LOCUS       Exported                7816 bp ds-DNA     circular SYN 15-AUG-2025
DEFINITION  22.JW_4-C3.
ACCESSION   22.JW_4-C3
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        400..677
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          678..1698
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.4"
                     /note="/other=intron"
     CDS             1696..1752
                     /label=HBB_LEPEU (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=94.7"
                     /note="/match_length=12.9"
                     /note="/other=CDS"
     regulatory      1768..1777
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1780..1806
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1810..1836
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1840..1866
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1879..4818
                     /codon_start=1
                     /product="https://www.ncbi.nlm.nih.gov/nucleotide/BC046297.
                     1"
                     /label=Rab3gap1_BC046297.1
                     /label=RB3GP_MOUSE
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
                     /translation="AADSEPESEVFEITDFTTASEWERFISKVEEVLNDWKLIGPSLGK
                     PLEKGIFTSGTWEERSDEISFADFRFSVTHHYLVQESPDKEGKDEELEDAIPQSMQDLL
                     CMNNDFPPRAHCLVRWYGLREFVVIAPAAHSDAVLSESKCNLLLSSISIALGNTGCQVP
                     LFVQIHHKWRRMYMGECQGPGVRTDFEMVHLRKVPSQYTHLSGLLDIFKSKIGCPLTPL
                     PPVSIAIRLTYVLQDWQQYFWPQQPPDIDALVGGEVGGLEFGKLPFGACEDPISELHLA
                     TTWPHLTEGIIVDNDVYSDLDPVQAPHWSVRVRKADNPQCLLGDFVTEFLKICRRKEST
                     DEILGRSTFEEEGREVADITHALSKLTEPAPVPIHKLSVSNMVHTAKKKIRKHRGEESP
                     LNSDVLNTILLFLFPDAVSEKPLDGTTSIDNSIPAPEAGDYTLYNQFKSAPSDSLTYKL
                     ALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGLASGSPDLRCCLLHQKLQM
                     LNCCIERKKARDEGKKTSLSDSTTSAYPGDAGKTGGQLGLDHLRDTEKEKGEVGKSWDS
                     WSDSEEEFFECLSDTEDLKGNGQESGKKGGPKEMANLKPEGRLHQHGKLTLLHNGEPLY
                     IPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCFL
                     EDFVRWYSPRDYIEEEVTDEKGNVVLKGELSARMKIPSNMWVEAWETAKPVPARRQRRL
                     FDDTREAEKVLHYLAMQKPADLARHLLPCVIHAAVLKVKEEESLENIPSVKKIIKQIIA
                     HSSKVLHFPNPEDKKLEEIILQITTVEAIIARARSLKAKFGTEKCEHEEEKEGLERFVS
                     CLLEQPEVSVTGAGRGHAGRIIHKLFVNAQRAAAVALPEEELKKSGCPEERRQTLVSDF
                     PPPAGRELILRATVPRPAPYSKALPQRMYSVLTKEDFRLAGAFSSDTSFF"
     polyA_signal    4913..4968
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     primer_bind     complement(5329..5345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5353..5369
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        5377..5407
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    5422..5443
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(5456..5495)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     promoter        5502..5697
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5502..5524
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     rep_origin      5548..5683
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     polyA_signal    5703..5837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5703..5824
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(5839..5893)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     repeat_region   5905..5916
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=itrs"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.3"
                     /note="/other=repeat_region"
     rep_origin      6076..6664
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(6835..7695)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        7696..7800
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
      481 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg
      541 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
      601 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
      661 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
      721 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
      781 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
      841 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
      901 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
      961 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     1021 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     1081 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     1141 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     1201 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     1261 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     1321 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     1381 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     1441 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     1501 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     1561 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     1621 tcggcttctg gcgtgtgacc ggcggctcta gctagagcct ctgctaacca tgttcatgcc
     1681 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     1741 ggcaaagaat tcgctagcgc taccggtgcc accatggcat acccttatga cgtaccagat
     1801 tacgcgggat atccctacga tgtccccgat tacgcgcgtt atccatatga cgtcccggac
     1861 tatgccgaat tcctcgaggc ggcggacagt gagcccgagt ccgaagtgtt tgagatcaca
     1921 gacttcacca cagcctcgga gtgggagagg tttatttcca aagttgaaga agtcttgaat
     1981 gactggaaac tgattggacc ctctctggga aagccactgg aaaagggtat atttacttct
