LOCUS       Exported                4882 bp ds-DNA     circular UNA 28-MAR-2023
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Escherichia virus T4
  ORGANISM  Escherichia virus T4
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 19, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SnapGene NotesSequence Type: SyntheticConfirmed Experimentally: 
            0Transformed Into: EC100D(TM) pir+SnapGene UUID: 
            2a4f7f73-b25f-48ff-b186-80b2df678a09Sequence Document Type: UNAOther
            Notes: CreatedBy: Brady CressImported FromPath: 
            /Users/benjaminadler/Library/CloudStorage/GoogleDrive-ba14@berkeley.
            edu/My Drive/BA_Doudna_Lab/BA_DNA_DB/BA_SnapGene/DNA 
            Files/Phage-based anti-defenseFilename: pBA1398_pJEX-T4acb1_OE.dna
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Escherichia virus T4"
                     /mol_type="genomic DNA"
     source          115..573
                     /organism="Escherichia virus T4"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:10665"
     misc_feature    1..79
                     /label=pJEX-D Promoter
     misc_feature    56..79
                     /label=EilO operator
     primer_bind     complement(72..114)
                     /label=oBA2269_pJEX-SC101Gib_R
     misc_feature    102..107
                     /label=RBS
     primer_bind     112..145
                     /label=oBA2435_T4acb1_OE_F
     misc_feature    113..114
                     /label=2>3
     gene            115..573
                     /gene="57B"
                     /locus_tag="T4p137"
                     /label=57B
                     /db_xref="GeneID:1258596"
     CDS             115..573
                     /codon_start=1
                     /transl_table=8
                     /gene="57B"
                     /locus_tag="T4p137"
                     /product="hypothetical protein"
                     /label=57B
                     /db_xref="GeneID:1258596"
                     /protein_id="NP_049750.1"
                     /translation="MMEFKDFSTGLYVAAKFSELTLDALEELQRSLRVPNPVPREKIHS
                     TICYSRVNVPYVPSSGSFEVASSGHLEVWKTQDGSTLVLVLDSEYLRCRHMYARALGAT
                     HDFDDYTPHITLSYNVGPLSFSGDVQIPVVLDREYKEPLKLDWADDLK"
     CDS             115..573
                     /codon_start=1
                     /transl_table=0
                     /label=acb1
                     /translation="MMEFKDFSTGLYVAAKFSELTLDALEELQRSLRVPNPVPREKIHS
                     TICYSRVNVPYVPSSGSFEVASSGHLEVWKTQDGSTLVLVLDSEYLRCRHMYARALGAT
                     HDFDDYTPHITLSYNVGPLSFSGDVQIPVVLDREYKEPLKLDWADDLK"
     gene            115..118
                     /gene="57A"
                     /locus_tag="T4p138"
                     /label=57A
                     /db_xref="GeneID:1258624"
     CDS             115..118
                     /codon_start=1
                     /transl_table=8
                     /gene="57A"
                     /locus_tag="T4p138"
                     /product="gp57A chaperone for tail fiber formation"
                     /label=57A
                     /note="molecular chaperone for both short and long tail
                     fibers"
                     /db_xref="GeneID:1258624"
                     /protein_id="NP_049751.1"
                     /translation=""
     primer_bind     complement(539..578)
                     /label=oBA2436_T4acb1_OE_R
     primer_bind     574..604
                     /label=oBA2261_express_common_F
     misc_feature    574..577
                     /label=4bp BsaI
     terminator      581..709
                     /label=BBa_B0015 Terminator
     primer_bind     581..612
                     /label=oBFC0174
     primer_bind     581..604
                     /label=oBFC1711
     primer_bind     589..610
                     /label=oBFC0177
     primer_bind     complement(617..660)
                     /label=oBFC0177
     primer_bind     complement(641..667)
                     /label=oBFC1189
     primer_bind     complement(683..709)
                     /label=oBFC0696
     primer_bind     complement(686..710)
                     /label=oBFC0055
     primer_bind     complement(686..710)
                     /label=oBFC0806
     primer_bind     complement(686..709)
                     /label=oBFC0422
     primer_bind     complement(686..709)
                     /label=oBFC0426
     primer_bind     complement(686..709)
                     /label=oBFC0488
     primer_bind     complement(686..709)
                     /label=oBFC0490
     primer_bind     complement(687..730)
                     /label=oBA1653_RFP924_droupout_Gib_R
     primer_bind     complement(688..736)
                     /label=oBA1708_RFPdropout_part4_gib_R
     primer_bind     complement(688..724)
                     /label=oBFC1287
     primer_bind     complement(688..710)
                     /label=oBFC0309
     primer_bind     complement(688..710)
                     /label=oBFC1335
     primer_bind     complement(688..710)
                     /label=oBFC0785
     primer_bind     complement(688..710)
                     /label=oBFC0356
