LOCUS       snuABE_3.1dna-SB        9990 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Molecular Genome Engineering Laboratory
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             409..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             466..1683
                     /codon_start=1
                     /label=P. humanus ADARdd
                     /translation="LACLDAKMESVPQIFADHVATLINKKFGELIKNNPAYSRRRVLAG
                     IVMSEDYNLESCKVLTVSTGTKCISGDRISANGSVLNDSHAEIISRRCLCNYFYSELEK
                     YVKDDTGRNSIFLKSPNKGFLIDPKYKFHLYITSAPCGDSRLFSPHDVCIFDKHPNRKA
                     RGQLRTKIERGQGTIPVNSSDLIQTWDGVLVGERLLTMSCSDKLAKWNVVGVQGALLSN
                     FIQPVYLDSIILGSLFHPSHLYRAVYGRIENEIYDLTPPYRLNKPKLGAISNTEARQVG
                     KSPSFSINWNYGEPEAEIVNAITGRTDLDHISRISKCKLFYRFLRLLGKIPTITNIHAT
                     NEPLMYNKCKEKATGFQAAKMALLKGFSKAKLGFWMKKPPEVDQFEWDEDSLLKHIIYN
                     SSYGSIS"
     misc_feature    1282..1284
                     /label=I536S
     misc_feature    1585..1587
                     /label=E637F
     CDS             1684..1785
                     /codon_start=1
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
                     /translation="SGGSSGGSKRTADGSEFESPKKKRKVSGGSSGGS"
     CDS             join(1786..3765,3766..4022,4023..5882)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLG"
     misc_feature    2443..2445
                     /label=R221K
     misc_feature    2962..2964
                     /label=N394K
     CDS             5903..5962
                     /codon_start=1
                     /label=2XSGGS XTEN 2XSGGS
                     /translation="GSSGSETPGTSESATPESS"
     CDS             join(5983..6124,6125..6126,6127..6564)
                     /codon_start=1
                     /gene="SSB"
                     /gene_synonym="La; La/SSB; LARP3"
                     /product="lupus La protein"
                     /label=SSB
                     /note="La ribonucleoprotein domain family, member 3;
                     autoantigen La; SS-B/La protein; la autoantigen; sjoegren 
                     syndrome type B antigen; lupus La antigen; Sjogren syndrome
                     antigen B"
                     /db_xref="CCDS:CCDS2237.1"
                     /db_xref="GeneID:6741"
                     /db_xref="HGNC:HGNC:11316"
                     /db_xref="MIM:109090"
                     /protein_id="NP_001281074.1"
                     /translation="MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEG
                     WVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEY
                     KNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIES
                     AKKFVETPGQKYKETDLLILFKDDYFAKKNE"
     CDS             6569..6602
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="GSKRTADGSE"
     CDS             6603..6630
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="/Original_Translation=KRTADGSEFEPKKKRKV"
                     /translation="ESPKKKRKV"
     CDS             6640..6662
                     /label=c-Myc NLS
                     /note="/created_by=chenp"
     misc_feature    6654..6662
                     /label=nonstandard type: USER
                     /note="/created_by=chenp"
     CDS             6667..6720
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6721..6966
                     /codon_start=1
                     /label=MLH1bp2(codon opt)
                     /translation="MSEEEEKRKIEEGMRRLRENLKKKRKEEEEKEKKETEELAKLAKE
                     IVEEAKKELKAEGKWKNKEEIWEAFKKKILEKFEKLE"
     CDS             6979..7005
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7015..7071
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7078..7323
                     /codon_start=1
                     /label=MLH1bp2
                     /translation="MSEEEEKRKIEEGMRRLRENLKKKRKEEEEKEKKETEELAKLAKE
                     IVEEAKKELKAEGKWKNKEEIWEAFKKKILEKFEKLE"
     CDS             7333..7350
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7379..7574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    7480
                     /label=mut
     rep_origin      complement(7748..8336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8507..9367)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9368..9472)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(9742..9990,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcctggc ctgcctggac
      481 gccaagatgg aaagcgtgcc tcagatcttc gccgatcacg tggctacact gatcaacaag
      541 aagttcggcg agctgattaa gaacaaccct gcctacagcc ggcggagagt gctggccggc
      601 atcgtgatga gcgaggatta caatctggaa tcttgcaagg tcctgacagt gtccaccggc
      661 acaaaatgca tcagcggcga tagaatttct gccaacggct ccgtcctcaa cgactctcac
      721 gccgagatca tcagcagacg gtgcctgtgc aactacttct acagcgagct ggaaaagtac
      781 gtgaaggacg acaccggacg caacagcatc tttctgaagt ccccaaacaa gggcttcctg
      841 atcgacccca agtacaagtt ccacctgtac atcaccagcg ccccttgtgg cgactccaga
      901 ctgtttagcc cccacgacgt gtgtatcttc gacaagcacc ctaatagaaa ggcccggggc
      961 cagctgcgga ccaagatcga gagaggacag ggcaccattc ctgttaatag ctccgatctg
     1021 atccagacct gggatggcgt gctggtgggc gagagactgc taacaatgtc ttgtagcgat
     1081 aagctggcta agtggaatgt ggtgggtgtc cagggcgctc tcctgagcaa cttcatccaa
     1141 cctgtgtacc tggattctat catcctgggc agcctgttcc accccagcca cctgtacagg
     1201 gccgtgtacg gcagaatcga gaacgagatc tacgacctga cccctccata cagactgaac
     1261 aagcctaagc tgggcgccat cagcaatacc gaggccagac aggtgggcaa gagcccttca
     1321 ttcagcatca actggaacta cggcgaacct gaagccgaaa tcgtgaacgc catcacaggc
     1381 agaaccgacc tggaccacat ctcccggatc agcaagtgca agctgttcta ccggttcctg
     1441 agactgctgg gaaaaatccc caccatcacc aatatccacg ccaccaacga gcctctgatg
     1501 tacaacaaat gcaaagagaa ggctacagga tttcaggccg ctaaaatggc cctgctgaag
     1561 ggcttttcta aggccaagct gggcttctgg atgaaaaagc cccccgaggt ggaccagttc
     1621 gagtgggacg aggacagctt gctgaaacat atcatttata acagcagcta tggaagcatc
     1681 agctccggcg gaagctctgg tggcagcaag cggaccgccg acggctctga attcgagagc
     1741 cctaagaaga aaagaaaggt gagcggaggc tctagcggcg gaagcgacaa gaagtacagc
     1801 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1861 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1921 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     1981 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     2041 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     2101 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     2161 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     2221 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     2281 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     2341 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     2401 gacgccaagg ccatcctgtc tgccagactg agcaagagca gaaagctgga aaatctgatc
