LOCUS       2286_AAV_H1_GRIN        7338 bp DNA     circular SYN 04-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2024 from SnapGene Viewer 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7338
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    1..130
                     /label=L-ITR
     primer_bind     133..170
                     /label=Primer 2
     misc_feature    137..142
                     /label=XbaI
                     /note="XbaI
                     Geneious type: restriction"
     misc_feature    146..242
                     /label=H1 promoter with additional G
     misc_RNA        269..344
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_feature    272
                     /label=G/C/A?
     misc_feature    299
                     /label=?C/G/T
     CDS             311..364
                     /codon_start=1
                     /label=Feature 54
                     /translation="MPCLSRQLVGEIFSTGAT"
     primer_bind     complement(318..362)
                     /label=Primer 5
     primer_bind     complement(324..349)
                     /label=SaCas_Scaffold_ClaI_re
     misc_feature    359..368
                     /label=Kozak
     primer_bind     364..388
                     /label=#609_SaCas9_BbsI_v2_fw
     misc_feature    365..368
                     /label=NLS
                     /note="NLS"
     misc_feature    369..415
                     /label=NLS
                     /note="NLS"
     primer_bind     392..418
                     /label=SaCas9_BbsI_fw
     CDS             416..3571
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Staphylococcus 
                     aureus Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
                     RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
                     SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
                     RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
                     KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
                     IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
                     IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
                     NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
                     VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
                     YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
                     EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
                     VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
                     KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
                     ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
                     DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
                     NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
                     DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
                     RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKG"
     misc_feature    815..817
                     /label=Ub high
                     /note="Ub high"
     primer_bind     909..931
                     /label=hSaCas9_Seq1_fw
     primer_bind     complement(1694..1714)
                     /label=MH_SaN_Split_434_BbsI_BsmBI_re
     primer_bind     complement(1696..1718)
                     /label=MH_SaN_Split_435_BbsI_BsmBI_re
     primer_bind     complement(1697..1720)
                     /label=MH_SaN_Split_436_BbsI_BsmBI_re
     primer_bind     complement(1702..1724)
                     /label=MH_SaN_Split_437_BbsI_BsmBI_re
     primer_bind     complement(1705..1727)
                     /label=MH_SaN_Split_438_BbsI_BsmBI_re
     primer_bind     1715..1733
                     /label=MH_SaC_Split_435_BbsI_BsmBI_fw
     primer_bind     1718..1735
                     /label=MH_SaC_Split_436_BbsI_BsmBI_fw
     primer_bind     1721..1738
                     /label=MH_SaC_Split_437_BbsI_BsmBI_fw
     primer_bind     1724..1741
                     /label=MH_SaC_Split_438_BbsI_BsmBI_fw
     primer_bind     1725..1744
                     /label=hSaCas9_Seq2_fw
     primer_bind     1727..1747
                     /label=MH_SaC_Split_439_BbsI_BsmBI_fw
     primer_bind     2538..2561
                     /label=hSaCas9_Seq3_fw
     primer_bind     complement(2569..2594)
                     /label=SaN_split_727_BbsI_BsmBI_re
     primer_bind     complement(2591..2614)
                     /label=DN_SaN_split734_BbsI_BsmBI_re
     primer_bind     2594..2620
                     /label=SaC_split_728_BbsI_BsmBI_fw
     CDS             2600..2647