     2041 ggcacatggg aagagcgatc agatgaaatt tcctttgctg acttcaggtt ttcagtcaca
     2101 catcactatc ttgtacaaga gtctcctgat aaagaaggaa aggatgaaga attggaggac
     2161 gctattccac agtcaatgca agatttgcta tgtatgaata acgactttcc tccaagagca
     2221 cattgcctgg taagatggta tggcctgcgt gagtttgtgg tgattgctcc tgctgcacac
     2281 agtgatgcag ttctcagtga atccaagtgt aacctcctcc ttagttccat ttctattgct
     2341 ttgggaaaca ctggctgcca ggtaccactg tttgtccaaa tacatcacaa atggcgacga
     2401 atgtacatgg gagaatgtca aggtcctggt gttcgtactg actttgaaat ggttcatctt
     2461 cgaaaagtgc caagtcagta cactcatttg tcaggcctgc tggacatctt caaatccaaa
     2521 attggctgtc ctttaactcc attgcctcca gttagcattg cgattcggct cacatacgtg
     2581 cttcaggatt ggcagcaata cttttggcct cagcagcctc cagatataga tgcccttgtg
     2641 ggaggagaag ttgggggcct ggagttcggc aagttgccat ttggtgcctg tgaagatcct
     2701 atcagtgaac tccatttagc aacaacgtgg cctcatttga cagaagggat cattgtagat
     2761 aatgatgttt attctgattt ggatcctgtt caagctcccc attggtcggt tagagttcgg
     2821 aaagctgata atccccagtg cttactaggt gattttgtta ctgaattttt aaaaatttgc
     2881 cgtcgaaagg aatcaacgga tgaaattctt ggacgatcca catttgaaga agagggaaga
     2941 gaagttgctg atataactca tgctttgtca aagctgactg agccagcgcc agttccaatt
     3001 cataaattat cagtttcaaa tatggtacac actgcaaaga agaagatacg gaagcacaga
     3061 ggggaagagt cgccgctgaa cagtgatgtt ctcaatacta ttctcttgtt cttgttccca
     3121 gatgctgtct ctgagaagcc gttagatgga actacttcaa tagacaatag cattcctgca
     3181 ccagaggctg gagactatac tctctacaat cagttcaagt ctgcgccttc cgacagtcta
     3241 acatacaaac tggccttgtg tctatgtatg atcaacttct accatggagg gctgaaggga
     3301 gtggcacatc tctggcagga atttgttctt gaaatgcgtt tcagatggga aaacaacttt
     3361 ctgattccag gactagcaag tggatcacca gatctaagat gctgcttact gcatcagaaa
     3421 ctacagatgt taaattgttg tattgaaagg aagaaagcac gtgatgaagg gaaaaagacg
     3481 agcctctcgg atagtacgac tagtgcatat ccaggggatg ctgggaaaac tggaggccag
     3541 ctgggcctgg accatctcag agatacagag aaggaaaagg gagaagtagg gaagtcctgg
     3601 gattcctgga gtgatagtga agaagagttt tttgaatgcc tcagtgatac ggaagacctt
     3661 aaaggaaacg gacaggagag tggtaagaaa ggcggtccca aggagatggc aaacttaaag
     3721 ccagaaggcc gcctgcacca gcacgggaaa cttaccctgc tgcataatgg agagcctctc
     3781 tacattccag tgacccagga accagcaccc atgacagaag atctgctgga agagcagtct
     3841 gaggtcttag caaaattagg gacatctgct gagggggcgc acctccgcgc acgcatgcag
     3901 agtgcctgtc tcctctccga tatggagtct tttaaggcag ctaatccagg ctgctttctg
     3961 gaagatttcg tgaggtggta ctcaccaagg gactacattg aggaggaggt gactgacgag
     4021 aagggcaatg tggtcctgaa gggggagctg agtgcccgta tgaagatccc cagcaacatg
     4081 tgggtagaag cctgggagac tgctaagcct gtgccagcac gacgtcagag gagactgttt
     4141 gatgacaccc gagaggctga aaaggtcctg cactacctgg ccatgcagaa gcctgcagac
     4201 ctagccaggc acctcctgcc ttgtgtgatt catgcagctg ttctcaaggt aaaggaagaa
     4261 gaaagtctgg aaaacattcc ttctgttaag aagattataa agcagatcat agcccattcc
     4321 agcaaagttc tgcatttccc caatcctgag gacaagaaac tggaagaaat catccttcag
     4381 attactactg tggaagctat aatagcccga gcccggtccc tgaaggccaa atttggaact
     4441 gagaagtgtg agcacgagga ggaaaaggaa ggtctggaaa ggtttgtcag ctgcctgctg
     4501 gagcagcctg aggtatcagt cactggggca ggaagaggac atgctggccg catcatccac
     4561 aagctgtttg tgaatgctca gcgggctgcg gcagtggctc tgccagagga ggagcttaag
     4621 aaatcaggct gcccagagga gagaagacag accttggtgt cagacttccc accacctgca
     4681 ggccgggagc tgatcctgag ggccactgtg ccgcggcctg ctccctactc caaggccctg
     4741 ccacagcgta tgtacagcgt actcaccaag gaggacttca gactcgcggg tgccttttcc
     4801 tcagacacct cctttttcta ggcggccgcg actctagagt cgacggatct ttttccctct
     4861 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     4921 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     4981 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     5041 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     5101 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     5161 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     5221 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     5281 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     5341 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5401 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5461 gcccgctttc cagtcgggaa acctgtcgtg ccagcggata cgcatctcaa ttagtcagca
     5521 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5581 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5641 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5701 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5761 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5821 cttatcatgt ctcgatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6841 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6901 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6961 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7021 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7081 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7141 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7201 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7261 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7321 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7381 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7441 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7501 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7561 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7621 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7681 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7741 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7801 cacatttccc cgaaaa
//
