaaattcaac
     6181 aggttgaacc gtctaacaac agactttaat gtaattgtgg aagcattgag caaatccaag
     6241 gcagaactca tggaaatcag tgaagataaa actaaaatca gaaggtctcc aagcaaaccc
     6301 ctacctgaag tgactgatga gtataaaaat gatgtaaaaa acagatctgt ttatattaaa
     6361 ggcttcccaa ctgatgcaac tcttgatgac ataaaagaat ggttagaaga taaaggtcaa
     6421 gtactaaata ttcagatgag aagaacattg cataaagcat ttaagggatc aatttttgtt
     6481 gtgtttgata gcattgaatc tgctaagaaa tttgtagaga cccctggcca gaagtacaaa
     6541 gaaacagacc tgctaatact tttcaaggac gattactttg ccaaaaaaaa tgaatctggc
     6601 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagtctc ccaagaagaa gaggaaagtc
     6661 ggctctggcc ctgccgctaa gagagtgaag ctggactaac cggtcatcat caccatcacc
     6721 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6781 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6841 aataaaatga gaaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6901 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6961 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct
     7021 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7081 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat
     7141 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7201 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7261 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7321 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7381 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7441 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7501 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7561 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7621 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7681 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7741 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7801 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7861 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7921 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7981 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8041 atcctttgat cttttctacg gggtctgaca ctcagtggaa cgaaaactca cgttaaggga
     8101 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8161 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8221 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8281 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8341 taccgcgtga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8401 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8461 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8521 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8581 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8641 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8701 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8761 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8821 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8881 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8941 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9001 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9061 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9121 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9181 cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt
     9241 cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     9301 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     9361 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     9421 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     9481 cggggtcatt agttcatagc ccatatattg agttccgcgt tacataactt acggtaaatg
     9541 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     9601 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     9661 ctgcccactt ggcagtacat caagtgtatc
//
