LOCUS       Exported                7218 bp ds-DNA     circular SYN 15-AUG-2025
DEFINITION  25.JN_1-55.
ACCESSION   25.JN_1-55
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        400..676
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          677..1697
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.4"
                     /note="/other=intron"
     CDS             1695..1751
                     /label=HBB_LEPEU (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=94.7"
                     /note="/match_length=12.9"
                     /note="/other=CDS"
     CDS             1773..4220
                     /codon_start=1
                     /product="https://www.ncbi.nlm.nih.gov/nucleotide/NM_001106
                     120.1"
                     /label=Usp6nl_NM_001106120.1
                     /label=US6NL_MOUSE
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=91.1"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /translation="MNSDQDVALKLAQERAEIVAKYDRGREGAEIEPWEDADYLVYKVT
                     DRFGFLHEEELPYPNAAADRQKQLEIERTSKWLKMLKRWERYKNTEKFHRRIYKGIPLQ
                     LRGEVWALLLEIPKMKEETRDLYSKLKHRARGCSPDIRQIDLDVNRTFRDHIMFRDRYG
                     VKQQSLFHVLAAYSIYNTEVGYCQGMSQITALLLMYMNEEDAFWALVKLFSGPKHAMHG
                     FFVQGFPKLLRFQEHHEKILNKFLSKLKQHLDSQEIYTSFYTMKWFFQCFLDRTPFRLN
                     LRIWDIYIFEGERILTAMSYTILKLHRKHLMKLSMEELVEFLQETLAKDFFFEDDFVIE
                     QLQVSMTELKRAKLDLPEPGKEDEYPRKPLGQLPPEYTNHLSNGQRSVGRPSPKISSRR
                     EDGSPHKAHEHSPAHHSRNGTPERVGQSRRKSVDEGSKNLKQEADFQRKPSSGTQDNSK
                     HYNHATANQNSNALSNIRKEFVPKWRKPSEASAIERTAKYAIEGRSHSVFPALSVAVPS
                     SAETRLSNSRQKMKAFDGGEGNRGSNASQYDNVPGSESEPGASAEEGPERTHPHSPRKH
                     SEPSPSPSKVPNKFTFKVQPPSHVRYPSQPAGEDHRAAYPPSYSNPPVYHGNSPKHIPT
                     AHSGFASTQISPGLQVNPSRRPYGSSLSGDTSPEKSYGRLTPVLPSSRIEVLPIDIGAR
                     GYGSSGSPKSGKFILPPVDYLPENRKWSEFSYTYRPEVHGQSWTQDATRSHLSNLPKYT
                     AFPHIPLQDHGLPEVSVDSPVRYKTSPAVEEASPPGYPYAGPTPSAHHYRNREGLSVQE
                     SVLL"
     polyA_signal    4315..4370
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     protein_bind    4755..4771
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        4779..4809
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    4824..4845
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(4858..4897)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     promoter        4904..4926
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     rep_origin      4950..5085
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     polyA_signal    5105..5239
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(5241..5295)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     repeat_region   5307..5318
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=itrs"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.3"
                     /note="/other=repeat_region"
     rep_origin      5478..6066
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(6237..7097)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        7098..7202
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
      481 aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccag gcggggcggg
      541 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      601 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      661 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
      721 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
      781 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
      841 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
      901 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
      961 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1021 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     1081 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     1141 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     1201 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     1261 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     1321 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     1381 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     1441 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     1501 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     1561 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     1621 cggcttctgg cgtgtgaccg gcggctctag ctagagcctc tgctaaccat gttcatgcct
     1681 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     1741 gcaaagaatt cgctagcgct accggtgcca ccatgaattc ggaccaggat gtagcgctca
     1801 aactcgctca ggaacgagct gaaatagttg ctaaatatga cagaggacgg gaaggtgcag
     1861 agatcgagcc ctgggaagat gctgattatc ttgtttacaa agtcaccgat agatttggct
     1921 ttttacatga ggaggagctc ccgtatccta acgcagccgc ggaccggcaa aagcaactgg
     1981 aaatcgaaag gacttccaag tggctgaaaa tgctgaagag atgggagaga tacaagaaca
     2041 ctgagaagtt ccatcgacga atttacaaag gaattccact ccagctccga ggtgaagtct
     2101 gggcccttct tcttgagatc cctaaaatga aagaagaaac aagagacctc tatagtaaat
     2161 taaagcacag agcacggggt tgttcgcctg atatcagaca aatagacctt gacgtgaacc
     2221 gcacattcag ggaccatatc atgtttagag acagatatgg tgtcaagcag caatccttgt
     2281 tccacgttct tgctgcctac tccatctaca atacggaggt cgggtactgt caagggatga
