LOCUS       Exported                7540 bp ds-DNA     circular SYN 18-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Doudna Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 19, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(28..73)
                     /label=ED_pr_471
     RBS             58..63
                     /note="ribosome binding site"
     misc_feature    69..112
                     /label=MCS-1
                     /note="multiple cloning site #1"
     CDS             71..73
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /translation="M"
     primer_bind     complement(78..115)
                     /label=ED_pr_285
     primer_bind     complement(79..100)
                     /label=ED_pr_269
     CDS             83..100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     86..121
                     /label=ED_pr_284
     misc_feature    86..115
                     /label=Gibson OH
     primer_bind     complement(86..106)
                     /label=Primer 2
     CDS             113..367
                     /codon_start=1
                     /label=hSUMO2
                     /translation="GVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGL
                     SMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     misc_feature    341..370
                     /label=OH
     misc_feature    350..370
                     /label=Gibson homology arm
     primer_bind     complement(352..400)
                     /label=ED_pr_371
     primer_bind     complement(352..370)
                     /label=ED_pr_395
     misc_feature    368..370
                     /label=Serine linker
     CDS             371..757
                     /codon_start=1
                     
                     /translation="FKKDIYPDLNGIWVGHIITETNDEFEVRAKIKQALLVTKIEMHGP
                     TVKSVTLEATPTKELDNNKLYYVYKSTPKNPSWSEYIGSTIFDVIESNNALQLSGRYYT
                     DRKSVGRISIKRISLNTDSDISFY"
     primer_bind     371..430
                     /label=ED_pr_372
     primer_bind     371..409
                     /label=ED_pr_396
     primer_bind     371..409
                     /label=ED_pr_470
     misc_feature    371..400
                     /label=Gibson OH
     CDS             374..394
                     /codon_start=1
                     
                     /translation="KKDIYPD"
     misc_feature    386..389
                     /label=OH GGGA
     primer_bind     complement(713..754)
                     /label=ED_pr_467
     primer_bind     complement(716..787)
                     /label=ED_pr_279
     primer_bind     complement(734..757)
                     /label=ED_pr_210
     primer_bind     755..775
                     /label=ED_pr_468
     misc_feature    758..787
                     /label=OH
     misc_feature    758..780
                     /label=MCS-1
                     /note="multiple cloning site #1"
     primer_bind     758..775
                     /label=ED_pr_278
     primer_bind     801..820
                     /label=DuetUP2 Primer
     primer_bind     complement(801..820)
                     /label=DuetDOWN1 Primer
     promoter        826..844
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    845..869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(896..944)
                     /label=ED_pr_280
     RBS             898..903
                     /note="ribosome binding site"
     misc_feature    909..928
                     /label=MCS-2
                     /note="multiple cloning site #2"
     CDS             912..914
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /translation="M"
     primer_bind     915..962
                     /label=ED_pr_281
     misc_feature    915..944
                     /label=OH2
     primer_bind     921..959
                     /label=ED_pr_253
     CDS             930..2030
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1386..1405
                     /label=HS_2
     primer_bind     2004..2027
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     primer_bind     complement(2072..2101)
                     /label=HS_85
     primer_bind     2082..2136
                     /label=oBA2463_ECOR31mCpol_mCpol-sys_purification_F
     CDS             2085..2105
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     misc_feature    2087..2111
                     /label=Gibson_UP
     CDS             2112..2468
                     /codon_start=1
                     /label=mCpol Domain Protein
                     /translation="MYIAIDGDDVGRKITSSYLSNSEERLTYISNKLNDTTKKISKMLL
                     SNGFEIIFQAADGVTAKTDNEVNLNFVFDKIKSYSFDEITFSAGVGANLREAYVALLNS
                     KSNGKNMISIYKDIL"
     primer_bind     2112..2137
                     /label=Test
     primer_bind     2112..2136
                     /label=oBA2450_gbBA211_Gib_F
     misc_feature    2222..2229
                     /label=Problematic PolyA
     primer_bind     complement(2422..2486)
                     /label=ED_pr_282
     primer_bind     complement(2426..2471)
                     /label=Primer 1
     primer_bind     complement(2428..2480)
                     /label=ED_pr_254
     primer_bind     complement(2433..2471)
                     /label=oBA2464_ECOR31mCpol_mCpol-sys_purification_R
     primer_bind     2457..2494
                     /label=ED_pr_283
     misc_feature    2457..2486
                     /label=OH
     misc_feature    2472..2558
                     /label=MCS-2
                     /note="multiple cloning site #2"
     CDS             2486..2530
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(2568..2586)
                     /label=T7 Terminator Primer
     terminator      2582..2629
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2666..3121
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(3236..4096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4097..4189)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4270..4858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5094..5096
                     /label=3 bp deletion in ED_p_102
     CDS             complement(5288..5479)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5299..5311)
                     /label=Primer 2
     CDS             complement(6051..7133)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(7134..7211)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7478..7493
                     /label=pET Upstream Primer
     promoter        join(7524..7540,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgggagtcaa
      121 gactgagaac aacgatcata ttaatttgaa ggtggcgggg caggatggtt ctgtggtgca
      181 gtttaagatt aagaggcata caccacttag taaactaatg aaagcctatt gtgaacgaca
      241 gggattgtca atgaggcaga tcagattccg atttgacggg caaccaatca atgaaacaga
      301 cacacctgca cagttggaaa tggaggatga agatacaatt gatgtgtttc aacagcagac
      361 gggtggatca ttcaagaaag acatatatcc tgacttaaat ggaatttggg ttggacatat
      421 aatcacagaa acaaatgatg aatttgaagt ccgcgcaaaa ataaagcaag cactccttgt
      481 aacaaagata gaaatgcacg gccccacagt taaatctgtt acgctagaag caacgccaac
      541 taaggaactg gataacaaca aattgtatta tgtatataaa tcaaccccta aaaaccctag
      601 ttggagcgag tatattggct cgactatatt cgacgtaatt gaaagtaaca atgcgctcca
      661 actttccggt cgatattata cagacagaaa atccgtgggg agaatatcaa taaaaagaat
