LOCUS       p201N-OE-A2:COR15A-HA       10865 bp    DNA     circular UNA 28-MAY-2021
DEFINITION  Gibson Assembly ligation product.
ACCESSION   urn.local...21-guwjwor
VERSION     urn.local...21-guwjwor
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..8613
                     /Source="referenceToDocument: urn:local:.:4f-guwjwps"
                     /label="p201N-OE-cor15A-ha minus 35S extraction
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="3664989381752bba83c11-a3d3-4c94-a88e-c3
                     5509cf3d84"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="3665214818394876a09bd-f266-4587-95aa-d0
                     d5732ea7ab"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="3665481984398cfd756c1-4f3e-462c-95be-62
                     a59200f99f"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="36657515968393dec16f8-90ef-4d2c-9883-f0
                     1c3f0466b0"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="3665843674619d6c9aec3-5048-4f51-9d8a-c9
                     0bb559b7eb"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="366628862777355dda717-3ce6-467c-8125-14
                     b4b453beae"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="3666385837365d564f4fb-736e-40bf-ab00-b6
                     f40a63ce07"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="3666849785044712fd30e-a2f2-4e36-a28b-d3
                     3c62ca89e8"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="366713110812472fd8ddf-0ead-47a2-8e7a-86
                     1e6c92bd74"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="36672157863324d04c9fe-74aa-4925-9e0b-11
                     04fa27a264"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="3667708465851bf23cc35-c7c9-4197-8582-5f
                     e9478bb3ea"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     3833..3852
                     /Hairpin="5.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="54.0"
                     /Self_Dimer_Tm="11.3"
                     /Tm="62.4"
                     /annotation_group="3667977448056e5ef0c57-78e7-4618-86fb-70
                     8e02b91048"
                     /Sequence="CGGAGACGGTCACAGCTTGT"
                     /created_by="primer3"
                     /label="pmeca49F"
     primer_bind     4027..4048
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="62.8"
                     /Self_Dimer_Tm="13.3"
                     /Tm="61.2"
                     /annotation_group="3664312623541f6b55fe6-1fea-4f1b-bc09-b6
                     a9462da224"
                     /Sequence="CGCACAGATGCGTAAGGAGAAA"
                     /created_by="primer3"
                     /label="pUC-R"
     misc_feature    complement(4128..4147)
                     /modified_by="User"
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.3"
                     /annotation_group="3664853189312e9098ac1-14f3-42ad-905c-65
                     2e24bd1bda"
                     /Sequence="ATTACCGCCTTTGAGTGAGC"
                     /created_by="primer3"
                     /label="VR"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6037..6062)
                     /Mismatches=0
                     /%GC="42.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="36642644597629f6e3e0f-7c68-4113-8ac4-a8
                     ce0713fa10"
                     /Sequence="GTTTACACCACAATATATCCTGCCAC"
                     /created_by="primer3"
                     /label="LB(out)"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     6037..6062
                     /Mismatches=0
                     /%GC="42.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="366837571310179ec655a-99f3-4816-8554-d6
                     ab1b532da4"
                     /Sequence="GTGGCAGGATATATTGTGGTGTAAAC"
                     /created_by="primer3"
                     /label="LB-1a"
     motif           6038..6063
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="TGGCAGGATATATTGTGGTGTAAACA"
                     /annotation_group="left border: 6,038 -> 6,063"
                     /label="left border"
     misc_feature    complement(6068..6096)
                     /Mismatches=0
                     /%GC="37.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.7"
                     /annotation_group="36644968820586493736d-6458-4fd0-aa29-d1
                     7e938e2d03"
                     /Sequence="GCAATGTGTTATTAAGTTGTCTAAGCGTC"
                     /created_by="primer3"
                     /label="LB(out)"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     6076..6101
                     /Mismatches=0
                     /%GC="42.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.4"
                     /annotation_group="36683342724884c55a2ac-7504-4c81-99b1-9a
                     0e570d9924"
                     /Sequence="GACAACTTAATAACACATTGCGGACG"
                     /created_by="primer3"
                     /label="LB(-58)R"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="3664945101265f5f146dd-e0f2-4cf8-8dc9-8e
                     bac50d360c"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="366525642953179c5421e-9b53-4303-8b84-d9
                     53ceb29047"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="366552841523390b5afad-4efd-4623-ada0-d1
                     86bd19f03b"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="3665935633659aa5472db-bcff-402a-b2a3-c4
                     e82bd4ece4"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="36660692783986ec8f86e-0b3e-4c4d-9847-1c
                     f5fac2bfb6"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="366647876800743cfeede-3459-4ecd-8233-30
                     0f335f3b34"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="36666138512787af5d410-8dab-4a7c-8931-63
                     648b4baad1"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="36668966361160945a23c-798a-4838-a1b6-6d
