LOCUS       Exported                7152 bp ds-DNA     circular SYN 18-AUG-2025
DEFINITION  31.JN_2-55.
ACCESSION   31.JN_2-55
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        400..676
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          677..1697
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.4"
                     /note="/other=intron"
     CDS             1695..1751
                     /codon_start=1
                     /label=HBB_LEPEU (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=94.7"
                     /note="/match_length=12.9"
                     /note="/other=CDS"
                     /translation="QLLGNVLVIVLSHHFGKEF"
     regulatory      1767..1776
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1779..1805
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1809..1835
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1839..1865
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1881..4154
                     /codon_start=1
                     /product="https://www.ncbi.nlm.nih.gov/nucleotide/NM_001427
                     595.1"
                     /label=TBC1D30_NM_001427595.1
                     /label=TBC30_MOUSE
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=94.6"
                     /note="/match_length=99.0"
                     /note="/other=CDS"
                     /translation="MRQDKLTSSLRRGGRCLKRQGGGGVGTILSNVLKKRSCISRTAPR
                     LLCTLEPGVDTKLKFTLEPSLGQNGFQQWYDALKAVARLSTGIPKEWRRKVWLTLADHY
                     LHSIAIDWDKTMRFTFNERSNPDDDSMGIQIVKDLHRTGCSSYCGQEAEQDRVVLKRVL
                     LAYARWNKNVGYCQGFNILAALILEVMEGNEGDALKIMIYLIDKVLPESYFVNNLRALS
                     VDMAVFRDLLRLKLPELSQHLDTLQRTSNKESGGGYEPPLTNVFTMQWFLTLFATCLPN
                     HTVLKIWDSVFFEGSEIILRVSLAIWAKLGEQIECCETADEFYGTMGRLTQEMLEHDLL
                     QSHELMQTVYSMAPFPFPQLAELREKYTYNITPFPATIKPTSVSGRHSKSRDSDDENGP
                     DDEDAVASAVGCLGPLSGLLAPELQKYQKQIKEGTEEQTLRSNNIAELSPGAINSCRSE
                     YHAAFNSMMTERMTTDINALKRQYSRIKKKQQQQLHQVYIRADKGPVTSILPSQANSSP
                     VINHLLLGKKMKITNRAAKNAVIHMPGHAGGKISPVPYEDIKTKLNSPWRTHIRVHKKN
                     MPRTKSHLGCGDTVGLIEEQNEGCKTSSLGAAEEFPSSCAVTAQSEGSSGDSVTPRTIE
                     GQSPEPVFGDADVDVTAVQVKLEALELNQRDTTAETESKGQRHAPELVDAPGENQTVSK
                     SLPGSNSKPPIFSPFPSVKPLRKSATARNLGLYGPTERTPTMHFPQMSRGFSKSGMGSS
                     STKKR"
     polyA_signal    4249..4304
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     primer_bind     complement(4665..4681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4689..4705
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        4713..4743
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    4758..4779
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(4792..4831)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     promoter        4838..5033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4838..4860
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     rep_origin      4884..5019
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     polyA_signal    5039..5173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5039..5160
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(5175..5229)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     repeat_region   5241..5252
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=itrs"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.3"
                     /note="/other=repeat_region"
     rep_origin      5412..6000
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(6171..7031)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        7032..7136
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
      481 aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccag gcggggcggg
      541 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      601 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      661 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
      721 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
      781 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
      841 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
      901 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
      961 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1021 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     1081 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     1141 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     1201 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     1261 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     1321 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     1381 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     1441 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     1501 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     1561 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     1621 cggcttctgg cgtgtgaccg gcggctctag ctagagcctc tgctaaccat gttcatgcct
