LOCUS       Exported                4690 bp ds-DNA     circular UNA 28-MAR-2023
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Escherichia virus T4
  ORGANISM  Escherichia virus T4
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 19, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SnapGene NotesSequence Type: SyntheticConfirmed Experimentally: 
            0Transformed Into: EC100D(TM) pir+SnapGene UUID: 
            56bccd99-cc6d-4a19-8bba-d7cc3a08c40eSequence Document Type: UNAOther
            Notes: CreatedBy: Brady CressImported FromPath: 
            /Users/benjaminadler/Library/CloudStorage/GoogleDrive-ba14@berkeley.
            edu/My Drive/BA_Doudna_Lab/BA_DNA_DB/BA_SnapGene/DNA 
            Files/Phage-based anti-defenseFilename: pBA1399_pJEX-T4acb2_OE.dna
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Escherichia virus T4"
                     /mol_type="genomic DNA"
     source          115..381
                     /organism="Escherichia virus T4"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:10665"
     misc_feature    1..79
                     /label=pJEX-D Promoter
     misc_feature    56..79
                     /label=EilO operator
     primer_bind     complement(72..114)
                     /label=oBA2269_pJEX-SC101Gib_R
     misc_feature    102..107
                     /label=RBS
     primer_bind     112..151
                     /label=oBA2437_T4acb2_OE_F
     misc_feature    113..114
                     /label=2>3
     gene            115..381
                     /gene="vs.4"
                     /locus_tag="T4p115"
                     /label=vs.4
                     /db_xref="GeneID:1258619"
     CDS             115..381
                     /codon_start=1
                     /transl_table=8
                     /gene="vs.4"
                     /locus_tag="T4p115"
                     /product="Vs.4 conserved hypothetical protein"
                     /label=vs.4
                     /db_xref="GeneID:1258619"
                     /protein_id="NP_049728.1"
                     /translation="MIEDIKGYKPHTEEKIGKVNAIKDAEVRLGLIFDALYDEFWEALD
                     NCEDCEFAKNYAESLDQLTIAKTKLKEASMWACRAVFQPEEKY"
     CDS             115..381
                     /codon_start=1
                     /transl_table=0
                     /label=acb2_predicted
                     /translation="MIEDIKGYKPHTEEKIGKVNAIKDAEVRLGLIFDALYDEFWEALD
                     NCEDCEFAKNYAESLDQLTIAKTKLKEASMWACRAVFQPEEKY"
     misc_feature    complement(115..145)
                     /label=T4_acb2_CDS_1
     misc_feature    115..117
                     /label=UP
     misc_feature    complement(160..190)
                     /label=T4_acb2_CDS_2
     primer_bind     complement(352..386)
                     /label=oBA2438_T4acb2_OE_R
     misc_feature    379..381
                     /label=DN
     gene            381
                     /gene="vs.3"
                     /locus_tag="T4p114"
                     /label=vs.3
                     /db_xref="GeneID:1258747"
     primer_bind     382..412
                     /label=oBA2261_express_common_F
     misc_feature    382..385
                     /label=4bp BsaI
     terminator      389..517
                     /label=BBa_B0015 Terminator
     primer_bind     389..420
                     /label=oBFC0174
     primer_bind     389..412
                     /label=oBFC1711
     primer_bind     397..418
                     /label=oBFC0177
     primer_bind     complement(425..468)
                     /label=oBFC0177
     primer_bind     complement(449..475)
                     /label=oBFC1189
     primer_bind     complement(491..517)
                     /label=oBFC0696
     primer_bind     complement(494..518)
                     /label=oBFC0055
     primer_bind     complement(494..518)
                     /label=oBFC0806
     primer_bind     complement(494..517)
                     /label=oBFC0422
     primer_bind     complement(494..517)
                     /label=oBFC0426
     primer_bind     complement(494..517)
                     /label=oBFC0488
     primer_bind     complement(494..517)
                     /label=oBFC0490
     primer_bind     complement(495..538)
                     /label=oBA1653_RFP924_droupout_Gib_R
     primer_bind     complement(496..544)
                     /label=oBA1708_RFPdropout_part4_gib_R
     primer_bind     complement(496..532)
                     /label=oBFC1287
     primer_bind     complement(496..518)
                     /label=oBFC0309
     primer_bind     complement(496..518)
                     /label=oBFC1335
     primer_bind     complement(496..518)
                     /label=oBFC0785
     primer_bind     complement(496..518)
                     /label=oBFC0356
     primer_bind     complement(496..517)
                     /label=oBFC0692
     primer_bind     complement(496..511)
                     /label=oBFC1196
     primer_bind     498..541