                     10fcdfb9b6"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="36673059963462ab7a7c3-9cfc-489d-8c59-ff
                     82c4ddf483"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="36674462543982413eee6-290a-4e2a-834c-25
                     44d459db08"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="3667667930232126a0899-305e-4f61-971e-64
                     3cc1c91024"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6228..6251
                     /Hairpin="10.0"
                     /Primer-Dimer="8.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="14.4"
                     /Tm="59.5"
                     /annotation_group="3667934937093dca953fe-2a6d-4da7-b07a-ba
                     f160b1cdad"
                     /Sequence="CGCAACGCAATTAATGTGAGTTAG"
                     /created_by="primer3"
                     /label="Pmeca741r"
     primer_bind     6290..6313
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.5"
                     /annotation_group="36636301903135da60b52-69ce-433a-a46d-cd
                     f179dd3393"
                     /Sequence="TCCGGCTCGTATGTTGTGTGGAAT"
                     /created_by="primer3"
                     /label="pGFPm-T158F"
     primer_bind     6290..6313
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.5"
                     /annotation_group="3663863749424698a6f74-e62c-41ba-814c-31
                     0da5efcf3a"
                     /Sequence="TCCGGCTCGTATGTTGTGTGGAAT"
                     /created_by="primer3"
                     /label="pGFPmT(158)F"
     primer_bind     6290..6313
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.5"
                     /annotation_group="3663909859620c167ed1d-d88f-4d89-be09-1a
                     1b1f27f455"
                     /Sequence="TCCGGCTCGTATGTTGTGTGGAAT"
                     /created_by="primer3"
                     /label="pBI101Pseq(-50)F"
     primer_bind     6317..6340
                     /Mismatches=0
                     /%GC="45.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /annotation_group="3664811043190b7c48e75-b8c8-4177-97dd-a4
                     02bd87cc9e"
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /created_by="primer3"
                     /label="M13-R (-46)"
     primer_bind     6318..6341
                     /Hairpin="4.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.8"
                     /annotation_group="3664675965977052eceb2-65ee-42f2-9893-d8
                     2e220b87b4"
                     /Sequence="AGCGGATAACAATTTCACACAGGA"
                     /created_by="primer3"
                     /label="M13-48-R"
     primer_bind     6318..6341
                     /Hairpin="4.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.8"
                     /annotation_group="366472257098910b436a0-3c4d-4127-a979-36
                     8916f6ffdd"
                     /Sequence="AGCGGATAACAATTTCACACAGGA"
                     /created_by="primer3"
                     /label="M13-48-R"
     primer_bind     6318..6340
                     /Mismatches=0
                     /%GC="43.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.6"
                     /annotation_group="36637747265722592e0d2-8252-47c1-9bcd-d9
                     02db3000da"
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /created_by="primer3"
                     /label="M13(pORE4)R "
     primer_bind     6337..6353
                     /Mismatches=0
                     /%GC="47.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="49.1"
                     /annotation_group="36647654591196f41f08c-57d9-400d-b49c-ab
                     b282707f30"
                     /Sequence="CAGGAAACAGCTATGAC"
                     /created_by="primer3"
                     /label="M13-R (-26)"
     terminator      complement(6393..6798)
                     /%_Identity=100
                     /Motif="TGTCCTATCAGTTTTCTCTTTTTGTCGAACGGTAATTTAGAGTTTTTTTT
                     GCTATATGGATTTTCGTTTTTGATGTATGTGACAACCCTCGGGATTGTTGATTTATTT
                     CAAAACTAAGAGTTTTTGCTTATTGTTCTCGTCTATTTTGGATATCAATCTTAGTTTT
                     ATATCTTTTCTAGTTCTCTACGTGTTAAATGTTCAACACACTAGCAATTTGGCTGCAG
                     CGTATGGATTATGGAACTATCAAGTCTGTGGGATCGATAAATATGCTTCTCAGGAATT
                     TGAGATTTTACAGTCTTTATGCTCATTGGGTTGAGTATAATATAGTAAAAAAATAGTA
                     AATTTAAGCAATAATGTTAGGTGCTATGTGTCTGTCGAGACTATTGGGGTACGGCGCG
                     GCTTAATT"
                     /annotation_group="StUbi terminator: 6,393 <- 6,798"
                     /modified_by="mariaortega1old"
                     /label="StUbi terminator"
     gene            complement(6822..7616)
                     /%_Identity=100
                     /Motif="TGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGAC
                     TGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGG
                     GGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGA
                     CGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTC
                     GACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG
                     ATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAAT
                     GCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACAT
                     CGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGG
                     ACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCAT
                     GCCCGACGGCGATGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATG
                     GTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACC
                     GCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATG
                     GGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCC
                     TTCTATCGCCTTCTTGACGAGTTCTTCTGAGAATTCGGCTTGATCCAAA"
                     /annotation_group="nptII: 6,822 <- 7,616"
                     /modified_by="mariaortega1old"
                     /label="nptII"
     primer_bind     6844..6863
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="56.5"
                     /annotation_group="3663582001904179bccc2-4b84-4f9f-8ecf-79
                     050f093b12"
                     /Sequence="GAAGAACTCGTCAAGAAGGC"
                     /created_by="primer3"
                     /label="NPT2"
     primer_bind     6954..6977
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="48.9"
                     /Self_Dimer_Tm="12.3"
                     /Tm="63.8"
                     /annotation_group="3663997482918993fb5fc-bfb9-4b4e-aa0d-78
                     7c804a5514"
                     /Sequence="TATCACGGGTAGCCAACGCTATGT"
                     /created_by="primer3"
                     /label="NPT(682)R"
     primer_bind     6962..6985
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="48.9"
                     /Self_Dimer_Tm="None"
                     /Tm="60.9"
                     /annotation_group="3663726415315cf4a59a7-df1a-42be-ad96-e3
                     2235add381"
                     /Sequence="GTAGCCAACGCTATGTCCTGATAG"
                     /created_by="primer3"
                     /label="NPTII(R)QD "
     misc_feature    complement(7296..7319)
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="48.7"
                     /Self_Dimer_Tm="14.6"
                     /Tm="64.0"
                     /annotation_group="366395536978783189885-d098-4804-bb38-7a
                     83998e6892"
                     /Sequence="ACCTTGCTCCTGCCGAGAAAGTAT"
                     /created_by="primer3"
                     /label="NPT(317)F"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     7548..7571
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="30.2"
                     /Self_Dimer_Tm="None"
                     /Tm="63.6"
                     /annotation_group="36640792639922751fe1d-4d2b-408a-ac6f-ee
                     d4715f30f9"
                     /Sequence="CGATTGTCTGTTGTGCCCAGTCAT"
                     /created_by="primer3"
                     /label="RB472R0"
     misc_feature    complement(7567..7588)
                     /Mismatches=0
                     /%GC="54.5"
                     /Hairpin_Tm="50.3"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="366367840405706c6881b-fcb6-4d89-9853-2f
                     edfb768743"
                     /Sequence="GGAGAGGCTATTCGGCTATGAC"
                     /created_by="primer3"
                     /label="NPTII(F)QD "
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     7607..7630
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.2"
                     /annotation_group="36641262883881fdefdbc-f262-4caa-9a50-dd
                     467fca0784"
                     /Sequence="CCTGCGTGCAATCCATCTTGTTCA"
                     /created_by="primer3"
                     /label="RB416R2"
     misc_feature    complement(7610..7629)
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="33.3"
                     /Self_Dimer_Tm="None"
                     /Tm="58.1"
                     /annotation_group="36635294734209b9f1574-dce9-463e-a57f-5e
                     36870f0d06"
                     /Sequence="GAACAAGATGGATTGCACGC"
                     /created_by="primer3"
                     /label="NPT-1"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7610..7629)
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="33.3"
                     /Self_Dimer_Tm="None"
                     /Tm="58.1"
                     /annotation_group="3664583144499ccfe4520-a8ac-4723-8f00-66
                     ad102780f4"
                     /Sequence="GAACAAGATGGATTGCACGC"
                     /created_by="primer3"
                     /label="NPT1"
                     /note="Geneious type: primer_bind_reverse"
     promoter        complement(7650..8576)
                     /%_Identity=100
                     /Motif="TCGATTTAAATTTCATCGAAGAGATTAATATCGAATAATCATATACATAC
                     TTTAAATACATAACAAATTTTAAATACATATATCTGGTATATAATTAATTTTTTAAAG
                     TCATGAAGTATGTATCAAATACACATATGGAAAAAATTAACTATTCATAATTTAAAAA
                     ATAGAAAAGATACATCTAGTGAAATTAGGTGCATGTATCAAATACATTAGGAAAAGGG
                     CATATATCTTGATCTAGATAATTAACGATTTTGATTTATGTATAATTTCCAAATGAAG
                     GTTTATATCTACTTCAGAAATAACAATATACTTTTATCAGAACATTCAACAAAGTAAC
                     AACCAACTAGAGTGAAAAATACACATTGTTCTCTAGACATACAAAATTGAGAAAAGAA
                     TCTCAAAATTTAGAGAAACAAATCTGAATTTCTAGAAGAAAAAAATAATTATGCACTT
                     TGCTATTGCTCGAAAAATAAATGAAAGAAATTAGACTTTTTTAAAAGATGTTAGACTA
                     GATATACTCAAAAGCTATTAAAGGAGTAATATTCTTCTTACATTAAGTATTTTAGTTA
                     CAGTCCTGTAATTAAAGACACATTTTAGATTGTATCTAAACTTAAATGTATCTAGAAT
                     ACATATATTTGAATGCATCATATACATGTATCCGACACACCAATTCTCATAAAAAACG
                     TAATATCCTAAACTAATTTATCCTTCAAGTCAACTTAAGCCCAATATACATTTTCATC
                     TCTAAAGGCCCAAGTGGCACAAAATGTCAGGCCCAATTACGAAGAAAAGGGCTTGTAA
                     AACCCTAATAAAGTGGCACTGGCAGAGCTTACACTCTCATTCCATCAACAAAGAAACC
                     CTAAAAGCCGCAGCGCCACTGATTTCTCTCCTCCAGGCGAAGGATCGATCTTCGAATG
                     CATCGCG"
                     /annotation_group="u6: 7,650 <- 8,576"
                     /modified_by="mariaortega1old"
                     /label="StUbi"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="36650356368828ebc5409-50b3-4570-8cb3-10
                     311768bf91"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="36651738355236064fe23-bfb2-4a2e-84dd-f0
                     5d936bc32e"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="36654366573233fa311ec-9028-4a42-9997-fa