      721 atcacttaac actgactccg atatatcttt ttattaactt gcggccgcat aatgcttaag
      781 tcgaacagaa agtaatcgta ttgtacacgg ccgcataatc gaaattaata cgactcacta
      841 taggggaatt gtgagcggat aacaattccc catcttagta tattagttaa gtataagaag
      901 gagatataca tatggcagat ctcaattgta tgaaaatcga agaaggtaaa ctggtaatct
      961 ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag
     1021 ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg
     1081 ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct
     1141 acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt
     1201 atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg
     1261 ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg
     1321 aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca
     1381 acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca
     1441 agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag
     1501 cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt
     1561 actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt
     1621 gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct
     1681 tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca
     1741 gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc
     1801 tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag
     1861 agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca
     1921 tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg
     1981 ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct
     2041 cgaacaacaa caacaataac aataacaaca acctcgggat cgaggaaaac ctgtacttcc
     2101 aatccaatgc aatgtacata gcgatagatg gcgatgatgt ggggagaaaa ataacctcat
     2161 catacttatc caactcagaa gagaggttga catacataag caacaaatta aatgatacca
     2221 caaaaaaaat atctaaaatg cttttatcca atggatttga gataattttc caagccgcag
     2281 atggtgttac agcgaaaaca gacaacgagg tcaatcttaa ctttgttttt gataagataa
     2341 aatcctattc ctttgacgaa attacatttt cagctggtgt gggagcgaat ctaagagaag
     2401 cctatgttgc actgttaaat tctaaaagca acggtaagaa tatgatttct atttacaaag
     2461 acatattata accctcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca
     2521 catggactcg tctactagcg cagcttaatt aacctaggct gctgccaccg ctgagcaata
     2581 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     2641 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2701 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2761 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2821 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2881 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2941 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3001 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3061 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     3121 tctggcggca cgatggcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3181 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3241 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3301 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3361 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3421 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3481 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3541 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3601 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3661 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3721 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3781 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3841 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3901 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3961 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4021 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4081 aatgttgaat actcatactc ttcctttttc aatcatgatt gaagcattta tcagggttat
     4141 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggtcatgac
     4201 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4261 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4321 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4381 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     4441 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4501 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4561 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4621 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4681 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4741 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4801 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4861 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4921 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4981 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5041 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     5101 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     5161 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     5221 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5281 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     5341 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     5401 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     5461 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     5521 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     5581 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     5641 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     5701 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     5761 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     5821 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     5881 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgctag
     5941 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     6001 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
     6061 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6121 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     6181 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     6241 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     6301 tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag cccggactcg
     6361 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     6421 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     6481 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     6541 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     6601 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     6661 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     6721 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     6781 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     6841 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     6901 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     6961 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     7021 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     7081 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     7141 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     7201 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     7261 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     7321 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     7381 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     7441 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
     7501 gatcgatctc gatcccgcga aattaatacg actcactata
//
