LOCUS       Exported                4031 bp ds-DNA     circular SYN 08-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 19, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SnapGene NotesSequence Type: NaturalTransformed Into: 
            unspecifiedSnapGene UUID: 
            25008e45-0d74-4aba-9b96-32df478c2467Sequence Document Type: 
            UNAImported FromPath: 
            /Users/benjaminadler/Library/CloudStorage/GoogleDrive-ba14@berkeley.
            edu/My Drive/BA_Doudna_Lab/BA_DNA_DB/BA_SnapGene/DNA 
            Files/Phage_Immune_SystemsFilename: 
            pBA1729_pTet__mCpolTA_ECOR31_STANDARD.dna
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    7..633
                     /label=tetR
     misc_feature    634..706
                     /label=tet promoter region
     misc_feature    655..673
                     /label=Q1 operator (tetR)
     misc_feature    655..673
                     /label=Q2 operator (TetR )
     primer_bind     complement(693..746)
                     /label=oBA1120_GG_pBA368_Gib_F
     primer_bind     complement(693..746)
                     /label=oBA1120_GG_pBA368_Gib_Fa
     misc_feature    722..741
                     /label=RBS(JBEI biobricks)
     primer_bind     737..788
                     /label=oBA2892_pTet_mCpol_ECOR31_GG_F
     misc_feature    738..741
                     /label=UP
     misc_feature    742..2163
                     /label=ECOR31_mCpol_Native_STANDARD
     CDS             1042..1401
                     /codon_start=1
                     /transl_table=0
                     /label=mCpol-like
                     /translation="MYIAIDGDDVGRKITSSYLSNSEERLTYISNKLNDTTKKISKMLL
                     SNGFEIIFQAADGVTAKTDNEVNLNFVFDKIKSYSFDEITFSAGVGANLREAYVALLNS
                     KSNGKNMISIYKDIL"
     primer_bind     1042..1070
                     /label=oBA3020__pJEX_mCpol_ECOR31_solo_F
     primer_bind     complement(1130..1171)
                     /label=oBA2350_ECOR31_mCpol_UNK_Native_Gib_p1_R
     primer_bind     1130..1171
                     /label=oBA2351_ECOR31_mCpol_UNK_Native_Gib_p2_F
     misc_feature    1152..1159
                     /label=Problem_region_ECOR31
     CDS             1411..2013
                     /codon_start=1
                     /transl_table=0
                     
                     /translation="MFRIIRFDWLLKTVSLISLFVTILSFWIFKTVLSSDLSLLKLLSI
                     SSLASIIINFFLLSSKISRLIWGTIRFFKKDIYPDLNGIWVGHIITETNDEFEVRAKIK
                     QALLVTKIEMHGPTVKSVTLEATPTKELDNNKLYYVYKSTPKNPSWSEYIGSTIFDVIE
                     SNNALQLSGRYYTDRKSVGRISIKRISLNTDSDISFY"
     primer_bind     complement(1970..2013)
                     /label=oBA2598_mCpol_2TMbeta_BsaI_R
     primer_bind     complement(2128..2168)
                     /label=oBA2893_pTet_mCpol_ECOR31_GG_R
     primer_bind     2164..2193
                     /label=oBA1121_GG_pBA368_Gib_R
     primer_bind     2164..2193
                     /label=oBA1121_GG_pBA368_Gib_Ra
     misc_feature    2164..2167
                     /label=DN
     misc_feature    2188..2316
                     /label=dbI terminator
     misc_feature    2323..3034
                     /label=p15A
     misc_feature    3125..3230
                     /label=To (terminator ?)
     misc_feature    3246..3905
                     /label=cmR2
ORIGIN
        1 gacgtcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaagatc
      721 ttttaagaag gagatataca ttgagaaatc tttagtgtaa agatttaata attatccttt
      781 aagtgtagat tacaagcaga acaattgaat taatcgccac attgaaaata taaaccctca
      841 acacacttga tgtatttaac aagacatagg attaaaccca ttaaaattaa aaaaatttaa
      901 aatacaaacc tcgttttggc cgggtataaa tacaatttaa accgcaacgt ttttaagtaa
      961 ataaattcat ttgcaactaa gcattcaggg ttgatataat ataaccactt agataataat
     1021 ccatgttagt ttggagttaa tatgtacata gcgatagatg gcgatgatgt ggggagaaaa
     1081 ataacctcat catacttatc caactcagaa gagaggttga catacataag caacaaatta
     1141 aatgatacca caaaaaaaat atctaaaatg cttttatcca atggatttga gataattttc
     1201 caagccgcag atggtgttac agcgaaaaca gacaacgagg tcaatcttaa ctttgttttt
     1261 gataagataa aatcctattc ctttgacgaa attacatttt cagctggtgt gggagcgaat
     1321 ctaagagaag cctatgttgc actgttaaat tctaaaagca acggtaagaa tatgatttct
     1381 atttacaaag acatattata atggtgcgaa atgtttagaa taataagatt tgactggttg
     1441 ttaaaaacgg tatcactaat ctctttgttt gtcacaatac tttcattttg gatctttaag
     1501 acagttctgt cttctgactt aagcctgctc aaattgttat caatatcatc attagcatcc
     1561 attatcataa acttctttct cctttcatca aagatatcaa gactaatatg gggaacaata
     1621 aggtttttca agaaagacat atatcctgac ttaaatggaa tttgggttgg acatataatc
     1681 acagaaacaa atgatgaatt tgaagtccgc gcaaaaataa agcaagcact ccttgtaaca
     1741 aagatagaaa tgcacggccc cacagttaaa tctgttacgc tagaagcaac gccaactaag
     1801 gaactggata acaacaaatt gtattatgta tataaatcaa cccctaaaaa ccctagttgg
     1861 agcgagtata ttggctcgac tatattcgac gtaattgaaa gtaacaatgc gctccaactt
     1921 tccggtcgat attatacaga cagaaaatcc gtggggagaa tatcaataaa aagaatatca
     1981 cttaacactg actccgatat atctttttat taactttcgg aatgtttaat taccacaatc
     2041 agccatgttc gttcttttaa aatagaagga taattcgcgc tactgatata ttaatcatgg
     2101 aaaaactcct ttcagtagcc attttcccta gaaaaatacc cctgcatacc tcatatactc
     2161 catggatcca aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
     2221 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2281 ctggctcacc ttcgggtggg cctttctgcg tttataccta gggatatatt ccgcttcctc
     2341 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     2401 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     2461 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     2521 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     2581 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     2641 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     2701 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     2761 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     2821 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     2881 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     2941 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     3001 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
     3061 tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgttac
     3121 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     3181 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     3241 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     3301 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     3361 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     3421 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     3481 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     3541 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     3601 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     3661 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     3721 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     3781 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     3841 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     3901 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     3961 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     4021 cgccagatat c
//