                     d4164d6af2"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="3665708804637798dfe9d-d02e-4ab4-a7d2-8f
                     dc0bf6a045"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="366611717830913625429-4813-48e7-96e6-70
                     aec5460210"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="3666247053181d0d5c89a-4daf-440e-a3ed-3b
                     db2e9af563"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="36666602544003e001045-760a-4bad-b2a1-c0
                     a589fea9da"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="36668021725551558c7d3-f876-4517-a8c0-43
                     3f99646053"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="3667083720089ba3d7e38-9e14-4455-a660-af
                     ca6816213e"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="3667491871429ca7872db-61db-45d6-987b-47
                     b847668b9b"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="36677532207274c6a504d-09dd-4492-acdb-40
                     dc79b11877"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7731..7751)
                     /Hairpin="6.0"
                     /Primer-Dimer="1.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="45.3"
                     /Self_Dimer_Tm="None"
                     /Tm="56.4"
                     /annotation_group="36680226631103ed27332-b1ad-4cda-a209-21
                     8926b1472f"
                     /Sequence="GCAGAGCTTACACTCTCATTC"
                     /created_by="primer3"
                     /label="ubiqF"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="36651281988442976d3e6-7348-41ea-a80a-61
                     ccc047cc40"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="36653476544692f6363fd-7738-41be-82e0-ad
                     df7d8daa87"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="366561604048457802eee-240b-492b-8e12-76
                     154780d674"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="3665795842882607fd576-345e-4737-940d-7b
                     3fa925adff"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="36661603485404dc4d48b-0581-4c93-aac7-66
                     51b47c442a"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="3666338864336249c80b7-5f71-4a4a-b73f-2a
                     6429174b88"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="3666705677167986efe81-f833-4259-b283-ba
                     f3c59cb7a5"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="366699138559651be6b91-a92a-48ec-9d60-1d
                     44ab747a8d"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="3667171188441358e88e4-6fb8-403f-add7-05
                     c1de2efe67"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="36675354270089ee1cbe0-ed17-4d34-9efd-af
                     1e983f460d"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="3667840919663fa799fde-5260-4092-b478-d2
                     c948c90dba"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8399
                     /Hairpin="5.0"
                     /Primer-Dimer="3.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="36.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.6"
                     /annotation_group="3668113530205d56917e5-4516-46cf-a5e2-bd
                     6ec59cab04"
                     /Sequence="ACATGCACCTAATTTCACTAGATGT"
                     /created_by="primer3"
                     /label="Ubi3p 218R"
     primer_bind     8375..8398
                     /Mismatches=0
                     /%GC="37.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.1"
                     /annotation_group="36644515802100e9e397c-cc17-42fa-8b8f-82
                     aaeebb4fee"
                     /Sequence="ACATGCACCTAATTTCACTAGATG"
                     /created_by="primer3"
                     /label="StUbiP-R"
     primer_bind     8375..8398
                     /Mismatches=0
                     /%GC="37.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.1"
                     /annotation_group="41663028888901c151f91e-fdef-4875-9cce-e
                     80ebdf0fdb0"
                     /Sequence="ACATGCACCTAATTTCACTAGATG"
                     /created_by="primer3"
                     /label="StUbiP-R"
     misc_feature    complement(8537..8567)
                     /Mismatches=0
                     /%GC="25.8"
                     /Hairpin_Tm="44.5"
                     /Self_Dimer_Tm="15.6"
                     /Tm="57.3"
                     /annotation_group="366864350332377425e00-952e-4458-b698-38
                     ea93e7791a"
                     /Sequence="ATTTCATCGAAGAGATTAATATCGAATAATC"
                     /created_by="primer3"
                     /label="p201N-SwR"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     8584..8603
                     /Mismatches=0
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="24.0"
                     /Tm="59.3"
                     /annotation_group="36644091211918dd96c64-e637-4bc2-a552-ea
                     bbda315d35"
                     /Sequence="GTGCTTTGGATCGATCTGCC"
                     /created_by="primer3"
                     /label="p201NC-F "
     ligation        8586..8605
                     /label="Ligation"
     misc_feature    join(<9947..10733,8586..>8613)
                     /Source="referenceToDocument: urn:local:.:4g-guwjwps"
                     /label="Digestion of Ligation of COR15A+HA(green) into PCM
                     Nos-35S extraction (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        8594..8613
                     /label="Ligation"
     misc_feature    8594..9966
                     /Source="referenceToDocument: urn:local:.:4i-guwjwps"
                     /label="pAtA2pt-Ppo - AtAct2 Promoter (concatenated
                     sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     motif           8594..8638