     2461 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     2521 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     2581 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     2641 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     2701 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     2761 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2821 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2881 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2941 gaggaactgc tcgtgaagct gaagagagag gacctgctgc ggaagcagcg gaccttcgac
     3001 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     3061 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     3121 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     3181 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     3241 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     3301 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     3361 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     3421 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     3481 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     3541 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     3601 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     3661 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3721 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3781 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3841 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3901 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3961 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     4021 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     4081 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     4141 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     4201 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     4261 gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc tcagagcttt
     4321 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     4381 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     4441 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     4501 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     4561 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     4621 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     4681 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4741 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     4801 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4861 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4921 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     4981 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     5041 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     5101 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     5161 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     5221 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     5281 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     5341 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     5401 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     5461 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     5521 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     5581 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     5641 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     5701 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     5761 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     5821 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5881 ggtgactccg gaggatctag cggaggctcc tctggctctg agacacctgg cacaagcgag
     5941 agcgcaacac ctgaaagcag cgggggcagc agcggggggt caatggctga aaatggtgat
     6001 aatgaaaaga tggctgccct ggaggccaaa atctgtcatc aaattgagta ttattttggc
     6061 gacttcaatt tgccacggga caagtttcta aaggaacaga taaaactgga tgaaggctgg
     6121 gtacctctcg aaataatgat taagttcaat cggttgaacc ggctcacgac cgactttaat
     6181 gttatcgtgg aggccctgag taagagtaaa gccgaactta tggagatctc tgaggacaag
     6241 acaaagatta gaagatcacc tagtaaaccc ttgcctgagg tgactgatga atataagaac
     6301 gacgtcaaaa ataggtccgt ttatatcaag gggttcccaa cagacgccac ccttgacgat
     6361 attaaagagt ggctggagga taaaggtcag gtcctgaaca ttcagatgcg ccgcaccctt
     6421 cacaaggcct ttaaaggttc catattcgtc gtgttcgact ccatagaaag cgctaagaaa
     6481 tttgtggaaa ccccaggaca aaaatacaag gagactgatc tgcttatcct gtttaaggat
     6541 gactacttcg ccaaaaagaa tgagagcggc gggtcaaaga ggactgccga tggctctgag
     6601 tttgagtccc cgaaaaaaaa gcgcaaggtt ggtagcgggc cagcagcaaa gagggtgaaa
     6661 ctggacgagg gaagaggcag cctcctgaca tgcggagatg tggaagaaaa cccgggcccc
     6721 atgtccgagg aggaggaaaa gcggaagatc gaggagggta tgaggcgact gcgggaaaac
     6781 ctcaaaaaaa agcgaaaaga ggaggaggag aaagagaaga aagaaactga ggaattggcc
     6841 aagctggcaa aggaaatcgt agaagaggcg aagaaggaac tgaaggcgga aggcaaatgg
     6901 aaaaacaaag aagagatctg ggaagcattc aagaaaaaga tcctggagaa attcgaaaaa
     6961 ttggaatctg ggggatcacc cgcagctaag agagtgaaac tcgacggatc aggcgcgacg
     7021 aatttctctc tgctgaagca ggctggagat gtagaggaga accccggccc tggcaccatg
     7081 agcgaagagg aagagaagag aaagattgaa gaagggatgc gacgcctgag ggagaatttg
     7141 aagaagaagc ggaaagagga agaagaaaaa gagaaaaagg agacagaaga actcgctaag
     7201 ctcgccaaag aaattgtcga ggaggccaaa aaggagttga aagctgaggg gaagtggaag
     7261 aataaggagg agatttggga ggcttttaag aagaagatct tggaaaaatt tgagaaactt
     7321 gagtaaaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     7381 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7441 gaaggtgcca ctcccactgt cctttcctaa taaaatgaga aaattgcatc gcattgtctg
     7501 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     7561 gaagagaata gcagtcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8161 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8341 atcctttgat cttttctacg gggtctgaca ctcagtggaa cgaaaactca cgttaaggga
     8401 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8461 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8641 taccgcgtga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8821 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9121 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9481 cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt
     9541 cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     9601 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     9661 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     9721 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     9781 cggggtcatt agttcatagc ccatatattg agttccgcgt tacataactt acggtaaatg
     9841 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     9901 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     9961 ctgcccactt ggcagtacat caagtgtatc
//
