LOCUS       Exported                7104 bp ds-DNA     circular SYN 15-AUG-2025
DEFINITION  26.JN_1-56.
ACCESSION   26.JN_1-56
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        400..678
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.4"
                     /note="/other=promoter"
     intron          679..1699
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.4"
                     /note="/other=intron"
     CDS             1697..1753
                     /label=HBB_LEPEU (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=94.7"
                     /note="/match_length=12.9"
                     /note="/other=CDS"
     CDS             1802..3970
                     /codon_start=1
                     /product="https://www.ncbi.nlm.nih.gov/nucleotide/BC062060.
                     1"
                     /label=Sgsm3[1-723]_BC062060.1
                     /label=SGSM3_RAT
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=96.5"
                     /note="/other=CDS"
                     /translation="MSGNHTPSASGPFSALTPSIWPQEILAKSSQKEDSSEPEICYDEF
                     GFRVDKEGSEPGCSQMAGTPLVEDPPQRLRWQAHLEFTHNHDVGDLTWDKIAVSLPRSE
                     KLRSLVLAGIPHGMRPQLWMRLSGALQKKKNSELSYREIVKNSSNDETIAAKQIEKDLL
                     RTMPSNACFANVNSIGVPRLRRVLRALAWLYPEIGYCQGTGMVAACLLLFLEEEDAFWM
                     MCAIIEDLLPASYFSTTLLGVQTDQRVLRHLIVQYLPRLDKLLQEHDIELSLITLHWFL
                     TAFASVVHIRLLLRIWDLFFYEGSLVLFQTTLGMLRLKEEELIQSENSASIFNTLSDIP
                     AQMDDAELLLGEAMQLAGSLTDVAVETQRRKHLAYLIADQGQTLGTSTTTSLSQVVRRR
                     TQRRKSGITSLLFGEDDLEALKAKNIKQTELVADLREAILRVARHFQCTDPKNCSVELT
                     PDYSMESHQRDHENYVACLRSHRRRAKALLDFERHDDDELGFRKNDIITIISQKDEHCW
                     VGELNGLRGWFPAKFVEVLDERSKEYSIAGDDSVTEGVTDLVRGTLCPALKALFEHGLK
                     KPSLLGGACHPWLFIEEAAGREVERDFDSVYSRLVLCKTYRLDEDGKVLTPEELLYRAV
                     QSVNVTHDAAHAQMDVKLRSLICVGLNEQVLHLWLEVLCSSLPTVEKWYQPWSFLRSPG
                     WVQIKCELRVLCCFAFSLSQDWELPAKRE"
     CDS             4026..4106
                     /label=SGSM3_RAT (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.3"
                     /note="/match_length=3.6"
                     /note="/other=CDS"
     polyA_signal    4201..4256
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     primer_bind     complement(4617..4633)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4641..4657
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        4665..4695
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    4710..4731
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(4744..4783)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     promoter        4790..4985
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4790..4812
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     rep_origin      4836..4971
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     polyA_signal    4991..5125
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(5127..5181)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     repeat_region   5193..5204
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=itrs"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.3"
                     /note="/other=repeat_region"
     rep_origin      5364..5952
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(6123..6983)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        6984..7088
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc ccccccctcc ccacccccaa ttttgtattt atttattttt
      481 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg
      541 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
      601 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
      661 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
      721 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
      781 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
      841 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
      901 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
      961 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     1021 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     1081 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     1141 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     1201 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     1261 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
     1321 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
     1381 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