     2341 gtcagatcac agctctgctc ctgatgtaca tgaatgagga ggacgccttc tgggccctgg
     2401 tcaagctctt ctcaggcccc aagcacgcca tgcatggttt ttttgtccaa ggtttcccta
     2461 aactcttgag atttcaagaa catcatgaga aaattctgaa caaatttttg tccaaactaa
     2521 agcagcactt ggattctcaa gaaatctata caagttttta cacaatgaag tggttttttc
     2581 agtgtttcct tgaccgaact cccttcaggc tgaacctccg gatctgggac atctacatct
     2641 ttgagggaga gcgaattctc actgctatgt cttatacaat cttgaagcta cacagaaagc
     2701 atctaatgaa gttgtctatg gaagaacttg tagaatttct tcaggagact ctcgccaagg
     2761 actttttctt cgaggatgat ttcgtaatag agcaactgca ggtctctatg acagagttaa
     2821 agcgggccaa gttagatctc ccagaacctg gtaaggagga tgaataccca aggaaacccc
     2881 tggggcagct cccacctgag tataccaatc acttgagcaa tggacaaaga agtgttggca
     2941 ggcccagccc caaaataagc agcaggagag aagatgggtc gcctcacaag gcacatgagc
     3001 actctcctgc ccatcacagc agaaatggca ccccagaaag agtcgggcag tcaagacgca
     3061 agtctgtgga tgagggtagt aaaaatctga agcaggaggc agattttcag agaaaacctt
     3121 catcgggcac acaggacaac tccaagcatt ataatcatgc aactgctaac caaaatagca
     3181 atgcactttc caacatcagg aaggagttcg tgcccaaatg gaggaagcca tcggaggcct
     3241 cagctatcga aagaactgct aaatacgcca ttgaaggcag aagtcactca gtgtttcccg
     3301 ctctctctgt cgcagtccca agttctgctg agacccgatt gtcaaactca aggcaaaaga
     3361 tgaaggcttt tgatggtggt gaggggaacc ggggttctaa tgcttcccag tatgacaacg
     3421 tacctgggag tgagagcgag cctggagcgt ctgcagagga gggaccggag cggactcacc
     3481 ctcacagtcc aaggaagcac tctgagccaa gtcccagtcc atcaaaagta cccaacaagt
     3541 ttacctttaa agtgcagcct cccagccatg tacgataccc atcccagcca gcaggggagg
     3601 atcacagagc tgcatatcca ccctcctaca gcaacccgcc tgtctaccat ggaaattctc
     3661 caaaacacat ccctacggcc cacagtggct ttgcgtccac acagatcagc ccggggcttc
     3721 aagtgaaccc ttccaggaga ccttacggtt cctctctttc aggtgacact tctccagaga
     3781 agtcctacgg ccgcctaact cctgttcttc cctctagtcg aattgaagtc cttcccatag
     3841 acattggtgc caggggatac ggcagttcag ggtcaccaaa gagcggaaag ttcatccttc
     3901 ccccggtgga ctatttgcct gagaacagaa aatggtcaga atttagttac acatacagac
     3961 ctgaggtgca tggtcagtca tggactcagg atgctacccg tagccactta agcaatttac
     4021 caaaatacac agcctttccg cacatacctc ttcaggacca cggccttcca gaagtgtctg
     4081 tagacagtcc agtgaggtat aagacttcac cagctgtgga agaggccagt cctcctgggt
     4141 atccatacgc agggcccaca ccttcagcac atcactacag aaatcgagag ggcctgtctg
     4201 ttcaggaatc agtgttgcta tgagcggccg cgactctaga gtcgacggat ctttttccct
     4261 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     4321 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     4381 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     4441 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     4501 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     4561 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     4621 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     4681 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     4741 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4801 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4861 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
     4921 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4981 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5041 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5101 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5161 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5221 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5281 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5341 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5401 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5461 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5521 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5581 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5641 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5701 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5761 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5821 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5881 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5941 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6001 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6061 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6121 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6181 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6241 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6301 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6361 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6421 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6481 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6541 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6601 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6661 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6721 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6781 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6841 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6901 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6961 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7021 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7081 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7141 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7201 cgcacatttc cccgaaaa
//