     primer_bind     complement(688..709)
                     /label=oBFC0692
     primer_bind     complement(688..703)
                     /label=oBFC1196
     primer_bind     690..733
                     /label=oBA1650_pBFC0924_Gib_F
     primer_bind     complement(692..710)
                     /label=oBFC1204
     primer_bind     complement(692..710)
                     /label=oBFC0738
     primer_bind     complement(702..730)
                     /label=oBFC1476
     primer_bind     710..759
                     /label=oBA1710_SC101_part4_gib_R
     misc_feature    710..736
                     /label=Pt4_common_region_6>4
     primer_bind     complement(715..737)
                     /label=oBFC1462
     misc_feature    718..721
                     /label=6>4 OH
     misc_feature    complement(722..725)
                     /label=SMRT Gate OH Right
     misc_feature    complement(730..736)
                     /label=AarI
     primer_bind     735..761
                     /label=oBFC0802
     misc_feature    737..2999
                     /label=SC101_part
     primer_bind     737..761
                     /label=oBFC0693
     rep_origin      complement(776..2244)
                     /direction=LEFT
                     /label=SC101
                     /note="/snapgene_direction=LEFT"
     primer_bind     complement(937..960)
                     /label=oBFC0727
     CDS             complement(1294..2244)
                     /codon_start=1
                     /transl_table=0
                     /gene="<i>rep101</i>"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2292..2514
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     complement(2974..2996)
                     /label=oBFC0813a
     primer_bind     complement(2974..2996)
                     /label=oBFC0813
     primer_bind     complement(2980..3029)
                     /label=oBA1709_SC101_part4_gib_F
     misc_feature    2996..2998
                     /label=BsmBI_OH
     primer_bind     3000..3059
                     /label=oBA1718_kanR_part4_gib_R
     misc_feature    3000..3029
                     /label=Part4_Ori_AbR_Common
     terminator      complement(3030..3056)
                     /label=KanR Terminator
     primer_bind     3030..3055
                     /label=oBFC0313
     CDS             complement(3057..3872)
                     /label=KanR
     primer_bind     complement(3141..3168)
                     /label=oBFC0064
     promoter        complement(3873..4020)
                     /label=KanR Promoter
     primer_bind     4021..4074
                     /label=oBA2268_pJEX-SC101Gib_F
     misc_feature    4021..4045
                     /label=Pt4_common_region_4>5
     primer_bind     complement(4021..4045)
                     /label=oBA2260_pt4_common_4>5_R
     misc_feature    4021..4027
                     /label=AarI
     primer_bind     complement(4027..4045)
                     /label=oBA1651_pBFC0924_Gib_R
     misc_feature    4032..4035
                     /label=SMRT Gate OH Left
     misc_feature    4036..4037
                     /label=spacer in case extra trimming during AarI GG
     misc_feature    4038..4041
                     /label=pBBR1 Backbone Index (GACT)
     misc_feature    4042..4045
                     /label=4>5 OH
                     /label=4b(3)
     misc_feature    4042..4045
                     /label=BsaI OH
     misc_feature    4046..4996
                     /label=from gbBA189
     misc_feature    4046..4049
                     /label=1>2
     CDS             complement(4056..4634)
                     /codon_start=1
                     /transl_table=0
                     /label=eilR
                     /translation="MGYLNREERRETIMQAAMRVALDQGFTGMTVRNIATAAGVAAGQV
                     HHHFTSSGELKSQAFIRVIREMMDLQRLSRTAGWREQLFSALGSEDGRLEPYIRLWRQA
                     QLLADSDPEIKSAYLLTMNLWHDEAVRIIRAGHAAGEFTLRDSAENIAWRLISLVCGLD
                     GIYVLGMPEVDDAAFTRHLQHVIQLELFS"
     misc_feature    4092
                     /label=AarI removal
     misc_feature    4635..4649
                     /label=GSG_RBS
     RBS             4641..4649
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in <i>E. coli</i>; complementary 
                     to a region in the 3' end of the 16S rRNA (<a 
                     href=""https://www.ncbi.nlm.nih.gov/pubmed/7528374"" 
                     title=""https://www.ncbi.nlm.nih.gov/pubmed/7528374"">Chen 
                     et al., 1994</a>)"
     misc_feature    complement(4657..4685)
                     /label=pFAB weak constitutive
ORIGIN
        1 aaaaaactgc aaaaaatagt ttgacaggac acgtgtccaa ctttaagatg tacccaaagt
       61 tggacacgtg tccaactttg aattcaaaag atcttttaag aaggagatat actgatgatg
      121 gaatttaaag acttttcaac gggtctttat gtagcagcta agttttcaga attaacactt
      181 gatgcgctgg aagaacttca gcgctcttta cgtgttccta atccagttcc tagagaaaaa
      241 attcattcga ctatatgtta ttcaagagta aatgttccat atgttccatc gagtggaagt
      301 tttgaagtag cttcttctgg acatttagaa gtatggaaaa cacaagatgg atcgactctt
      361 gtacttgtgc tagattctga atatctgcgc tgtcgacaca tgtatgcgcg ggcactaggt
