LOCUS       pEMS2393                7562 bp ds-DNA     circular     07-JAN-2026
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             670..1527
                     /label="Amp-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1701..2289
                     /label="Origin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     repeat_region   2718..2885
                     /label="5'\ITR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2914..2925
                     /label="MCS\5'\end"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2918..2925
                     /label="FseI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2926..4452
                     /label="RR9\(SIMOmod)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2926..4906
                     /label="Ple331"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3179..3198)
                     /label="oEMS6113"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3233..3252)
                     /label="oEMS6114"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3855..3874
                     /label="oEMS6116"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4453..4906
                     /label="P0"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4584..4603)
                     /label="oEMS6117"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4907..4914
                     /label="AscI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     intron          4915..5087
                     /label="Intron"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    5096..6442
                     /label="3xFLAG/PAX6"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(5102..5121)
                     /label="oEMS6143"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(5149..5168)
                     /label="oEMS6144"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(5195..5214)
                     /label="Endo-Pax6 Rev"
                     /note="sequence: tgacaaagacaccaccgagc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(5370..5392)
                     /label="oEMS5948"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          5675..5694
                     /label="PAX6 forw"
                     /note="sequence: gcaacctacgcaagatggct"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(5750..5772)
                     /label="PAX6 rev"
                     /note="sequence: gcatttgagcctcatctgaatct"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     enhancer        6449..7035
                     /label="WPRE"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     6526..6550
                     /label="oEMS5939"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    7294..7339
                     /label="additional\AAV\sequences"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(7302..7469)
                     /label="3'\ITR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
       61 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      121 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      181 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      241 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      301 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      361 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
      421 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
      481 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
      541 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      601 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      661 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      721 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      781 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      841 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      901 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      961 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1021 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1081 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1141 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1201 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1261 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1321 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1381 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1441 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1501 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1561 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1621 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2281 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2401 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2461 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2521 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     2581 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     2641 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     2701 gccagattta attaaggctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg
     2761 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
     2821 tggccaactc catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat
     2881 ctacgtagcc atgctctagg aagatcggaa ttccctaggc cggcctccct tttgctacag
     2941 gcatagttta agtcttgtgt cacaaataaa aggatcctcc ctagcatatg gagtagttgc
     3001 catgtcctcc cagggatggt ctgtagctat gttcacctca ggctcatcaa gaagaggtaa