                     /codon_start=1
                     /label=split loop 2
                     /translation="NQMFEEKQAESMPEIE"
     primer_bind     complement(2608..2631)
                     /label=SaCasN_split739_BbsI_BsmBI_re
     primer_bind     complement(2608..2631)
                     /label=DN_SaN_split739_BbsI_BsmBI_re
     primer_bind     complement(2608..2629)
                     /label=SaCas9_739_BbsI_re
     CDS             2612..2617
                     /codon_start=1
                     /label=Aplit 734/735
                     /translation="EE"
     primer_bind     complement(2615..2642)
                     /label=DN_SaN_split743_BbsI_BsmBI_re
     primer_bind     2615..2636
                     /label=DN_SaC_split735_BbsI_BsmBI_fw
     misc_feature    2618..2620
                     /label=Ub high - AAG = K (original), AGG = R
                     /note="Ub high - AAG = K (original), AGG = R"
     primer_bind     complement(2627..2648)
                     /label=SaN_split_745_BbsI_BsmBI_re
     CDS             2627..2632
                     /codon_start=1
                     /label=split 739/740
                     /translation="ES"
     primer_bind     2630..2650
                     /label=DN_SaC_split740_BbsI_BsmBI_fw
     CDS             2639..2644
                     /codon_start=1
                     /label=Split 743/744
                     /translation="EI"
     primer_bind     2642..2666
                     /label=DN_SaC_split744_BbsI_BsmBI_fw
     primer_bind     2648..2671
                     /label=SaC_split_746_BbsI_BsmBI_fw
     primer_bind     3355..3374
                     /label=hSaCas9_Seq4_fw
     primer_bind     complement(3547..3573)
                     /label=SaCas9_BbsI_re
     misc_feature    3572..3619
                     /label=NLS
                     /note="NLS"
     misc_feature    3620
                     /label=BamHI
                     /note="BamHI"
     primer_bind     3676..3702
                     /label=Primer 2
     CDS             3682..4530
                     /codon_start=1
                     /label=AcrIIA5
                     /translation="AYGKSRYNSYRKRNFSISDNQRREYAKKMKELEQAFENLDGWYLS
                     SMKDSAYKDFGKYEIRLSNHSADNRYHDLELATTLERIEKNFVITDPRLPDNPIIFASD
                     SFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFW
                     NLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKELNGRL
                     IVNVKASKLNFVDIIENKLGKIIEKIDTLDLDKYRFINATKLERDIKCYYKGYKTKKDV
                     I"
     misc_difference 3682..3691
                     /label=reg1
     primer_bind     3683..3712
                     /label=2521_SV40-AIIA5_fw
     misc_feature    3692..3718
                     /label=Helix
     misc_feature    3719..3736
                     /label=reg1
     misc_feature    3737..3796
                     /label=Helix
     primer_bind     complement(3773..3800)
                     /label=2514_I12linker_rv
     misc_feature    3797..3805
                     /label=reg2
     primer_bind     3800..3820
                     /label=2516_I12linker_fw
     misc_feature    3806..3820
                     /label=Helix
     misc_feature    3821..3826
                     /label=reg3
     misc_feature    3827..3844
                     /label=Feature 23
     misc_feature    3845..3853
                     /label=reg4
     misc_feature    3854..3868
                     /label=Sheet
     misc_feature    3869..3877
                     /label=Feature 33
     primer_bind     complement(3883..3906)
                     /label=2513_I32linker_rv
     misc_feature    3887..3895
                     /label=Feature 34
     misc_feature    3902..4342
                     /label=Feature 35
     primer_bind     3907..3926
                     /label=2512_GPG_LOV_fw
     primer_bind     3907..3926
                     /label=2511_GSG_LOV_fw
     CDS             3908..4336
                     /codon_start=1
                     /label=AsLOV2 hs
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     primer_bind     3908..3926
                     /label=2509_G_LOV_fw
     primer_bind     3908..3926
                     /label=2510_GS_LOV_fw
     primer_bind     complement(4223..4252)
                     /label=2507 RV RLRD/RVHD_LOV2
     primer_bind     4259..4277
                     /label=2506 FW RLRD_LOV2
     primer_bind     4262..4277
                     /label=2508 FW RVHD_LOV2
     primer_bind     complement(4318..4335)
                     /label=2517_G_LOV_rv
     primer_bind     complement(4318..4335)
                     /label=2518_SG_LOV_rv
