LOCUS       LentiX_((203-bp-       10043 bp    DNA     circular SYN 20-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10043
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     CDS             6..50
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    317..433
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     polyA_signal    complement(460..684)
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(696..1415)
                     /codon_start=1
                     /label=mGreenLantern
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     regulatory      1412..1421
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    complement(1427..1481)
                     /label=YBTATA-extra_fromZF43/ZF37_hybridpromoter
     promoter        1456..1480
                     /label=YB_TATA minimal promoter
     promoter        complement(1462..1478)
                     /label=minP (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=53.1"
                     /note="/other=promoter"
     protein_bind    complement(1504..1537)
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     protein_bind    complement(1659..1678)
                     /label=attB1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=80.0"
                     /note="/other=protein_bind"
     misc_feature    complement(1680..1713)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=64.2"
                     /note="/other=misc_feature"
     protein_bind    complement(1741..1774)
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     protein_bind    1797..1805
                     /label=ZF37 Binding Site
                     /bound_moiety="ZF37"
     protein_bind    1801..1809
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1812..1820
                     /label=ZF37 Binding Site
                     /bound_moiety="ZF37"
     protein_bind    1816..1824
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1827..1835
                     /label=ZF37 Binding Site
                     /bound_moiety="ZF37"
     protein_bind    1831..1839
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1842..1850
                     /label=ZF37 Binding Site
                     /bound_moiety="ZF37"
     protein_bind    1846..1854
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1857..1865
                     /label=ZF37 Binding Site
                     /bound_moiety="ZF37"
     protein_bind    1861..1869
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     protein_bind    1872..1880
                     /label=ZF37 Binding Site
                     /bound_moiety="ZF37"
     protein_bind    1876..1884
                     /label=ZF43 Binding Site
                     /bound_moiety="ZF43"
     misc_feature    2197..2199
                     /label=XbaI
     promoter        2208..3389
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     promoter        2226..2437
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          2438..3380
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     intron          2438..2551
                     /label=EF-1-alpha intron A (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=95.6"
                     /note="/match_length=12.4"
                     /note="/other=intron"
     intron          3177..3380
                     /label=EF-1-alpha intron A
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     promoter        3389..3423
                     /label=TRE3GV promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=10.0"
                     /note="/other=promoter"
     regulatory      3445..3454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3451..4386
                     /codon_start=1
                     /label=iRFP670
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     misc_feature    4397..4985
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(4868..4879)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5077..5310
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5382..5503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5419..5438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5473..5492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5543..5678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5605..5624
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(5698..5717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5699..5717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5727..5744)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     complement(5727..5743)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5736..5758)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5885..6340
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5972..5991)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6182..6203
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6366..6470
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6471..7331
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6689..6708)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7502..8090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7991..8010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8244..8261
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8378..8399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8414..8444
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8452..8468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8457..8479
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8476..8492
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8476..8492
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8511..8531
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8513..8531
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8559..8785
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8786..8966
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9013..9138
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    9631..9864
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
       61 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
      121 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
      181 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
      241 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
      301 gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      361 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      421 aattcaaaat tttatcgtcc agtgtggtgg ctactagcgc catagagccc accgcatccc
      481 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
      541 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
      601 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
      661 atggctggca actagaaggc acaggtgcaa cgttgttact tgtacagctc gtccatgtca
      721 tgtgtaatcc cggcggcggt caccctctcc ttcaggacca tgtgatcgcg cttctcgttg
      781 gggtctttgc tcagtttgga ctgatggctc aggtagtggt tgtcgggcag cagcacgggg
      841 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc gccgtcctca
      901 acgttgtggc gggtcttgaa gttagccttg atgccgttct tctgcttgtc ggccgtgata
      961 tagaccttgt ggctgttgaa gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     1021 ttgaagtcga tgcccttcag cacgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     1081 tcggcgcggg tcttgtaggt accgtcgtcc ttgaaagaga tggtgcgctc ctggacgtag
     1141 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
     1201 aagcaggcca cgccgtagcc taaggtggtc acgagggtgg gccagggcac gggcagcttg
     1261 ccggtggtgc agatgaactt cagggtcagc ttgccgttgg tggcatcgcc ctcgccctcg
     1321 ccgcggacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     1381 accccggtga acagctcctc gcccttgctc accatggtgg ctcattgcta gcgatgagct
     1441 ctggtagtag actagtggcc cccattatat accctctaga gttgcttgat cagatcaaat
     1501 ccgataactt cgtataatgt atgctatacg aagttatacg cgttcgctcg aattaatcaa
     1561 ttctttgcca aaatgatgag acagcacaat aaccagcacg ttgcccagga gctgtaggag
     1621 gtaccggatc cagtcgactg aattggttcc catggtggag cctgcttttt tgtacaaagt
     1681 cccgggcccg cggtaccgtc gactgcagaa ttcaatttaa atcgtaccga gcgactcgag
     1741 ataacttcgt ataatgtatg ctatacgaag ttattcggat ttgatccaga ggagggcacg
     1801 tcctcactct acacgtcctc actccgcacg tcctcactct acacgtcctc actccgcacg
     1861 tcctcactct acacgtcctc actccaattc agatctcccg atccgagtac gaacatggta
     1921 gctagtggat ccttcgtcga cctgcagact ggctgtgtat aagggagcct gacatttata
     1981 ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag
     2041 ccacttcttc cccgataacg gagaccggca cactggcgca tatcggtggt catcatgcgc
     2101 cagctttcat ccccgatatg caccaccggg taaagttcac gcatatgaag ctggacatca
     2161 cctcccacaa cctcgaggtg gaacagtacg ctgatatctc ttatactgct ccggtgcccg
     2221 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2281 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2341 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2401 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2461 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacgcccct
     2521 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     2581 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cttgggcgct
     2641 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     2701 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     2761 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     2821 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     2881 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     2941 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     3001 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     3061 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     3121 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     3181 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     3241 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     3301 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     3361 gtttttttct tccatttcag gtgtcgtgag agctcgttta gtgaaccgtc agatcgcctg
     3421 gagacgccat ccacgctgta atgagccacc atggcgcgta aggtcgatct cacctcctgc
     3481 gatcgcgagc cgatccacat ccccggcagc attcagccgt gcggctgcct gctagcctgc
     3541 gacgcgcagg cggtgcggat cacgcgcatt acggaaaatg ccggcgcgtt ctttggacgc
     3601 gaaactccgc gggtcggtga gctactcgcc gattacttcg gcgagaccga agcccatgcg
     3661 ctgcgcaacg cactggcgca gtcctccgat ccaaagcgac cggcgctgat cttcggttgg
     3721 cgcgacggcc tgaccggccg caccttcgac atctcactgc atcgccatga cggtacatcg
     3781 atcatcgagt tcgagcctgc ggcggccgaa caggccgaca atccgctgcg gctgacgcgg
     3841 cagatcatcg cgcgcaccaa agaactgaag tcgctcgaag agatggccgc acgggtgccg
     3901 cgctatctgc aggcgatgct cggctatcac cgcgtgatgt tgtaccgctt cgcggacgac
     3961 ggctccggga tggtgatcgg cgaggcgaag cgcagcgacc tcgagagctt tctcggtcag
     4021 cactttccgg cgtcgctggt cccgcagcag gcgcggctac tgtacttgaa gaacgcgatc
     4081 cgcgtggtct cggattcgcg cggcatcagc agccggatcg tgcccgagca cgacgcctcc
     4141 ggcgccgcgc tcgatctgtc gttcgcgcac ctgcgcagca tctcgccctg ccatctcgaa
     4201 tttctgcgga acatgggcgt cagcgcctcg atgtcgctgt cgatcatcat tgacggcacg
     4261 ctatggggat tgatcatctg tcatcattac gagccgcgtg ccgtgccgat ggcgcagcgc
     4321 gtcgcggccg aaatgttcgc cgacttctta tcgctgcact tcaccgccgc ccaccaccaa
     4381 cgctaacaac gccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4441 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4501 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4561 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4621 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4681 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4741 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4801 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4861 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4921 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4981 cccgccgctc gctgatatag ggcccggtac ctttaagacc aatgacttac aaggcagctg
     5041 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac
     5101 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5161 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5221 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5281 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     5341 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     5401 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5461 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     5521 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5581 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5641 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     5701 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     5761 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5821 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5881 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5941 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6001 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6061 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6121 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6181 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6241 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6301 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     6361 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6421 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6481 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6541 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6601 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6661 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6721 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6781 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6841 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6901 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6961 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7021 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7081 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7141 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7201 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7261 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7321 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7381 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7441 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7501 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7561 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7621 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     7681 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7741 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7801 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7861 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7921 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7981 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8041 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8101 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8161 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8221 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8281 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     8341 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     8401 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     8461 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     8521 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     8581 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     8641 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     8701 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     8761 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     8821 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8881 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8941 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     9001 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     9061 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     9121 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     9181 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     9241 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     9301 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     9361 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     9421 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     9481 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     9541 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     9601 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     9661 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     9721 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     9781 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     9841 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     9901 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     9961 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    10021 aattgaagaa tcgcaaaacc agc
//
