LOCUS       snuABE_4.1dna           9660 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Molecular Genome Engineering Laboratory
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
     CDS             409..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             466..1683
                     /codon_start=1
                     /label=P. humanus ADARdd
                     /note="color: #ccffff"
                     /translation="LACLDAKMESVPQIFADHVATLINKKFGELIKNNPAYSRRRVLAG
                     IVMSEDYNLESCKVLTVSTGTKCISGDRISANGSVLNDSHAEIISRRCLCNYFYSELEK
                     YVKDDTGRNSIFLKSPNKGFLIDPKYKFHLYITSAPCGDSRLFSPHDVCIFDKHPNRKA
                     RGQLRTKIERGQGTIPVNSSDLIQTWDGVLVGERLLTMSCSDKLAKWNVVGVQGALLSN
                     FIQPVYLDSIILGSLFHPSHLYRAVYGRIENEIYDLTPPYRLNKPKLGAISNTEARQVG
                     KSPSFSINWNYGEPEAEIVNAITGRTDLDHISRISKCKLFYRFLRLLGKIPTITNIHAT
                     NEPLMYNKCKEKATGFQAAKMALLKGFSKAKLGFWMKKPPEVDQFEWDEDSLLKHIIYN
                     SSYGSIS"
     misc_feature    1282..1284
                     /label=I536S
                     /note="color: #a6acb3"
     misc_feature    1585..1587
                     /label=E637F
                     /note="color: #a6acb3"
     CDS             1684..1785
                     /codon_start=1
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
                     /note="color: #cc99ff"
                     /translation="SGGSSGGSKRTADGSEFESPKKKRKVSGGSSGGS"
     CDS             join(1786..3765,3766..4022,4023..5882)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due
                     to the D10A mutation in the RuvC catalytic domain"
                     /note="This feature has 3 segments:
                        1: 1786 .. 3765 / #993366
                        2: 3766 .. 4022 / #993366
                        3: 4023 .. 5882 / #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLG"
     misc_feature    2443..2445
                     /label=R221K
                     /note="color: #ff0000"
     misc_feature    2962..2964
                     /label=N394K
                     /note="color: #ff0000"
     CDS             5903..5962
                     /codon_start=1
                     /label=2XSGGS XTEN 2XSGGS
                     /note="color: #993366"
                     /translation="GSSGSETPGTSESATPESS"
     CDS             5983..6564
                     /codon_start=1
                     /gene="SSB"
                     /gene_synonym="La; La/SSB; LARP3"
                     /product="lupus La protein"
                     /label=SSB
                     /note="La ribonucleoprotein domain family, member 3;
                     autoantigen La; SS-B/La protein; la autoantigen; sjoegren 
                     syndrome type B antigen; lupus La antigen; Sjogren syndrome
                     antigen B"
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS2237.1"
                     /db_xref="GeneID:6741"
                     /db_xref="HGNC:HGNC:11316"
                     /db_xref="MIM:109090"
                     /protein_id="NP_001281074.1"
                     /translation="MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEG
                     WVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEY
                     KNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIES
                     AKKFVETPGQKYKETDLLILFKDDYFAKKNE"
     CDS             6569..6602
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="GSKRTADGSE"
     CDS             6603..6630
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="/Original_Translation=KRTADGSEFEPKKKRKV"
                     /note="color: #993366"
                     /translation="ESPKKKRKV"
     CDS             6640..6666
                     /codon_start=1
                     /label=c-Myc NLS
                     /note="/created_by=chenp"
                     /note="color: #993366"
                     /translation="PAAKRVKLD"
     CDS             6675..6692
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #993366"
                     /translation="HHHHHH"
     polyA_signal    join(6721..6910,6911..6945)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="This feature has 2 segments:
                        1: 6721 .. 6910 / #a6acb3
                        2: 6911 .. 6945 / #a6acb3"
     misc_feature    6822
                     /label=mut
                     /note="color: #a6acb3"
     protein_bind    7040..7056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7064..7094)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    7109..7130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7418..8006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8177..9037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9038..9142)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     enhancer        join(9412..9660,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcctggc ctgcctggac
      481 gccaagatgg aaagcgtgcc tcagatcttc gccgatcacg tggctacact gatcaacaag
      541 aagttcggcg agctgattaa gaacaaccct gcctacagcc ggcggagagt gctggccggc
      601 atcgtgatga gcgaggatta caatctggaa tcttgcaagg tcctgacagt gtccaccggc
      661 acaaaatgca tcagcggcga tagaatttct gccaacggct ccgtcctcaa cgactctcac
      721 gccgagatca tcagcagacg gtgcctgtgc aactacttct acagcgagct ggaaaagtac
      781 gtgaaggacg acaccggacg caacagcatc tttctgaagt ccccaaacaa gggcttcctg
      841 atcgacccca agtacaagtt ccacctgtac atcaccagcg ccccttgtgg cgactccaga
      901 ctgtttagcc cccacgacgt gtgtatcttc gacaagcacc ctaatagaaa ggcccggggc
      961 cagctgcgga ccaagatcga gagaggacag ggcaccattc ctgttaatag ctccgatctg
     1021 atccagacct gggatggcgt gctggtgggc gagagactgc taacaatgtc ttgtagcgat
     1081 aagctggcta agtggaatgt ggtgggtgtc cagggcgctc tcctgagcaa cttcatccaa
     1141 cctgtgtacc tggattctat catcctgggc agcctgttcc accccagcca cctgtacagg
     1201 gccgtgtacg gcagaatcga gaacgagatc tacgacctga cccctccata cagactgaac
     1261 aagcctaagc tgggcgccat cagcaatacc gaggccagac aggtgggcaa gagcccttca
     1321 ttcagcatca actggaacta cggcgaacct gaagccgaaa tcgtgaacgc catcacaggc
     1381 agaaccgacc tggaccacat ctcccggatc agcaagtgca agctgttcta ccggttcctg
     1441 agactgctgg gaaaaatccc caccatcacc aatatccacg ccaccaacga gcctctgatg
     1501 tacaacaaat gcaaagagaa ggctacagga tttcaggccg ctaaaatggc cctgctgaag
     1561 ggcttttcta aggccaagct gggcttctgg atgaaaaagc cccccgaggt ggaccagttc
     1621 gagtgggacg aggacagctt gctgaaacat atcatttata acagcagcta tggaagcatc
     1681 agctccggcg gaagctctgg tggcagcaag cggaccgccg acggctctga attcgagagc
     1741 cctaagaaga aaagaaaggt gagcggaggc tctagcggcg gaagcgacaa gaagtacagc