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="tcgatctgcccactagtgagTCATTATGTAAGAAAGTTTTGACGA"
                     /annotation_group="atactin2-F: 8,594 -> 8,638"
                     /label="atactin2-F"
     misc_feature    8606..8613
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     source          join(<8606..>8613,<10429..>10716)
                     /organism="Binary vector pCAMBIA-1302"
                     /mol_type="other DNA"
                     /db_xref="taxon:118390"
                     /label="source Binary vector pCAMBIA-1302"
     misc_feature    join(10429..>10716,<8606..>8613)
                     /Source="referenceToDocument: urn:local:.:4h-guwjwps"
                     /label="PCM Nos-35S (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     motif           8614..9946
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="tcattatgtaagaaagttttgacgaatatggcacgacaaaatggctagac
                     tcgatgtaattggtatctcaactcaacattatacttataccaaacattagttagacaa
                     aatttaaacaactattttttatgtatgcaagagtcagcatatgtataattgattcaga
                     atcgttttgacgagttcggatgtagtagtagccattatttaatgtacatactaatcgt
                     gaatagtgaatatgatgaaacattgtatcttattgtataaatatccataaacacatca
                     tgaaagacactttctttcacggtctgaattaattatgatacaattctaatagaaaacg
                     aattaaattacgttgaattgtatgaaatctaattgaacaagccaaccacgacgacgac
                     taacgttgcctggattgactcggtttaagttaaccactaaaaaaacggagctgtcatg
                     taacacgcggatcgagcaggtcacagtcatgaagccatcaaagcaaaagaactaatcc
                     aagggctgagatgattaattagtttaaaaattagttaacacgagggaaaaggctgtct
                     gacagccaggtcacgttatctttacctgtggtcgaaatgattcgtgtctgtcgatttt
                     aattatttttttgaaaggccgaaaataaagttgtaagagataaacccgcctatataaa
                     ttcatatattttcctctccgctttgaattgtctcgttgtcctcctcactttcatcagc
                     cgttttgaatctccggcgacttgacagagaagaacaaggaagaagactaagagagaaa
                     gtaagagataatccaggagattcattctccgttttgaatcttcctcaatctcatcttc
                     ttccgctctttctttccaaggtaataggaactttctggatctactttatttgctggat
                     ctcgatcttgttttctcaatttccttgagatctggaattcgtttaatttggatctgtg
                     aacctccactaaatcttttggttttactagaatcgatctaagttgaccgatcagttag
                     ctcgattatagctaccagaatttggcttgaccttgatggagagatccatgttcatgtt
                     acctgggaaatgatttgtatatgtgaattgaaatctgaactgttgaagttagattgaa
                     tctgaacactgtcaatgttagattgaatctgaacactgtttaaggttagatgaagttt
                     gtgtatagattcttcgaaactttaggatttgtagtgtcgtacgttgaacagaaagcta
                     tttctgattcaatcagggtttatttgactgtattgaactctttttgtgtgtttgcagc
                     tcataaa"
                     /annotation_group="AtAct2 Promoter: 424 -> 1,756"
                     /label="AtAct2 Promoter"
     primer_bind     8614..8638
                     /%GC="28.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Tm="55.3"
                     /Sequence="TCATTATGTAAGAAAGTTTTGACGA"
                     /created_by="primer3"
                     /Product_Size=1333
                     /annotation_group="63096589244966e0e37989-2250-4c3b-a054-f
                     e6ba5edf5e0pAtA2pt-Ppo - AtAct2 Promoter"
                     /Extension="TCGATCTGCCCACTAGTGAG"
                     /label="pAtA2pt-Ppo - AtAct2 Promoter (1 F) -
                     p201N-OE-cor15A-ha minus 35S"
     misc_feature    complement(9926..9946)
                     /%GC="38.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.0"
                     /Pair_Dimer_Tm="None"
                     /Tm="56.7"
                     /Sequence="TTTATGAGCTGCAAACACACA"
                     /created_by="primer3"
                     /Product_Size=1333
                     /annotation_group="63096725102738fc44e32d-f587-463e-9dab-d
                     e3010e0cfe1pAtA2pt-Ppo - AtAct2 Promoter"
                     /Extension="AAGACATCGCCATGGTCAAG"
                     /label="pAtA2pt-Ppo - AtAct2 Promoter (1333 R) -
                     p201N-OE-cor15A-ha minus 35S"
                     /note="Geneious type: primer_bind_reverse"
     motif           complement(9926..9966)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="aagacatcgccatggtcaagTTTATGAGCTGCAAACACACA"
                     /annotation_group="atactin2-cor15b R: 9,926 <- 9,966"
                     /label="atactin2-cor15b R"
     motif           9927..9966
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTGTGTTTGCAGCTCATAAACTTGACCATGGCGATGTCTT"
                     /annotation_group="atactin2-F: 9,927 -> 9,966"
                     /label="atactin2-F"
     ligation        9947..9966
                     /label="Ligation"
     misc_feature    9947..10865
                     /Source="referenceToDocument: urn:local:.:4f-guwjwps"
                     /label="p201N-OE-cor15A-ha minus 35S extraction
                     (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <9947..10446
                     /Source="referenceToDocument: urn:local:.:4j-guwjwps"
                     /label="COR15A+HA(green) (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     gene            9954..10400
                     /created_by="mariaortega1old"
                     /label="cor15A"
     primer_bind     9954..9974
                     /Pair_Dimer_Tm="None"
                     /Product_Size=467
                     /Mismatches=0
                     /Extension="GAGAGAACACGGGGGACT"
                     /%GC="47.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.2"
                     /annotation_group="3668470663162104f2ab5-22e6-47ee-92df-05
                     fb43da648e"
                     /Sequence="ATGGCGATGTCTTTCTCAGGA"
                     /created_by="primer3"
                     /label="COR15A+HA(green) (1 F) - PCM Nos-35S"
     adapter         10398..10420
                     /created_by="mariaortega1old"