     primer_bind     complement(4318..4335)
                     /label=2520_GPG_LOV_rv
     primer_bind     complement(4318..4335)
                     /label=2519_GSG_LOV_rv
     primer_bind     4337..4358
                     /label=2515_I32linker_fw
     misc_feature    4343..4363
                     
     misc_feature    4364..4369
                     /label=Helix
     misc_feature    4367..4378
                     /label=reg6
     misc_feature    4379..4423
                     /label=Helix
     misc_feature    4424..4438
                     /label=reg7
     misc_feature    4439..4459
                     /label=Helix
     misc_feature    4460..4462
                     /label=reg8
     misc_feature    4463..4477
                     /label=Helix
     misc_feature    4478..4486
                     /label=reg9
     misc_feature    4487..4498
                     /label=Sheet
     primer_bind     complement(4495..4528)
                     /label=2543_AIIA5-SV40_rv
     primer_bind     complement(4510..4534)
                     /label=Primer 1
     misc_feature    4538..4597
                     /label=polyA
     misc_feature    4601..4741
                     /label=R-ITR
     rep_origin      4833..5139
                     /label=f1 Origin
     misc_feature    5658..6515
                     /label=AmpR
     rep_origin      6666..7333
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gactccatat ttgcatgtcg ctatgtgttc tgggaaatca
      181 ccataaacgt gaaatgtctt tggatttggg aatcttataa gttctgtatg aggaccacgg
      241 tacaccgaga gtaggctggt agatggaggt tttagtactc tggaaacaga atctactaaa
      301 acaaggcaaa atgccgtgtt tatctcgtca acttgttggc gagatttttt ccaccggtgc
      361 caccatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccaagcg
      421 gaactacatc ctgggcctgg acatcggcat caccagcgtg ggctacggca tcatcgacta
      481 cgagacacgg gacgtgatcg atgccggcgt gcggctgttc aaagaggcca acgtggaaaa
      541 caacgagggc aggcggagca agagaggcgc cagaaggctg aagcggcgga ggcggcatag
      601 aatccagaga gtgaagaagc tgctgttcga ctacaacctg ctgaccgacc acagcgagct
      661 gagcggcatc aacccctacg aggccagagt gaagggcctg agccagaagc tgagcgagga
      721 agagttctct gccgccctgc tgcacctggc caagagaaga ggcgtgcaca acgtgaacga
      781 ggtggaagag gacaccggca acgagctgtc caccaaagag cagatcagcc ggaacagcaa
      841 ggccctggaa gagaaatacg tggccgaact gcagctggaa cggctgaaga aagacggcga
      901 agtgcggggc agcatcaaca gattcaagac cagcgactac gtgaaagaag ccaaacagct
      961 gctgaaggtg cagaaggcct accaccagct ggaccagagc ttcatcgaca cctacatcga
     1021 cctgctggaa acccggcgga cctactatga gggacctggc gagggcagcc ccttcggctg
     1081 gaaggacatc aaagaatggt acgagatgct gatgggccac tgcacctact tccccgagga
     1141 actgcggagc gtgaagtacg cctacaacgc cgacctgtac aacgccctga acgacctgaa
     1201 caatctcgtg atcaccaggg acgagaacga gaagctggaa tattacgaga agttccagat
     1261 catcgagaac gtgttcaagc agaagaagaa gcccaccctg aagcagatcg ccaaagaaat
     1321 cctcgtgaac gaagaggata ttaagggcta cagagtgacc agcaccggca agcccgagtt
     1381 caccaacctg aaggtgtacc acgacatcaa ggacattacc gcccggaaag agattattga
     1441 gaacgccgag ctgctggatc agattgccaa gatcctgacc atctaccaga gcagcgagga
     1501 catccaggaa gaactgacca atctgaactc cgagctgacc caggaagaga tcgagcagat
     1561 ctctaatctg aagggctata ccggcaccca caacctgagc ctgaaggcca tcaacctgat
     1621 cctggacgag ctgtggcaca ccaacgacaa ccagatcgct atcttcaacc ggctgaagct
     1681 ggtgcccaag aaggtggacc tgtcccagca gaaagagatc cccaccaccc tggtggacga
     1741 cttcatcctg agccccgtcg tgaagagaag cttcatccag agcatcaaag tgatcaacgc
     1801 catcatcaag aagtacggcc tgcccaacga catcattatc gagctggccc gcgagaagaa
     1861 ctccaaggac gcccagaaaa tgatcaacga gatgcagaag cggaaccggc agaccaacga
     1921 gcggatcgag gaaatcatcc ggaccaccgg caaagagaac gccaagtacc tgatcgagaa
     1981 gatcaagctg cacgacatgc aggaaggcaa gtgcctgtac agcctggaag ccatccctct
     2041 ggaagatctg ctgaacaacc ccttcaacta tgaggtggac cacatcatcc ccagaagcgt
     2101 gtccttcgac aacagcttca acaacaaggt gctcgtgaag caggaagaaa acagcaagaa
     2161 gggcaaccgg accccattcc agtacctgag cagcagcgac agcaagatca gctacgaaac
     2221 cttcaagaag cacatcctga atctggccaa gggcaagggc agaatcagca agaccaagaa
     2281 agagtatctg ctggaagaac gggacatcaa caggttctcc gtgcagaaag acttcatcaa
     2341 ccggaacctg gtggatacca gatacgccac cagaggcctg atgaacctgc tgcggagcta
     2401 cttcagagtg aacaacctgg acgtgaaagt gaagtccatc aatggcggct tcaccagctt
     2461 tctgcggcgg aagtggaagt ttaagaaaga gcggaacaag gggtacaagc accacgccga
     2521 ggacgccctg atcattgcca acgccgattt catcttcaaa gagtggaaga aactggacaa
     2581 ggccaaaaaa gtgatggaaa accagatgtt cgaggaaaag caggccgaga gcatgcccga
     2641 gatcgaaacc gagcaggagt acaaagagat cttcatcacc ccccaccaga tcaagcacat
