LOCUS       Exported                6887 bp ds-DNA     circular SYN 15-AUG-2025
DEFINITION  02.JW_1-G9.
ACCESSION   02.JW_1-G9
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        400..678
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.4"
                     /note="/other=promoter"
     intron          679..1699
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.4"
                     /note="/other=intron"
     CDS             1697..1753
                     /label=HBB_LEPEU (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=94.7"
                     /note="/match_length=12.9"
                     /note="/other=CDS"
     regulatory      1769..1778
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1781..1807
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1811..1837
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1841..1867
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1880..3889
                     /codon_start=1
                     /product="https://www.ncbi.nlm.nih.gov/nucleotide/BC053395.
                     1?report=genbank&log$=nucltop&blast_rank=1&RID=9YAZ66W5015"
                     /label=mTBC1D15_BC053395.1
                     /label=TBC15_MOUSE
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
                     /translation="AAAGVVSGKIIYEQEGVYIHSSCGKANDQDSLISGILRVLEKDAE
                     VIVDWRPLDDALDSSSILCAGKDSSSVVEWTQAPKERAHRGSDQQSSYEAEWDMVTTVS
                     FKKKPHTNGDAPGHRNGKSKWSFLFSLADLKSVKQSKEGMGWSYLVFCLKDDVMLPALH
                     FHQGDSKLLIESLEKYVVLCESPQDSRTLLVNCQNKSLSQSFENLLDEPAYGLTQKIKK
                     DPYTATMVGFSKVTNYIFDSLRGSDPSTHQRPPSEMADFLSDAIPGLKINQQEEPGFEV
                     ITRIDLGERPVVQRREPVSLEEWNKSLDPEGRLVAVESMKQKIFRGGLSHSLRKQAWKF
                     LLGYFPWDSTKEERTQLQKQKTDEYFRMKLQWKSVSEAQEKRNSRLRDYRSLIEKDVNR
                     TDRTNKFYEGQDNPGLILLHDILMTYCMYDFDLGYVQGMSDLLSPLLYVMENEVDAFWC
                     FASYMDQMHQNFEEQMQGMKTQLIQLSTLLRLLDSGFCSYLESQDSGYLYFCFRWLLIR
                     FKREFSFLDILRLWEVMWTELPCKNFHLLLCCAILESEKQQIMAKHYGFNEILKHINEL
                     SMKIDVEDILCKAEAISLQMAQCKELPQAVCEILGLQDNEITTPDSDTDENVGSPCPVS
                     AFPSSTLPILAASEAKDDSPTQTLASPNACRLTPA"
     polyA_signal    3984..4039
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     primer_bind     complement(4400..4416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4424..4440
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        4448..4478
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    4493..4514
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(4527..4566)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     promoter        4573..4768
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4573..4595
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     rep_origin      4619..4754
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     polyA_signal    4774..4908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4774..4895
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(4910..4964)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     repeat_region   4976..4987
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=itrs"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.3"
                     /note="/other=repeat_region"
     rep_origin      5147..5735
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(5906..6766)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        6767..6871
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc ccccccctcc ccacccccaa ttttgtattt atttattttt
      481 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg
      541 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
      601 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
      661 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
      721 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
      781 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
      841 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
      901 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
      961 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     1021 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     1081 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     1141 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     1201 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     1261 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
     1321 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
     1381 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
     1441 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
     1501 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
     1561 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
     1621 ttcggcttct ggcgtgtgac cggcggctct agctagagcc tctgctaacc atgttcatgc
     1681 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     1741 tggcaaagaa ttcgctagcg ctaccggtgc caccatggca tacccttatg acgtaccaga
     1801 ttacgcggga tatccctacg atgtccccga ttacgcgcgt tatccatatg acgtcccgga
     1861 ctatgccgaa ttcctcgagg cggcggcggg tgttgtgagc gggaagatta tatatgaaca
     1921 agaaggagta tatattcact catcttgcgg aaaggcaaat gaccaagaca gcttgatttc
     1981 aggaatattg cgtgttttag aaaaggatgc tgaagtaata gtggactgga gaccattgga