                     /label="Nost2 adapter"
     misc_feature    complement(10399..10420)
                     /Pair_Dimer_Tm="None"
                     /Product_Size=467
                     /Mismatches=0
                     /Extension="TTGCCAAATGTTTGAACG"
                     /%GC="45.5"
                     /Hairpin_Tm="30.8"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="366851237445642a999ad-e184-4f3c-b4c0-2d
                     b88ac0cb4b"
                     /Sequence="AATTCGAGCTGGTCACCAATCT"
                     /created_by="primer3"
                     /label="COR15A+HA(green) (467 R) - PCM Nos-35S"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    10401..10428
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(10409..10429)
                     /Mismatches=0
                     /%GC="57.1"
                     /Hairpin_Tm="38.5"
                     /Self_Dimer_Tm="None"
                     /Tm="61.1"
                     /annotation_group="36638178079153a87fb57-5887-47db-a9d8-86
                     36ab88c419"
                     /Sequence="GATCGGGGAAATTCGAGCTGG"
                     /created_by="primer3"
                     /label="pC1302(786)R"
                     /note="Geneious type: primer_bind_reverse"
     source          <10421..>10428
                     /organism="Binary vector pCAMBIA-1302"
                     /mol_type="other DNA"
                     /db_xref="taxon:118390"
                     /label="source Binary vector pCAMBIA-1302"
     motif           <10421..10423
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="ATTGGTGACCAGCTCGAATTTCC"
                     /annotation_group="ATTGGTGACCAGCTCGAATTTCC: 7 -> 29"
                     /label="ATTGGTGACCAGCTCGAATTTCC"
     misc_feature    <10421..>10428
                     /label="Digestion of pCambia 1302 (modified) - Fragment 2
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    10429..>10716
                     /note="nos (nopaline synthase) 3'UTR (polyA signal)"
                     /label="misc"
     ligation        10429..10446
                     /label="Ligation"
     misc_feature    complement(10468..10487)
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="3663483268833a5527c15-b43b-4fbe-9306-8c
                     71d1bbedcc"
                     /Sequence="ATCGCAAGACCGGCAACAGG"
                     /created_by="primer3"
                     /label="NOST2"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10468..10487)
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="3664173003571d3254385-ae1b-4f5a-90b3-95
                     c793580cc5"
                     /Sequence="ATCGCAAGACCGGCAACAGG"
                     /created_by="primer3"
                     /label="NOST2"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10468..10487)
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="3668293382837b9cfc2c2-0dcb-4b1a-be11-8b
                     9f76b60a48"
                     /Sequence="ATCGCAAGACCGGCAACAGG"
                     /created_by="primer3"
                     /label="NOST2"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10468..10487)
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="42047299047783cf803c38-504a-4ef1-86b9-b
                     913dbb652c9"
                     /Sequence="ATCGCAAGACCGGCAACAGG"
                     /created_by="primer3"
                     /label="NOST2"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10469..10492)
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.7"
                     /annotation_group="366403843412023baaefa-8dde-4602-bd54-9d
                     331745ff2e"
                     /Sequence="TAATCATCGCAAGACCGGCAACAG"
                     /created_by="primer3"
                     /label="NOST-pCM1302(849)R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10469..10492)
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.7"
                     /annotation_group="366435916204824809cc3-bed3-43f0-87ad-fb
                     70eb762539"
                     /Sequence="TAATCATCGCAAGACCGGCAACAG"
                     /created_by="primer3"
                     /label="NOST-pCM1302(849)R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     10472..10492
                     /Mismatches=0
                     /%GC="47.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.9"
                     /Tm="60.4"
                     /annotation_group="366825165050244b6aeac-85d6-4c45-97e9-b6
                     1b1b030ecf"
                     /Sequence="TTGCCGGTCTTGCGATGATTA"
                     /created_by="primer3"
                     /label="NOST2(47)F"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="366508079354346229593-5142-4c38-862b-62
                     75e6e6c8e5"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="366539467479270740b70-e181-4c7c-b237-6d
                     deec657ce0"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="36656628155365f80ea77-381e-4cdb-bc5b-72
                     5c42250832"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="36660238882940a7a5ac3-e454-450c-88a9-17
                     7c0ae5f2f8"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="366620303843848612015-a229-40f1-a87d-ab
                     f02eba50ea"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="3666570494357df1355f2-9fb6-4039-9c69-df
                     5f241310fd"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="3666754793434e3a694cd-b9ae-45d2-955a-f1
                     f11d8c506a"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="36670363753660dfcc189-2d22-43df-bdae-c0
                     99a7a59337"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="3667400682898a74657b2-39f2-498b-802c-1f
                     87f0385ea6"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="3667576612927a4ad1ee5-c677-4a25-9a66-1e