     2701 taaggacttc aaggactaca agtacagcca ccgggtggac aagaagccta atagagagct
     2761 gattaacgac accctgtact ccacccggaa ggacgacaag ggcaacaccc tgatcgtgaa
     2821 caatctgaac ggcctgtacg acaaggacaa tgacaagctg aaaaagctga tcaacaagag
     2881 ccccgaaaag ctgctgatgt accaccacga cccccagacc taccagaaac tgaagctgat
     2941 tatggaacag tacggcgacg agaagaatcc cctgtacaag tactacgagg aaaccgggaa
     3001 ctacctgacc aagtactcca aaaaggacaa cggccccgtg atcaagaaga ttaagtatta
     3061 cggcaacaaa ctgaacgccc atctggacat caccgacgac taccccaaca gcagaaacaa
     3121 ggtcgtgaag ctgtccctga agccctacag attcgacgtg tacctggaca atggcgtgta
     3181 caagttcgtg accgtgaaga atctggatgt gatcaaaaaa gaaaactact acgaagtgaa
     3241 tagcaagtgc tatgaggaag ctaagaagct gaagaagatc agcaaccagg ccgagtttat
     3301 cgcctccttc tacaacaacg atctgatcaa gatcaacggc gagctgtata gagtgatcgg
     3361 cgtgaacaac gacctgctga accggatcga agtgaacatg atcgacatca cctaccgcga
     3421 gtacctggaa aacatgaacg acaagaggcc ccccaggatc attaagacaa tcgcctccaa
     3481 gacccagagc attaagaagt acagcacaga cattctgggc aacctgtatg aagtgaaatc
     3541 taagaagcac cctcagatca tcaaaaaggg caaaaggccg gcggccacga aaaaggccgg
     3601 ccaggcaaaa aagaaaaagg gatccgctac aaactttagc cttctcaaac aagcaggaga
     3661 tgtcgaagag aatcctggcc cggcatacgg taaaagccga tataactctt accggaaacg
     3721 aaattttagt atcagtgaca accagagaag agaatacgcc aaaaaaatga aggaattgga
     3781 gcaagcgttt gagaatttgg acgggtggta cctctcatcc atgaaggata gcgcctacaa
     3841 agacttcgga aagtacgaga taagactgtc aaaccacagt gcagacaata ggtatcacga
     3901 ccttgaactt gctacaactt tggagcggat tgagaagaat ttcgtcataa ctgatcccag
     3961 gttgcctgat aatcctataa tattcgcctc cgatagtttt cttcaactta cggagtactc
     4021 cagagaggag atactgggaa ggaattgcag attcctccaa ggtccagaaa ctgatagagc
     4081 tactgtgagg aagatacggg atgccattga caatcaaact gaagtgacag tgcaacttat
     4141 caattacacc aagtcaggga aaaaattctg gaacctgttc catttgcaac ccatgaggga
     4201 ccaaaagggc gatgtgcaat atttcatagg cgttcaactt gatggtactg aacacgtcag
     4261 agatgcggcg gagcgagaag gagtgatgct gattaagaaa acagcagaga atatcgacga
     4321 agcggcgaaa gagctgaatg gtcgccttat cgttaatgta aaggcttcaa aacttaattt
     4381 cgttgatatt atagagaaca agctgggcaa aattattgag aagatcgata cgcttgattt
     4441 ggataagtac agattcatta acgctactaa actggaaagg gatataaaat gttactataa
     4501 ggggtataaa acaaaaaagg atgttatctg agaattctag caataaagga tcgtttattt
     4561 tcattggaag cgtgtgttgg ttttttgatc aggcgcgggc aggaacccct agtgatggag
     4621 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     4681 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     4741 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     4801 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4861 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4921 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4981 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     5041 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5101 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     5161 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5221 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     5281 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5341 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5401 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5461 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5521 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5581 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5641 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5701 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5761 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5821 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5881 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5941 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6001 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6061 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6121 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6181 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6241 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6301 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6361 ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6421 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6481 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6541 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7081 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7141 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7201 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7261 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7321 ggccttttgc tcacatgt
//