     1801 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1861 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1921 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     1981 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     2041 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     2101 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     2161 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     2221 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     2281 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     2341 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     2401 gacgccaagg ccatcctgtc tgccagactg agcaagagca gaaagctgga aaatctgatc
     2461 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     2521 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     2581 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     2641 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     2701 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     2761 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2821 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2881 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2941 gaggaactgc tcgtgaagct gaagagagag gacctgctgc ggaagcagcg gaccttcgac
     3001 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     3061 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     3121 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     3181 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     3241 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     3301 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     3361 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     3421 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     3481 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     3541 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     3601 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     3661 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3721 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3781 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3841 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3901 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3961 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     4021 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     4081 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     4141 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     4201 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     4261 gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc tcagagcttt
     4321 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     4381 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     4441 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     4501 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     4561 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     4621 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     4681 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4741 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     4801 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4861 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4921 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     4981 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     5041 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     5101 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     5161 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     5221 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     5281 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     5341 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     5401 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     5461 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     5521 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     5581 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     5641 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     5701 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     5761 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     5821 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5881 ggtgactccg gaggatctag cggaggctcc tctggctctg agacacctgg cacaagcgag
     5941 agcgcaacac ctgaaagcag cgggggcagc agcggggggt caatggctga aaatggtgat
     6001 aatgaaaaga tggctgccct ggaggccaaa atctgtcatc aaattgagta ttattttggc
     6061 gacttcaatt tgccacggga caagtttcta aaggaacaga taaaactgga tgaaggctgg
     6121 gtacctttgg agataatgat aaaattcaac aggttgaacc gtctaacaac agactttaat
     6181 gtaattgtgg aagcattgag caaatccaag gcagaactca tggaaatcag tgaagataaa
     6241 actaaaatca gaaggtctcc aagcaaaccc ctacctgaag tgactgatga gtataaaaat
     6301 gatgtaaaaa acagatctgt ttatattaaa ggcttcccaa ctgatgcaac tcttgatgac
     6361 ataaaagaat ggttagaaga taaaggtcaa gtactaaata ttcagatgag aagaacattg
     6421 cataaagcat ttaagggatc aatttttgtt gtgtttgata gcattgaatc tgctaagaaa
     6481 tttgtagaga cccctggcca gaagtacaaa gaaacagacc tgctaatact tttcaaggac
     6541 gattactttg ccaaaaaaaa tgaatctggc ggctcaaaaa gaaccgccga cggcagcgaa
     6601 ttcgagtctc ccaagaagaa gaggaaagtc ggctctggcc ctgccgctaa gagagtgaag
     6661 ctggactaac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
     6721 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6781 tggaaggtgc cactcccact gtcctttcct aataaaatga gaaaattgca tcgcattgtc
     6841 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6901 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     6961 gaaccagctg gggctcgata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7021 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7081 gcataaagtg taaagcctag ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7141 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7201 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgaca
     8041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8281 gcttaccatc tggccccagt gctgcaatga taccgcgtga cccacgctca ccggctccag
     8341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9181 cgggagatcg atctcccgat cccctagggt cgactctcag tacaatctgc tctgatgccg
     9241 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     9301 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     9361 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     9421 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatattg
     9481 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     9541 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     9601 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
//