                     2f9d0eb2ae"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="3667795188613834fc730-0873-40d4-b08d-2d
                     44846c061b"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10477..10495)
                     /Hairpin="7.0"
                     /Primer-Dimer="0.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /%GC="42.1"
                     /Hairpin_Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /annotation_group="36680711562919d8bec66-98e1-4134-81b4-3f
                     c81f1a72db"
                     /Sequence="TGATAATCATCGCAAGACC"
                     /created_by="primer3"
                     /label="NosT-rev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10661..10709)
                     /Sequence="CCTGCAGGTCGACTCTAGAGAATACGGATCCGATCTAGTAACATAGA
                     TG"
                     /Mismatches=0
                     /annotation_group="aa65d57a-e675-420d-b38d-a0e4e2359742"
                     /label="PCM BamHI Nos R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    10682..10716
                     /Original_Bases="AA"
                     /label="AA"
                     /note="Geneious type: Editing History Replacement"
     motif           complement(10690..10716)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="AAACTGACCTGCAGGTCGACTCTAGAG"
                     /annotation_group="r: 1,290 <- 1,316"
                     /label="r"
     misc_feature    complement(10697..10716)
                     /%GC="55.0"
                     /Hairpin_Tm="61.2"
                     /Self_Dimer_Tm="44.8"
                     /Pair_Dimer_Tm="None"
                     /Tm="60.3"
                     /Sequence="AAACTGACCTGCAGGTCGAC"
                     /created_by="primer3"
                     /Product_Size=1316
                     /annotation_group="69433627695213b81da76c-cef9-4008-a500-d
                     7866ced9b73Digestion of Ligation of COR15A+HA(green) into
                     PCM Nos-35S extraction"
                     /Extension="CTGTCAAACACTGATAGTTT"
                     /label="Digestion of Ligation of COR15A+HA(green) into PCM
                     Nos-35S extraction (1316 R) - Digestion of p201N Cas9 TBJ
                     - Fragment 2"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10697..10716)
                     /Pair_Dimer_Tm="None"
                     /Product_Size=1316
                     /Mismatches=0
                     /Extension="CTGTCAAACACTGATAGTTT"
                     /%GC="55.0"
                     /Hairpin_Tm="61.2"
                     /Self_Dimer_Tm="44.8"
                     /Tm="60.3"
                     /annotation_group="366869046101559ce5198-b9d6-4ba3-a6a8-2e
                     1cde213eec"
                     /Sequence="AAACTGACCTGCAGGTCGAC"
                     /created_by="primer3"
                     /label="Digestion of Ligation of COR15A+HA(green) into PCM
                     Nos-35S extraction (1316 R) - Digestion of p201N Cas9 TBJ
                     - Fragment 2 2"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(10697..10716)
                     /Pair_Dimer_Tm="None"
                     /Product_Size=1316
                     /Mismatches=0
                     /Extension="AACACTGATAGTTTAAACTGA"
                     /%GC="55.0"
                     /Hairpin_Tm="61.2"
                     /Self_Dimer_Tm="44.8"
                     /Tm="60.3"
                     /annotation_group="3668779500195f57f9e2b-74b4-47a5-baa2-72
                     c4ced18ee5"
                     /Sequence="AAACTGACCTGCAGGTCGAC"
                     /created_by="primer3"
                     /label="Digestion of Ligation of COR15A+HA(green) into PCM
                     Nos-35S extraction (1316 R) - Digestion of p201N Cas9 TBJ
                     - Fragment 2"
                     /note="Geneious type: primer_bind_reverse"
     ligation        <10717..10733
                     /label="Ligation"
     misc_feature    <10717..8605
                     /Source="referenceToDocument: urn:local:.:4e-guwjwps"
                     /label="Digestion of p201N Cas9 TBJ - Fragment 2
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     motif           complement(10728..10752)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="GTTTACCCGCCAATATATCCTGTCA"
                     /annotation_group="right border: 14,213 <- 14,237"
                     /label="right border"
     primer_bind     10729..10747
                     /Mismatches=0
                     /%GC="52.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.9"
                     /annotation_group="3664542436034ae536e85-9ed5-4d31-b741-f1
                     9708607228"
                     /Sequence="GACAGGATATATTGGCGGG"
                     /created_by="primer3"
                     /label="RB(out)"
ORIGIN      
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg caggatcatg
     4921 aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     4981 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     5041 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     5101 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     5161 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     5221 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     5281 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     5341 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     5401 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     5461 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     5521 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     5581 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     5641 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     5701 taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     5761 actgtttatg taagcagaca gttttattgt tcatgatctg gatcacaggc agcaacgctc