     3061 actgtcaagt ataatttcag tcatagggtg gtttggttga gacagattag tggtatgcaa
     3121 tcatgataga tagtttatcc cagctccaaa ctgccgcttg ggaaatcttg aactcaaatg
     3181 aacagagact cagtcattat tagtgtcaaa aaggtataca gaggcaaaat ttggttggca
     3241 gcataaaaga tgaatcaaag caaaaaagtg accaattgca tgttcaaaat cgaaattgag
     3301 atgcatacta cagcttggaa atactttaaa taccagaaaa gtcacacatt attaattgcg
     3361 cattgattaa tatttaaaac atgtttctgt aatgtatttt caaagagaca ataaaattac
     3421 atgtattttc ttatttcaaa acctttattc tcaaggtgat gcatagtaag gagtaccctt
     3481 ttagatacag gagtaagata gcaaaatgtt ttttaaaaat ttgtttaatt tttagtatct
     3541 ttccttcagc tgcatatttc cagaaataag actttttaga agaagaattt agttttatgg
     3601 gactatttta atattcaaat gtcttacagt ttgatgactt tctcaggaag aaatcaggtt
     3661 ataataaaag catgaattta aaatgtggaa gaacctaatc taaataactt tccaaatatt
     3721 ttctaaacac tgaaaaatat atatttataa attgtaaaat acattattct attagcaaac
     3781 acaaactttg gtatctttaa atcagactcc ccccccaacc aaatatccac aaaaagaatg
     3841 tcctcaagtg tcaatgcatg aagtcatgcg tgaagataaa tctaacagct ctttctgtgt
     3901 cattcctaat gcacttgtca ctcagtatta tccatcctca ttaatgacaa tgggaaagtt
     3961 tatcttggga acacgtttta aatcatctac tctttacaat gggaccatga aatctcttac
     4021 aaaacatgag gcgggaacta ctctgacaac aaaacccctt cctggcagct ttattcttaa
     4081 ttcctgttca aggagtaatc tgtttattat aaaatcatac agaacaatat attaaacaga
     4141 atttgaaaca agaatagcta gtgtgatttc caagcttaca agtattttta caacattttt
     4201 attaggctga aaacactaca ttgagttctt tttgtgtgat ttcatgcaat cttctattct
     4261 acatcgtaaa cacctttgaa aagaagtttt gttttacata atttaaaaat aattttcaaa
     4321 tggtaaggac actggtgttt atataattat tttaaaatac aataacttac attagcttaa
     4381 ataaaagcag ttattaagta aaaatgtgct catgtttaaa tttcctaacc aaaatgccaa
     4441 aatattccaa ctcttgggga tcggaggagg ggacagggtg attacccaga gaggtagctg
     4501 gccagcctaa gggcagagat cttggggccc tagtgcccga aggtgcggag gagcgcactc
     4561 ggcaagacta gtttcctggg gatcgactct acgccataca ggacggcggc ccaggctgga
     4621 ccgggccggg ctagagcagt cacaggccgg gccaaggaag gccaaagcag gggttggagc
     4681 cggccggacc ctgggtgggg agaagcaggc tcccgcccgg ccggaaaact agtcggcgca
     4741 gagctgtgcc caactctagc cgccatgacg tcacgcgggc cgggcagcca atgaggacgg
     4801 cgctggcgtg gatattaagg aaagttagcg cctgcctgag caccctcttt tcttatcatt
     4861 gacatttaaa ctctggggca ggtcctcgcg tagaacgcgg ctgtcaggcg cgccgaagtt
     4921 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     4981 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     5041 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccaggcg gccgccacca
     5101 tggactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
     5161 atgacaagca gaacagtcac agcggagtga atcagctcgg tggtgtcttt gtcaacgggc
     5221 ggccactgcc ggactccacc cggcagaaga ttgtagagct agctcacagc ggggcccggc
     5281 cgtgcgacat ttcccgaatt ctgcaggtgt ccaacggatg tgtgagtaaa attctgggca
     5341 ggtattacga gactggctcc atcagaccca gggcaatcgg tggtagtaaa ccgagagtag
     5401 cgactccaga agttgtaagc aaaatagccc agtataagcg ggagtgcccg tccatctttg
     5461 cttgggaaat ccgagacaga ttactgtccg agggggtctg taccaacgat aacataccaa
     5521 gcgtgtcatc aataaacaga gttcttcgca acctggctag cgaaaagcaa cagatgggcg
     5581 cagacggcat gtatgataaa ctaaggatgt tgaacgggca gaccggaagc tggggcaccc
     5641 gccctggttg gtatccgggg acttcggtgc cagggcaacc tacgcaagat ggctgccagc
     5701 aacaggaagg agggggagag aataccaact ccatcagttc caacggagaa gattcagatg
     5761 aggctcaaat gcgacttcag ctgaagcgga agctgcaaag aaatagaaca tcctttaccc
     5821 aagagcaaat tgaggccctg gagaaagagt ttgagagaac ccattatcca gatgtgtttg
     5881 cccgagaaag actagcagcc aaaatagatc tacctgaagc aagaatacag gtatggtttt
     5941 ctaatcgaag ggccaaatgg agaagagaag aaaaactgag gaatcagaga agacaggcca
     6001 gcaacacacc tagtcatatt cctatcagca gtagtttcag caccagtgtc taccaaccaa
     6061 ttccacaacc caccacaccg gtttcctcct tcacatctgg ctccatgttg ggccgaacag
     6121 acacagccct cacaaacacc tacagcgctc tgccgcctat gcccagcttc accatggcaa
     6181 ataacctgcc tatgcaaccc ccagtcccca gccagacctc ctcatactcc tgcatgctgc
     6241 ccaccagccc ttcggtgaat gggcggagtt atgataccta caccccccca catatgcaga
     6301 cacacatgaa cagtcagcca atgggcacct cgggcaccac ttcaacagga ctcatttccc
     6361 ctggtgtgtc agttccagtt caagttcccg gaagtgaacc tgatatgtct caatactggc
     6421 caagattaca gtaagcggcc gcgatcgctc aacctctgga ttacaaaatt tgtgaaagat
     6481 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6541 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6601 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6661 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6721 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6781 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6841 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6901 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6961 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7021 gggccgcctc cccgcgatcg cagacatgat aagatacatt gatgagtttg gacaaaccac
     7081 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     7141 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     7201 tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     7261 tagtcgaaat tcccgataag gatcttccta gagcatggct acgtagataa gtagcatggc
     7321 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     7381 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     7441 gcggcctcag tgagcgagcg agcgcgcagc cttaattaac ctaattcact ggccgtcgtt
     7501 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7561 cc
//