                     /label=oBA1650_pBFC0924_Gib_F
     primer_bind     complement(500..518)
                     /label=oBFC1204
     primer_bind     complement(500..518)
                     /label=oBFC0738
     primer_bind     complement(510..538)
                     /label=oBFC1476
     primer_bind     518..567
                     /label=oBA1710_SC101_part4_gib_R
     misc_feature    518..544
                     /label=Pt4_common_region_6>4
     primer_bind     complement(523..545)
                     /label=oBFC1462
     misc_feature    526..529
                     /label=6>4 OH
     misc_feature    complement(530..533)
                     /label=SMRT Gate OH Right
     misc_feature    complement(538..544)
                     /label=AarI
     primer_bind     543..569
                     /label=oBFC0802
     misc_feature    545..2807
                     /label=SC101_part
     primer_bind     545..569
                     /label=oBFC0693
     rep_origin      complement(584..2052)
                     /direction=LEFT
                     /label=SC101
                     /note="/snapgene_direction=LEFT"
     primer_bind     complement(745..768)
                     /label=oBFC0727
     CDS             complement(1102..2052)
                     /codon_start=1
                     /transl_table=0
                     /gene="<i>rep101</i>"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2100..2322
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     complement(2782..2804)
                     /label=oBFC0813a
     primer_bind     complement(2782..2804)
                     /label=oBFC0813
     primer_bind     complement(2788..2837)
                     /label=oBA1709_SC101_part4_gib_F
     misc_feature    2804..2806
                     /label=BsmBI_OH
     primer_bind     2808..2867
                     /label=oBA1718_kanR_part4_gib_R
     misc_feature    2808..2837
                     /label=Part4_Ori_AbR_Common
     terminator      complement(2838..2864)
                     /label=KanR Terminator
     primer_bind     2838..2863
                     /label=oBFC0313
     CDS             complement(2865..3680)
                     /label=KanR
     primer_bind     complement(2949..2976)
                     /label=oBFC0064
     promoter        complement(3681..3828)
                     /label=KanR Promoter
     primer_bind     3829..3882
                     /label=oBA2268_pJEX-SC101Gib_F
     misc_feature    3829..3853
                     /label=Pt4_common_region_4>5
     primer_bind     complement(3829..3853)
                     /label=oBA2260_pt4_common_4>5_R
     misc_feature    3829..3835
                     /label=AarI
     primer_bind     complement(3835..3853)
                     /label=oBA1651_pBFC0924_Gib_R
     misc_feature    3840..3843
                     /label=SMRT Gate OH Left
     misc_feature    3844..3845
                     /label=spacer in case extra trimming during AarI GG
     misc_feature    3846..3849
                     /label=pBBR1 Backbone Index (GACT)
     misc_feature    3850..3853
                     /label=4>5 OH
                     /label=4b(3)
     misc_feature    3850..3853
                     /label=BsaI OH
     misc_feature    3854..4804
                     /label=from gbBA189
     misc_feature    3854..3857
                     /label=1>2
     CDS             complement(3864..4442)
                     /codon_start=1
                     /transl_table=0
                     /label=eilR
                     /translation="MGYLNREERRETIMQAAMRVALDQGFTGMTVRNIATAAGVAAGQV
                     HHHFTSSGELKSQAFIRVIREMMDLQRLSRTAGWREQLFSALGSEDGRLEPYIRLWRQA
                     QLLADSDPEIKSAYLLTMNLWHDEAVRIIRAGHAAGEFTLRDSAENIAWRLISLVCGLD
                     GIYVLGMPEVDDAAFTRHLQHVIQLELFS"
     misc_feature    3900
                     /label=AarI removal
     misc_feature    4443..4457
                     /label=GSG_RBS
     RBS             4449..4457
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in <i>E. coli</i>; complementary 
                     to a region in the 3' end of the 16S rRNA (<a 
                     href=""https://www.ncbi.nlm.nih.gov/pubmed/7528374"" 
                     title=""https://www.ncbi.nlm.nih.gov/pubmed/7528374"">Chen 
                     et al., 1994</a>)"
     misc_feature    complement(4465..4493)
                     /label=pFAB weak constitutive
ORIGIN
        1 aaaaaactgc aaaaaatagt ttgacaggac acgtgtccaa ctttaagatg tacccaaagt
       61 tggacacgtg tccaactttg aattcaaaag atcttttaag aaggagatat actgatgatt
      121 gaagatatta agggttataa accacatact gaagagaaaa tcggtaaagt aaatgctatt
      181 aaagacgctg aagttcgttt aggacttatc tttgatgctt tatatgatga attctgggaa
      241 gcactagata attgcgaaga ctgtgaattc gcgaagaatt atgctgaaag tctcgatcag
      301 ttaactattg ctaaaacgaa actcaaagaa gccagtatgt gggcttgtcg tgcagtgttc
      361 caaccagagg aaaaatacta aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg
      421 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      481 actggctcac cttcgggtgg gcctttctgc gtttatagaa gaccaaggag gactttggca
      541 ggtgctctcc tgagtaggac aaatccgccg ccctagacct agggtacggg ttttgctgcc
      601 cgcaaacggg ctgttctggt gttgctagtt tgttatcaga atcgcagatc cggcttcagc
      661 cggtttgccg gctgaaagca ctatttcttc cagaattgcc atgatttttt ccccacggga
      721 ggcgtcactg gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc
      781 aggctgtcta tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt
      841 tctagttgct ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct
      901 gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc
      961 tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt
     1021 gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata
     1081 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt
     1141 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat
     1201 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg
     1261 tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg ttggtatttt
     1321 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc
     1381 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt
     1441 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct
     1501 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat
     1561 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat
     1621 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt
     1681 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct
     1741 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct
     1801 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt
     1861 ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc
     1921 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt
     1981 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac
     2041 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg
     2101 ggctagtcaa tgataattac tagtcctttt cctttgagtt gtgggtatct gtaaattctg
     2161 ctagaccttt gctggaaaac ttgtaaattc tgctataccc tctgtaaatt ccgctagacc
     2221 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata
     2281 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc
     2341 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa
     2401 ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt
     2461 ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca
     2521 cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa
     2581 ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg
     2641 gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc
     2701 cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt
     2761 aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctgtgtca gatttcttac
     2821 tttattcagg cgattacgtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
     2881 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     2941 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     3001 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     3061 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     3121 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     3181 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgaggcga
     3241 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     3301 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     3361 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     3421 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     3481 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     3541 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     3601 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctgga gcaagacgtt
     3661 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     3721 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     3781 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagca cctgcccgaa
     3841 ccagagactt atctcaggac gtcttacgaa aataactcaa gctgaataac gtgctgcaga
     3901 tggcgggtga atgcggcatc gtctacctcc ggcattccca gcacgtaaat cccgtccagt
     3961 ccacagacca gcgaaatcag ccgccaggcg atattttcgg cgctatcgcg cagggtaaat
     4021 tcgccagcgg catggcccgc gcgaatgatc ctgaccgctt cgtcatgcca caggttcatg
     4081 gtcagcaggt acgcgctttt gatttccgga tcgctgtcgg ccagcagctg cgcctggcgc
     4141 cacaggcgga tatagggctc cagccgtccg tcctcgctgc cgagggcgga aaacagctgt
     4201 tcacgccagc cggcggtgcg cgaaaggcgc tgcagatcca tcatctcgcg gatcacgcgg
     4261 ataaacgcct gggattttag ctcgccggag gaggtgaaat ggtggtggac ctggcctgcg
     4321 gcgacgcccg cggcggtggc gatgttgcgg accgtcatac cggtaaaccc ttgatccagc
     4381 gcgacgcgca tggcggcctg catgatggtt tcccgacgtt cttcgcgatt cagatagccc
     4441 atattgtcac ctccttatcc acacattata cgagccggat gattaatggg gaaccttacc
     4501 agagggcgcc ccagctggca attcctctta agtaagtaag agtatacgta tatcggctaa
     4561 taacgtatta aggcgcttcg gcgccttttt ttatgggggt attttcatcc caatccacac
     4621 gtccaacgca cagcaaacac cacgtcgacc ctatcagctg cgtgctttct atgagtcgtt
     4681 gctgcataac
//