     5821 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     5881 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     5941 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6001 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6061 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6121 acgccgaatt gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6181 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6241 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6301 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     6361 cgaattcctt gactagaggg taggcgcggc ttaattaagc cgcgccgtac cccaatagtc
     6421 tcgacagaca catagcacct aacattattg cttaaattta ctattttttt actatattat
     6481 actcaaccca atgagcataa agactgtaaa atctcaaatt cctgagaagc atatttatcg
     6541 atcccacaga cttgatagtt ccataatcca tacgctgcag ccaaattgct agtgtgttga
     6601 acatttaaca cgtagagaac tagaaaagat ataaaactaa gattgatatc caaaatagac
     6661 gagaacaata agcaaaaact cttagttttg aaataaatca acaatcccga gggttgtcac
     6721 atacatcaaa aacgaaaatc catatagcaa aaaaaactct aaattaccgt tcgacaaaaa
     6781 gagaaaactg ataggacatt tgctaaacat taaaatcaaa atttggatca agccgaattc
     6841 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     6901 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     6961 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     7021 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     7081 gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     7141 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     7201 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     7261 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     7321 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     7381 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     7441 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     7501 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     7561 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     7621 atcttgttca atcatctcga gacgtaccgc gcgatgcatt cgaagatcga tccttcgcct
     7681 ggaggagaga aatcagtggc gctgcggctt ttagggtttc tttgttgatg gaatgagagt
     7741 gtaagctctg ccagtgccac tttattaggg ttttacaagc ccttttcttc gtaattgggc
     7801 ctgacatttt gtgccacttg ggcctttaga gatgaaaatg tatattgggc ttaagttgac
     7861 ttgaaggata aattagttta ggatattacg ttttttatga gaattggtgt gtcggataca
     7921 tgtatatgat gcattcaaat atatgtattc tagatacatt taagtttaga tacaatctaa
     7981 aatgtgtctt taattacagg actgtaacta aaatacttaa tgtaagaaga atattactcc
     8041 tttaatagct tttgagtata tctagtctaa catcttttaa aaaagtctaa tttctttcat
     8101 ttatttttcg agcaatagca aagtgcataa ttattttttt cttctagaaa ttcagatttg
     8161 tttctctaaa ttttgagatt cttttctcaa ttttgtatgt ctagagaaca atgtgtattt
     8221 ttcactctag ttggttgtta ctttgttgaa tgttctgata aaagtatatt gttatttctg
     8281 aagtagatat aaaccttcat ttggaaatta tacataaatc aaaatcgtta attatctaga
     8341 tcaagatata tgcccttttc ctaatgtatt tgatacatgc acctaatttc actagatgta
     8401 tcttttctat tttttaaatt atgaatagtt aattttttcc atatgtgtat ttgatacata
     8461 cttcatgact ttaaaaaatt aattatatac cagatatatg tatttaaaat ttgttatgta
     8521 tttaaagtat gtatatgatt attcgatatt aatctcttcg atgaaattta aatcgataac
     8581 tatgtgcttt ggatcgatct gcccactagt gagtcattat gtaagaaagt tttgacgaat
     8641 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
     8701 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
     8761 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
     8821 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
     8881 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
     8941 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
     9001 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
     9061 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
     9121 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
     9181 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
     9241 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
     9301 attcatatat tttcctctcc gctttgaatt gtctcgttgt cctcctcact ttcatcagcc
     9361 gttttgaatc tccggcgact tgacagagaa gaacaaggaa gaagactaag agagaaagta
     9421 agagataatc caggagattc attctccgtt ttgaatcttc ctcaatctca tcttcttccg
     9481 ctctttcttt ccaaggtaat aggaactttc tggatctact ttatttgctg gatctcgatc
     9541 ttgttttctc aatttccttg agatctggaa ttcgtttaat ttggatctgt gaacctccac
     9601 taaatctttt ggttttacta gaatcgatct aagttgaccg atcagttagc tcgattatag
     9661 ctaccagaat ttggcttgac cttgatggag agatccatgt tcatgttacc tgggaaatga
     9721 tttgtatatg tgaattgaaa tctgaactgt tgaagttaga ttgaatctga acactgtcaa
     9781 tgttagattg aatctgaaca ctgtttaagg ttagatgaag tttgtgtata gattcttcga
     9841 aactttagga tttgtagtgt cgtacgttga acagaaagct atttctgatt caatcagggt
     9901 ttatttgact gtattgaact ctttttgtgt gtttgcagct cataaacttg accatggcga
     9961 tgtctttctc aggagctgtt ctcactggta tggcttcttc tttccacagc ggagccaagc
    10021 agagcagctt cggcgctgtc agagtcggcc agaaaactca gttcgtcgtc gtttctcaac
    10081 gcaagaagtc gttgatctac gccgctaaag gtgacggcaa catcctcgat gacctcaatg
    10141 aggccacaaa gaaagcttca gatttcgtga cggataaaac aaaagaggca ttagcagatg
    10201 gtgagaaagc gaaagactac gttgttgaaa aaaacagtga aaccgcagat acattgggta
    10261 aagaagctga gaaagctgcg gcgtatgtgg aggagaaagg aaaagaagcc gcaaacaagg
    10321 cggcagagtt cgcggagggt aaagcaggag aggctaagga tgccacaaag tacccttacg
    10381 atgtgccaga ctacgcttag attggtgacc agctcgaatt tccccgatcg ttcaaacatt
    10441 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
    10501 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
    10561 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
    10621 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
    10681 atccgtattc tctagagtcg acctgcaggt cagtttctat cagtgtttga caggatatat
    10741 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt
    10801 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
    10861 atcaa
//
