LOCUS       Exported                2969 bp ds-DNA     linear   SYN 13-JUL-2024
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 19, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SnapGene NotesSequence Type: NaturalTransformed Into: 
            unspecifiedSnapGene UUID: 
            70c2eb88-dade-4634-ad74-c485a4584525Sequence Document Type: 
            UNAImported FromPath: 
            /Users/benjaminadler/Library/CloudStorage/GoogleDrive-ba14@berkeley.
            edu/My Drive/BA_Doudna_Lab/BA_DNA_DB/BA_SnapGene/DNA 
            Files/Phage_Immune_SystemsFilename: 
            pBA1739_pTet__mCpol_ECOR31_solo.dna
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    7..633
                     /label=tetR
     misc_feature    634..706
                     /label=tet promoter region
     misc_feature    655..673
                     /label=Q1 operator (tetR)
     misc_feature    655..673
                     /label=Q2 operator (TetR )
     primer_bind     complement(693..741)
                     /label=oBA1120_GG_pBA368_Gib_F
     primer_bind     complement(693..741)
                     /label=oBA1120_GG_pBA368_Gib_Fa
     misc_feature    722..741
                     /label=RBS(JBEI biobricks)
     primer_bind     737..770
                     /label=oBA2976_ECOR31_mCpol_mCpol_BsaI_F_v2
     misc_feature    738..741
                     /label=UP
     misc_feature    742..1101
                     /label=ECOR31_mCpol_Native_STANDARD
     CDS             742..1101
                     /codon_start=1
                     /transl_table=0
                     /label=mCpol-like
                     /translation="MYIAIDGDDVGRKITSSYLSNSEERLTYISNKLNDTTKKISKMLL
                     SNGFEIIFQAADGVTAKTDNEVNLNFVFDKIKSYSFDEITFSAGVGANLREAYVALLNS
                     KSNGKNMISIYKDIL"
     primer_bind     742..770
                     /label=oBA3020__pJEX_mCpol_ECOR31_solo_F
     primer_bind     complement(830..871)
                     /label=oBA2350_ECOR31_mCpol_UNK_Native_Gib_p1_R
     primer_bind     830..871
                     /label=oBA2351_ECOR31_mCpol_UNK_Native_Gib_p2_F
     misc_feature    852..859
                     /label=Problem_region_ECOR31
     primer_bind     complement(1058..1106)
                     /label=oBA2977_ECOR31_mCpol_mCpol_BsaI_R_v2
     primer_bind     complement(1058..1101)
                     /label=oBA3021__pJEX_mCpol_ECOR31_solo_R
     primer_bind     1101..1131
                     /label=oBA1121_GG_pBA368_Gib_R
     primer_bind     1101..1131
                     /label=oBA1121_GG_pBA368_Gib_Ra
     misc_feature    1102..1105
                     /label=DN
     misc_feature    1126..1254
                     /label=dbI terminator
     misc_feature    1261..1972
                     /label=p15A
     misc_feature    2063..2168
                     /label=To (terminator ?)
     misc_feature    2184..2843
                     /label=cmR2
ORIGIN
        1 gacgtcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaagatc
      721 ttttaagaag gagatataca tatgtacata gcgatagatg gcgatgatgt ggggagaaaa
      781 ataacctcat catacttatc caactcagaa gagaggttga catacataag caacaaatta
      841 aatgatacca caaaaaaaat atctaaaatg cttttatcca atggatttga gataattttc
      901 caagccgcag atggtgttac agcgaaaaca gacaacgagg tcaatcttaa ctttgttttt
      961 gataagataa aatcctattc ctttgacgaa attacatttt cagctggtgt gggagcgaat
     1021 ctaagagaag cctatgttgc actgttaaat tctaaaagca acggtaagaa tatgatttct
     1081 atttacaaag acatattata aggatccaaa ctcgagtaag gatctccagg catcaaataa
     1141 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1201 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatacctagg
     1261 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     1321 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     1381 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     1441 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     1501 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     1561 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     1621 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     1681 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
     1741 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
     1801 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     1861 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
     1921 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
     1981 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
     2041 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     2101 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     2161 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     2221 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     2281 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     2341 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     2401 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     2461 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     2521 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     2581 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     2641 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     2701 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     2761 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     2821 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     2881 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     2941 cgatcaacgt ctcattttcg ccagatatc
//