     1681 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     1741 gcaaagaatt cgctagcgct accggtgcca ccatggcata cccttatgac gtaccagatt
     1801 acgcgggata tccctacgat gtccccgatt acgcgcgtta tccatatgac gtcccggact
     1861 atgccgaatt cctcgagctc atgcggcagg acaaactgac cagctccctg cgtcgcggcg
     1921 ggcgatgcct gaagcgccag ggcggcggtg gcgtgggcac catcctgagc aatgtgctca
     1981 agaagcgcag ctgcatctcg cggaccgcgc cccggttgct ctgcaccttg gagccgggag
     2041 ttgatacaaa attgaagttc acacttgaac catctttggg tcaaaatggt tttcagcagt
     2101 ggtatgatgc tctcaaggca gttgccagac tgtcaacggg gatcccgaag gaatggagga
     2161 ggaaagtttg gttgaccttg gcggatcact atttgcatag tatagccatc gactgggaca
     2221 aaaccatgcg cttcactttc aacgagagga gcaaccccga tgacgactca atggggattc
     2281 agatcgtcaa ggatctgcat cgcacgggct gtagttccta ctgtggccag gaggcggagc
     2341 aagacagggt ggtactaaag cgggtgctat tggcttacgc ccggtggaac aagaatgtcg
     2401 ggtactgtca aggcttcaac atcctggctg ccctcattct ggaagtgatg gaaggcaacg
     2461 agggggacgc gctgaagatt atgatttacc tgattgataa ggtgctccct gaaagctact
     2521 ttgtcaataa tctccgggca ctgtctgtgg atatggctgt cttcagagac ctcctgcgac
     2581 tgaagctccc tgagctgtct cagcacctcg acactcttca gagaacttca aacaaagaga
     2641 gcggaggcgg atatgagccc ccgctcacca atgtcttcac gatgcagtgg ttcctcacgc
     2701 tctttgccac gtgcctccct aaccacacgg ttctgaagat ctgggactca gtattctttg
     2761 aaggttcaga gatcatcctg agggtgtcgc tggctatctg ggcaaaactg ggagagcaga
     2821 tagaatgttg tgaaacagca gacgagttct acggtaccat ggggcgcctt acccaggaga
     2881 tgctggagca tgatctcttg cagagccatg agctcatgca gacagtgtat tcgatggccc
     2941 cattcccttt cccgcagttg gcggagttga gggagaagta tacctacaac attacaccat
     3001 ttccagccac aatcaagccc acttcagttt ctgggcgaca cagtaagtcc agagatagtg
     3061 acgatgagaa cggcccggac gatgaggatg ctgtcgctag tgctgtgggg tgccttggac
     3121 cgctcagtgg gctcctggct cctgaactgc agaagtacca aaagcagatt aaagaaggga
     3181 cagaggaaca gaccctgagg tcgaacaaca tcgcagagct gagcccagga gccatcaatt
     3241 cgtgtcgaag tgagtaccac gcagcgttca acagcatgat gacagagcgg atgaccacgg
     3301 acatcaacgc cctgaagagg cagtactcgc gcattaagaa gaagcagcag cagcagctcc
     3361 accaggtgta cataagagca gacaaagggc cagtgaccag catcctccca tctcaggcaa
     3421 atagttctcc ggtgatcaac catcttcttc tagggaaaaa gatgaaaatt acaaacagag
     3481 cagccaagaa tgcagtcatc cacatgcctg gccacgcggg aggaaaaata tctcctgtcc
     3541 cttacgaaga cattaagaca aagctcaact ctccgtggcg gactcacatc cgagtccaca
     3601 aaaagaacat gccaaggacc aagagccatc tgggctgtgg agacacagta gggctaatag
     3661 aagaacagaa tgaaggctgt aagaccagca gcttgggggc agctgaggag tttccctcca
     3721 gttgtgcagt aacagctcag agtgagggca gcagcggtga cagcgtcact ccgaggacaa
     3781 tcgaagggca gtctccagag cccgtatttg gggatgctga cgttgatgtg actgcagttc
     3841 aggtgaaatt agaagccttg gaacttaacc aaagggatac cacggcagaa actgagtcca
     3901 aggggcagcg gcatgcccca gagttagtcg atgcccctgg agaaaaccag actgtcagca
     3961 aatctctccc gggaagcaac tcaaaacccc ccatcttcag tcccttcccc agtgtcaagc
     4021 cgctgcgaaa gtcagccaca gccaggaatt tgggattata tggccctacc gaaagaaccc
     4081 caaccatgca cttccctcag atgagcaggg gcttcagcaa gtcaggcatg gggagcagca
     4141 gcaccaagaa gcgatgagcg gccgcgactc tagagtcgac ggatcttttt ccctctgcca
     4201 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     4261 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     4321 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     4381 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     4441 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     4501 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     4561 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     4621 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     4681 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4741 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4801 gctttccagt cgggaaacct gtcgtgccag cggatacgca tctcaattag tcagcaacca
     4861 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4921 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4981 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
     5041 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5101 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5161 tcatgtctcg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5221 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5281 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5341 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5581 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5821 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7141 tttccccgaa aa
//
