LOCUS       AAV_CMV-AcrIIA5_        5483 bp    DNA     circular SYN 06-AUG-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2025 from SnapGene 8.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(3368..5483,1..3367)
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     complement(55..74)
                     /label=T3 Primer
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=28
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    123..263
                     /label=AAV-4 ITR
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     enhancer        289..668
                     /note="CMV enhancer human cytomegalovirus immediate early 
                     enhancer"
     promoter        669..872
                     /note="CMV promoter human cytomegalovirus (CMV)
                     immediateearly promoter"
     promoter        917..935
                     /note="T7 promoter promoter for bacteriophage T7
                     RNApolymerase"
     CDS             955..1182
                     /codon_start=1
                     /label=AcrIIA5
                     /translation="MAYGKSRYNSYRKRNFSISDNQRREYAKKMKELEQAFENLDGWYL
                     SSMKDSAYKDFGKYEIRLSNHSADNRYHDLE"
     CDS             1192..1404
                     /codon_start=1
                     /label=AncGR2 C176
                     /label=AncGR2\C176
                     /note="/vntifkey=21
                     after Rihtar et al. 2023 
                     ('>https://www.nature.com/articles/s41589-022-01136-x)"
                     /translation="NSSQNWQRFYQLTKLLDSMHEMVGGLLQFCFYTFVNKSLSVEFPE
                     MLAEIISNQLPKFNAGSVKPLLFHQK"
     CDS             1408..1452
                     /codon_start=1
                     /label=(GGS)5 Linker
                     /translation="GGSGGSGGSGGSGGS"
     CDS             1456..1980
                     /codon_start=1
                     /label=AncGR2N175
                     /note="/vntifkey=21
                     after Rihtar et al. 2023 
                     ('>https://www.nature.com/articles/s41589-022-01136-x)"
                     /translation="LISLLEVIEPEVLYSGYDSTLPDTSTRLMSTLNRLGGRQVVSAVK
                     WAKALPGFRNLHLDDQMTLLQYSWMSLMAFSLGWRSYKQSNGNMLCFAPDLVINEERMQ
                     LPYMYDQCQQMLKISSEFVRLQVSYDEYLCMKVLLLLSTVPKDGLKSQAVFDEIRMTYI
                     KELGKAIVKREG"
     CDS             1990..2184
                     /codon_start=1
                     /label=AcrIIA5
                     /translation="NGRLIVNVKASKLNFVDIIENKLGKIIEKIDTLDLDKYRFINATK
                     LERDIKCYYKGYKTKKDVI"
     polyA_signal    2291..2515
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    complement(2538..2678)
                     /label=AAV-2 ITR
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     promoter        3391..3495
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3496..3564,3565..4356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
       61 ccctcactaa agggaacaaa agctgggtac cactagtatg catcctgcag gacgcgtcct
      121 acccactccc tctatgcgcg ctcgctcact cactcggccc tggagaccaa aggtctccag
      181 actgccggcc tctggccggc agggccgagt gagtgagcga gcgcgcatag agggagtggg
      241 tacctccatc atctaggttt gccagatctc gggccagata tacgcgttga cattgattat
      301 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      361 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      481 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      541 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      601 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      661 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      721 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      781 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      841 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
      901 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagcatggca
      961 tacggtaaaa gccgatataa ctcttaccgg aaacgaaatt ttagtatcag tgacaaccag
     1021 agaagagaat acgccaaaaa aatgaaggaa ttggagcaag cgtttgagaa tttggacggg
     1081 tggtacctct catccatgaa ggatagcgcc tacaaagact tcggaaagta cgagataaga
     1141 ctgtcaaacc acagtgcaga caataggtat cacgaccttg aaggtccggg gaacagcagc
     1201 cagaactggc agaggttcta ccagctgacc aagctgctgg acagcatgca cgagatggtg
     1261 ggcggcctgc tccagttctg cttctacacc ttcgtgaaca agagcctgag cgtggagttc
     1321 cccgagatgc tggccgagat catcagcaac cagctgccta agtttaatgc cggcagcgtg
     1381 aagcccctgc tgttccacca gaaggggggc ggaagcggcg gatccggcgg atctggcgga
     1441 tcgggcggga gtggactgat cagcctgctg gaggtgatcg agcccgaggt gctgtacagc
     1501 ggctacgaca gcaccctgcc cgacaccagc accaggctga tgagcaccct gaacaggctg
     1561 ggcggcaggc aggtggtgag cgccgtgaag tgggccaagg ccctgcccgg cttcaggaac
     1621 ctgcacctgg acgaccagat gaccctgctg cagtacagct ggatgagcct gatggccttc
     1681 agcctgggct ggaggagcta caagcagagc aacggcaaca tgctgtgctt cgcccccgac
     1741 ctggtgatca acgaggagag gatgcagctg ccctacatgt acgaccagtg ccagcagatg
     1801 ctgaagatca gcagcgagtt cgtgaggctg caggtgagct acgacgagta cctgtgcatg
     1861 aaggtgctgc tgctgctgag caccgtgccc aaggacggcc tgaagagcca ggccgtgttc
     1921 gacgagatca ggatgaccta catcaaggag ctgggcaagg ccatcgtgaa gagggagggc
     1981 ggtccgggga atggtcgcct tatcgttaat gtaaaggctt caaaacttaa tttcgttgat
     2041 attatagaga acaagctggg caaaattatt gagaagatcg atacgcttga tttggataag
     2101 tacagattca ttaacgctac taaactggaa agggatataa aatgttacta taaggggtat
     2161 aaaacaaaaa aggatgttat ctgactcgag cggccgccac tgtgctggat atctgcagaa
     2221 ttccaccaca ctggactagt ggatccgagc tcggtaccaa gcttaagttt aaaccgctga
     2281 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     2341 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     2401 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     2461 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     2521 gaggcggaaa gagatctagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     2581 tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc
     2641 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc cccccccccc cccccccctg
     2701 cattctagag agctccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     2761 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     2821 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     2881 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     2941 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3001 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3061 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3121 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3181 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3241 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3301 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     3361 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3421 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3481 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3541 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3601 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3661 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3721 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3781 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3841 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3901 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3961 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4021 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4081 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4141 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4201 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4261 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4321 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4381 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4441 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4501 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4561 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4621 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     4681 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4741 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4801 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4861 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4921 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4981 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5041 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5101 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5161 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5221 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5281 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5341 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5401 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     5461 cgtatgttgt gtggaattgt gag
//