     1441 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
     1501 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
     1561 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
     1621 ttcggcttct ggcgtgtgac cggcggctct agctagagcc tctgctaacc atgttcatgc
     1681 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     1741 tggcaaagaa ttcgccacca tgcctagatc tatgaagagc tgccatcgtc cacagcacat
     1801 gatgtcagga aaccacacgc cttctgctag tggccctttc tctgccctga ctccaagcat
     1861 atggccccag gagatcctgg caaaatcctc acagaaggaa gactcctcag agccagagat
     1921 ctgctatgat gagtttggtt tccgagtgga caaggaaggt tctgagcctg gatgcagcca
     1981 gatggcaggc acacccctgg tagaggatcc ccctcagagg ctacgttggc aggctcacct
     2041 ggagtttacc cacaaccatg atgttgggga tctcacctgg gacaagattg cagtctccct
     2101 cccccgctct gagaaactcc gttccctagt gctggctggc atcccgcatg gcatgaggcc
     2161 acagttgtgg atgcggctct ctggggcact gcagaagaag aagaactctg agttgtccta
     2221 ccgtgagatt gtaaagaaca gctccaatga tgagaccatc gctgccaaac agatcgaaaa
     2281 ggacctgctc cgtaccatgc ccagcaacgc ctgctttgcc aatgtgaaca gcatcggagt
     2341 gccccgcctg cgcagagtcc ttcgagcact ggcttggctc tatccagaga ttggctactg
     2401 ccagggcaca ggcatggtgg ctgcctgtct cctgctgttc ctggaggagg aggacgcctt
     2461 ctggatgatg tgtgccatta ttgaggacct gctgcctgcc tcctacttca gcaccaccct
     2521 gctaggtgtc cagactgacc agcgggtcct gcgccacctg attgtccagt acctgcctcg
     2581 cttagacaag ctgttgcagg agcatgacat tgagctgtct ctgatcacgc tgcactggtt
     2641 cctcacggcc ttcgccagcg tggtgcacat cagactgctg ctgcgcatct gggacctgtt
     2701 cttctacgag ggctccctgg tgttgttcca gactacgctg ggcatgctgc gccttaagga
     2761 ggaagagctg atacagtcgg agaactcagc gtccatcttc aacacgctgt cggacatccc
     2821 tgcccagatg gatgatgcag agctgctgct gggggaggcc atgcagctgg ctggctccct
     2881 cactgatgtg gctgtggaga cccagcgccg caagcatctg gcctacctca ttgcagacca
     2941 aggccagacc ctggggacga gcaccaccac cagcctctct caggttgtac ggcgaaggac
     3001 ccagcggaga aagtctggca tcacctccct gctctttgga gaggatgacc tagaagctct
     3061 caaggcaaag aacatcaaac aaacagagct ggtagctgac cttcgtgaag ccattttacg
     3121 tgtggcacgc cacttccagt gcacagaccc caagaactgt agtgtggagc tgacgccaga
     3181 ctacagcatg gagagccacc agcgggacca tgagaactat gtggcatgct tacgcagcca
     3241 ccggcgccgg gccaaggccc tgctggactt tgagcgtcat gacgatgacg agctaggctt
     3301 ccgcaaaaat gacatcatca cgatcatttc tcagaaggat gagcactgct gggtgggtga
     3361 gctgaacggc ctgagaggct ggtttccagc caagtttgtg gaggtcctgg atgagcgaag
     3421 taaagagtac tccattgcgg gggatgactc tgtgactgaa ggagtaacag acctcgtgcg
     3481 agggaccctc tgcccagccc tcaaggccct atttgaacat ggattgaaga agccatcctt
     3541 gcttgggggt gcttgccacc cctggctgtt tattgaggag gcagcaggcc gggaagtcga
     3601 gagagacttt gattctgtgt actcccgcct ggtgctctgt aagacctaca ggttggatga
     3661 agacggcaaa gtcctgaccc ctgaagagct gctgtaccgg gccgtgcagt ctgtgaatgt
     3721 gacccatgat gcagcacacg cacagatgga tgtcaaactc cgttcactta tctgtgtggg
     3781 gctcaatgag caggtcctac acctgtggct agaggtactc tgctccagcc tgcccaccgt
     3841 ggagaagtgg taccagccct ggtccttcct gcgcagccct ggctgggtcc agatcaagtg
     3901 tgagctccgt gtcctctgct gctttgcctt cagcctctcc caggactggg agctccccgc
     3961 taagagagag aagtgacctg tggttccagg gtggttcctg cccatgacta gggctgttct
     4021 gtgcgcagga ggagaagcag ccactgaagg agggtgttca ggacatgctg gtgaagcacc
     4081 atctcttcag ctgggacata gatggatgag cggccgcgac tctagagtcg acggatcttt
     4141 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     4201 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     4261 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     4321 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     4381 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     4441 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     4501 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     4561 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca
     4621 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4681 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4741 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt
     4801 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4861 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4921 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4981 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5041 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5101 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga
     5161 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5761 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7081 gttccgcgca catttccccg aaaa
//
