LOCUS       Exported                7149 bp ds-DNA     circular SYN 15-AUG-2025
DEFINITION  24.JN_1-54.
ACCESSION   24.JN_1-54
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        400..676
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          677..1697
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.4"
                     /note="/other=intron"
     CDS             1695..1751
                     /label=HBB_LEPEU (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=94.7"
                     /note="/match_length=12.9"
                     /note="/other=CDS"
     CDS             1830..4151
                     /codon_start=1
                     /product="https://www.ncbi.nlm.nih.gov/nucleotide/NM_139038
                     .2"
                     /label=Rin1_NM_139038.2
                     /label=RIN1_MOUSE
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=92.4"
                     /note="/match_length=101.4"
                     /note="/other=CDS"
                     /translation="MEDPGETEAHPLGATSLNFVPGYQQEEKPSPDPLYDTPDARGVQA
                     GGSQQPARTVSLRERLLITRPVWLQLRANAAAALHVLRTEPPGTFLVRKSNTRQCQTLC
                     VRLPEASGPSFVSSHYLQESPGGISLEGSELTFPDLVQLICAYCHTRDILLLPLRLPRA
                     IHQAATHKELEAISHLGMEFWSSSLNTKNQQRPSEAPQIPRLKARSPQELDQGTGAALC
                     FFNPLFPGDLGPTKREKFKRSFKVRVSTETSSPLSPPAVPPPPVPVLPGTSSSQTERLP
                     PRQLLQRESSVGYRVPGSASGPSLPPLPSLQEVDCCSPSSSEEEGSSGSPTTSPRLSRP
                     RHRRPLLRSMSSAFCSLLAPERQVGRAATMLMQNRYTAVGQLVQDLLTQVRVGPESREL
                     QGIRQALSRARAMLSAELGPEKLLPPERLELVLEKSLHRSVLKPLRPILAARLRRRLST
                     DGSLGRLAEGFRLARTQGPGAFGSHLNLSSPVEIEPVRQKLLQLLRAYSPSAQIKWLLQ
                     ACKLLYTALKTQAGENAGADEFLPLLSLVLAQCDLPDLLLEAEYMSELLEPTLLTGEGG
                     YYLTSLSASLALLSGLSQAHALPLSPAQELQRSLALWEQRRLPATHNFQHLLRVAYQDP
                     STGCTSKTLAVPPGSSIATLSQLCATKFRVTQPDAFGLFLYKDQGYHRLPPEALVHRLP
                     TTGYLIYRRAERPETQRAATEKTKTGNERPEAGAWEEEKGGLNGEGKSEIAVDQEGKDQ
                     ARGGHMQLEEQKAEGCPALEE"
     polyA_signal    4246..4301
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     primer_bind     complement(4662..4678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4686..4702
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        4710..4740
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    4755..4776
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(4789..4828)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     promoter        4835..5030
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4835..4857
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     rep_origin      4881..5016
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     polyA_signal    5036..5170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5036..5157
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(5172..5226)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     repeat_region   5238..5249
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=itrs"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.3"
                     /note="/other=repeat_region"
     rep_origin      5409..5997
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(6168..7028)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        7029..7133
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
      481 aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccag gcggggcggg
      541 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      601 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      661 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
      721 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
      781 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
      841 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
      901 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
      961 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1021 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     1081 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     1141 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     1201 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     1261 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     1321 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     1381 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     1441 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     1501 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     1561 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     1621 cggcttctgg cgtgtgaccg gcggctctag ctagagcctc tgctaaccat gttcatgcct
     1681 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     1741 gcaaagaatt cgccaccatg tgcacgcctg tgccttgggg gttggattct cttcctgagt
     1801 cccaggagaa gctgaaggag ctcccagcca tggaagaccc tggtgagacc gaagcacacc
     1861 ctctgggagc caccagcctg aactttgtac ctgggtacca acaggaagaa aagccatctc
     1921 cagacccact gtatgacaca cctgatgcca gaggggttca ggcaggcggg tcccaacaac
     1981 cagcacgcac agtgagcttg cgggagcggc tgctgatcac ccggcctgtg tggttacagc
     2041 tgcgggccaa cgctgcagcc gcactgcacg tgctgaggac agagcctccg gggaccttcc
     2101 tggtgcggaa atctaacact cgccagtgcc agactctatg tgtgcggctg ccagaagcta