      421 gctacacatg attttgatga ttacacaccg catataacat tgtcttataa tgttgggccc
      481 ttatcattta gcggtgatgt acaaattccg gtcgtattag atcgtgaata caaagagcct
      541 cttaaactcg attgggcaga tgatttaaaa taaaggatct ccaggcatca aataaaacga
      601 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      661 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatag aagaccaagg
      721 aggactttgg caggtgctct cctgagtagg acaaatccgc cgccctagac ctagggtacg
      781 ggttttgctg cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga
      841 tccggcttca gccggtttgc cggctgaaag cactatttct tccagaattg ccatgatttt
      901 ttccccacgg gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagcagc
      961 atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt
     1021 tcaatttcat gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca
     1081 tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa cagctttgaa tgcaccaaaa
     1141 actcgtaaaa gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac
     1201 agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat
     1261 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat
     1321 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata
     1381 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt
     1441 taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt
     1501 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc
     1561 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc
     1621 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca
     1681 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc
     1741 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa
     1801 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata
     1861 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc
     1921 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac
     1981 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac
     2041 accataagca ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga
     2101 taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca
     2161 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc
     2221 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata
     2281 ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat
     2341 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctatac cctctgtaaa
     2401 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat
     2461 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact
     2521 ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa
     2581 cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa
     2641 tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt
     2701 tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat
     2761 ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc
     2821 gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc
     2881 cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct
     2941 aatgcaccca gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctgtgt
     3001 cagatttctt actttattca ggcgattacg tgttacaacc aattaaccaa ttctgattag
     3061 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     3121 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     3181 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     3241 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     3301 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     3361 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     3421 tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     3481 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     3541 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     3601 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     3661 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     3721 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     3781 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctg
     3841 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     3901 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     3961 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     4021 cacctgcccg aaccagagac ttatctcagg acgtcttacg aaaataactc aagctgaata
     4081 acgtgctgca gatggcgggt gaatgcggca tcgtctacct ccggcattcc cagcacgtaa
     4141 atcccgtcca gtccacagac cagcgaaatc agccgccagg cgatattttc ggcgctatcg
     4201 cgcagggtaa attcgccagc ggcatggccc gcgcgaatga tcctgaccgc ttcgtcatgc
     4261 cacaggttca tggtcagcag gtacgcgctt ttgatttccg gatcgctgtc ggccagcagc
     4321 tgcgcctggc gccacaggcg gatatagggc tccagccgtc cgtcctcgct gccgagggcg
     4381 gaaaacagct gttcacgcca gccggcggtg cgcgaaaggc gctgcagatc catcatctcg
     4441 cggatcacgc ggataaacgc ctgggatttt agctcgccgg aggaggtgaa atggtggtgg
     4501 acctggcctg cggcgacgcc cgcggcggtg gcgatgttgc ggaccgtcat accggtaaac
     4561 ccttgatcca gcgcgacgcg catggcggcc tgcatgatgg tttcccgacg ttcttcgcga
     4621 ttcagatagc ccatattgtc acctccttat ccacacatta tacgagccgg atgattaatg
     4681 gggaacctta ccagagggcg ccccagctgg caattcctct taagtaagta agagtatacg
     4741 tatatcggct aataacgtat taaggcgctt cggcgccttt ttttatgggg gtattttcat
     4801 cccaatccac acgtccaacg cacagcaaac accacgtcga ccctatcagc tgcgtgcttt
     4861 ctatgagtcg ttgctgcata ac
//