     2041 tgatgcatta gattcttcca gtattctgtg tgctggaaag gactccagct cagtcgtaga
     2101 gtggactcag gctccaaagg aaagagctca tcgggggtcg gaccagcaga gcagctatga
     2161 agcggagtgg gacatggtta ctacagtgtc ctttaagaag aaaccacaca ccaatggaga
     2221 tgctccaggc cataggaacg ggaaaagcaa atggtcgttc ctgttcagcc tggcagacct
     2281 gaagtcagtc aagcagagca aggagggcat gggctggtcg tacctggtct tctgcctgaa
     2341 ggatgacgtc atgctgcctg ccctgcactt ccatcaagga gacagcaaac tgctgatcga
     2401 gtctcttgag aaatacgtgg tattgtgtga gtcaccacaa gatagtagaa cacttctagt
     2461 caattgtcag aataagagtc tctcacagtc ttttgaaaat cttcttgatg aaccagcata
     2521 tggcttaaca caaaaaatta aaaaggatcc ttatacagca accatggtag gattttccaa
     2581 agttacaaac tacatttttg acagtttgcg aggcagcgat ccctctacac atcaacgacc
     2641 accttcagaa atggcagatt ttcttagtga cgctatccca ggactcaaga taaaccagca
     2701 agaagagcca ggatttgaag tcatcactag aattgatttg ggagagcgac ctgttgtgca
     2761 aaggcgggag ccagtgtccc tggaagaatg gaacaagagc cttgaccctg aaggaagact
     2821 cgtagccgtg gagagcatga agcaaaagat attcagaggg ggtcttagtc attcattaag
     2881 aaagcaagca tggaaatttc tcctgggtta ttttccttgg gatagtacca aagaggaaag
     2941 aactcagtta caaaaacaaa aaactgatga gtacttcagg atgaaactgc agtggaagtc
     3001 tgtcagtgag gctcaggaga agagaaactc acgcctgaga gactacagaa gtctgatcga
     3061 gaaagatgtc aacagaacag atcgaacgaa caagttttat gaaggccaag ataacccagg
     3121 gctgattctg cttcacgaca ttttgatgac ctactgtatg tatgactttg atttaggata
     3181 tgtacaagga atgagcgact tactttcccc acttttgtat gtgatggaga atgaagtgga
     3241 tgccttttgg tgctttgcct cttacatgga ccagatgcat caaaattttg aagaacaaat
     3301 gcagggcatg aagacgcagt taatccagtt gagtacctta ctcaggttac tggacagtgg
     3361 attttgtagc tatttagaat cccaggactc tggatatcta tatttttgct tcagatggct
     3421 tttaatcaga tttaaaaggg aatttagttt tctagatata cttcgattat gggaggtaat
     3481 gtggactgaa ctgccctgta agaattttca tcttcttctg tgttgtgcca tcctggaatc
     3541 agaaaagcaa cagataatgg caaagcacta tggcttcaat gaaatcctta agcacatcaa
     3601 tgagttgtcc atgaagattg atgtggaaga tatactctgt aaagcagaag caatttccct
     3661 acagatggca caatgcaagg aattgccaca agcagtttgt gagatcctgg gacttcagga
     3721 caatgaaatt acaacgccag attccgacac tgatgaaaat gttggcagcc cttgtcctgt
     3781 atctgcgttt ccaagtagca ccttgcccat ccttgctgcc agtgaagcca aagatgacag
     3841 tccaacacag acattagcat ccccgaatgc ctgcagatta acacctgcat gagcggccgc
     3901 gactctagag tcgacggatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     3961 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     4021 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     4081 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     4141 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     4201 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     4261 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     4321 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     4381 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4441 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4501 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4561 gccagcggat acgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4621 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4681 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4741 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
     4801 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4861 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctcgatccg ctgcattaat
     4921 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4981 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5041 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5101 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5161 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5221 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5281 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5341 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5401 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5461 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5521 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5581 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5641 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5701 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5761 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5821 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5881 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5941 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6001 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6061 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6121 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6181 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6241 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6301 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6361 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6421 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6481 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6541 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6601 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6661 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6721 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6781 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6841 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaa
//
