LOCUS       Exported                5668 bp ds-DNA     circular SYN 16-AUG-2025
DEFINITION  32.JN_4-48.
ACCESSION   32.JN_4-48
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        33..336
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        337..540
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     regulatory      579..588
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             585..1292
                     /codon_start=1
                     /label=mCherry
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             1314..1586
                     /codon_start=1
                     /product="https://www.ncbi.nlm.nih.gov/nucleotide/NM_001427
                     400.1"
                     /label=Eea1[36-126]_NM_001427400.1
                     /label=EEA1_MOUSE (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=93.8"
                     /note="/match_length=6.9"
                     /note="/other=CDS"
                     /translation="SSSEGFICPQCMKSLGSADELFKHYQAVHDAGNDSGHGGEASLAL
                     TRDDIALLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKS"
     CDS             1596..2303
                     /codon_start=1
                     /label=mCherry
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    2428..2549
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      complement(2556..3011)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(2583..3011)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        3038..3142
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        3144..3501
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     enhancer        3144..3335
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      3352..3487
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3536..4330
                     /label=NeoR/KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             4351..4503
                     /label=BLE_KLEPN (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=40.5"
                     /note="/other=CDS"
     polyA_signal    4562..4609
                     /label=HSV TK poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     polyA_signal    complement(4700..4716)
                     /label=bGH poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.2"
                     /note="/other=polyA_signal"
     rep_origin      4938..5526
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
       61 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      121 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      181 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      241 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      301 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      361 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      421 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      481 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      541 ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
      601 aggaggacaa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
      661 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      721 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
      781 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      841 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      901 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      961 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1021 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     1081 tcaagcagag gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct
     1141 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagctggaca
     1201 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc
     1261 actccaccgg cggcatggac gagctgtaca agtccggact cagatctcga ggaagttctt
     1321 cagagggttt tatatgtccc cagtgtatga aatctcttgg ctcagctgat gaacttttca
     1381 aacactatca ggcagttcat gatgccggta atgactcggg gcatggtgga gaggccagtc
     1441 ttgctttgac acgagatgac atagcactac tcaggcaaga ggtccaagac ctgcaggcct
     1501 cacttaagga agaaaaatgg tactcagaag aattaaagaa agaattagaa aaatatcaag
     1561 ggctacagca gcaagaggct aaatctggat ccggaatggt gagcaagggc gaggaggaca
     1621 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     1681 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     1741 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc
     1801 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     1861 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     1921 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     1981 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2041 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     2101 ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc tacaaggcca
     2161 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagctggac atcacctccc
     2221 acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg
     2281 gcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc
     2341 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2401 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     2461 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2521 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
     2581 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2641 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2701 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     2761 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     2821 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     2881 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     2941 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     3001 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3061 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3121 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     3181 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3241 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3301 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3361 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3421 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3481 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     3541 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3601 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3661 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     3721 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3781 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3841 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3901 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3961 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4021 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     4081 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4141 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4201 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4261 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4321 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     4381 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4441 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4501 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     4561 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     4621 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     4681 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     4741 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     4801 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4861 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4921 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4981 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5041 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5101 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5161 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5221 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5281 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5341 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5401 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5461 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5521 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5581 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     5641 tagttattaa tagtaatcaa ttacgggg
//