     2161 gtggcccctc ttttgtctcc agccactatc tccaagagag tcctggcggc atctctttgg
     2221 agggctcaga gctcacattc ccggacctgg tccagctcat ctgtgcatac tgtcatacca
     2281 gggacattct tctcctccct ctccgactcc ccagagccat tcatcaggca gccacccaca
     2341 aagagctgga ggccatctcc catctgggca tggagttctg gagttcctct ctcaacacca
     2401 agaaccaaca gagaccttca gaagctccgc agataccccg gctgaaggct cgctcccctc
     2461 aagagctgga tcagggcact ggtgcagccc tctgcttctt caaccccctc ttccccgggg
     2521 atctgggccc caccaaacgt gagaaattca agaggagttt caaagtgcgt gtatccacag
     2581 agacctccag ccctctgtct ccacctgctg tgccgcctcc tccagtcccc gttctgccag
     2641 ggacatcttc cagccaaacg gaaagactgc cccctcgaca gctgctgcag agggagagct
     2701 cagtgggata ccgtgtgcca ggaagtgctt ctggtcctag tcttcctccc ctaccttcac
     2761 ttcaggaggt ggactgctgc tcccccagca gctcagagga ggagggttct tcaggaagcc
     2821 ctacaacctc cccacgccta agccgcccaa ggcaccggcg gcctctgctt cggtccatga
     2881 gttctgcttt ctgctctctg ctggcaccag agaggcaggt gggccgggca gctacaatgc
     2941 taatgcaaaa ccgatacaca gctgtgggtc agctagtgca ggacctattg acccaggttc
     3001 gtgttggtcc tgagtcccgg gagttgcagg gcatccgaca ggccctgagt cgggcacggg
     3061 ccatgctgag tgcagagctg ggccctgaga aactgctacc accagagcgg ctggaattgg
     3121 tcctggagaa atccctgcat cgctctgttc tcaagcctct tcgacccatt ctagctgccc
     3181 gcctgcggcg ccgactttcc acagacggtt cccttggccg cctagctgag ggcttccgcc
     3241 tggcccggac ccaggggcct ggagccttcg ggtcccacct gaacctttct tccccagtgg
     3301 agatagaacc agtgcgtcag aagctgctgc agctccttcg tgcctactcg cctagtgccc
     3361 agatcaagtg gctcctgcag gcctgcaagt tgctatacac agccctgaaa acccaggcag
     3421 gggagaatgc aggtgctgat gagttcctgc ctctcttgag ccttgtcttg gcccagtgcg
     3481 accttcctga cctcctgtta gaagctgagt atatgtcaga gctactggaa cccacattgc
     3541 tcactgggga gggcggctac tacctgacca gcctctcagc cagcctggcc ttgttgagtg
     3601 gcctaagcca ggcccacgca ctgccactca gccctgccca ggagctccaa cgttctctgg
     3661 ccctctggga acagcgccgc ctgccggcca cccacaactt ccagcacctc ctccgagtag
     3721 cctaccagga ccccagcaca ggttgcacct ccaagacctt ggctgtgccc ccggggtctt
     3781 cgattgccac cctgagtcaa ctctgtgcca ccaagttccg agtgacccaa ccggatgcat
     3841 ttggcctctt tctctacaag gaccagggct accatcgcct gccccctgaa gccctagtcc
     3901 acaggcttcc cacaactggt tacctcatct accgccgggc agagcgcccc gagacccaga
     3961 gggctgcaac agagaagacc aagacaggaa atgagaggcc agaggcagga gcatgggagg
     4021 aggagaaagg gggtctaaat ggtgaaggga aatctgagat agctgttgac caagaaggca
     4081 aggatcaggc cagaggaggt cacatgcagc tagaggaaca aaaggcagag ggatgcccgg
     4141 ctttagaaga gtaggcggcc gcgactctag agtcgacgga tctttttccc tctgccaaaa
     4201 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     4261 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     4321 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     4381 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     4441 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     4501 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     4561 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     4621 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4681 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4741 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4801 ttccagtcgg gaaacctgtc gtgccagcgg atacgcatct caattagtca gcaaccatag
     4861 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4921 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4981 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     5041 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5101 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5161 tgtctcgatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5221 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5281 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5341 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5401 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5581 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5701 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5761 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5821 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5881 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5941 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6001 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6061 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6121 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6181 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6241 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6301 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6361 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6421 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6481 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6541 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6601 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6661 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6721 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6781 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6841 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6901 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6961 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7021 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7081 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7141 ccccgaaaa
//
