LOCUS       LentiX-(loxPcon-       10329 bp DNA     circular SYN 18-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Aug 18, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        42..146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             147..1007
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(365..384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1178..1766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1667..1686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1920..1937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2054..2075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2090..2120
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2128..2144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2133..2155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2152..2168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2152..2168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2187..2207
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2189..2207
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2235..2461
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2462..2642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2689..2814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    3307..3540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4036..4152
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     polyA_signal    complement(4179..4403)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4418..4984)
                     /codon_start=1
                     /locus_tag="hRAS"
                     /label=hRAS
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     CDS             complement(4985..4999)
                     /codon_start=1
                     /label=GGGGS linker
                     /translation="GGGGS"
     CDS             complement(5000..5014)
                     /codon_start=1
                     /label=GGGGS linker
                     /translation="GGGGS"
     CDS             complement(5015..5722)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    5772..5826
                     /label=ZF43/ZF37_hybridpromoter
     promoter        5801..5825
                     /label=YB_TATA minimal promoter
     misc_feature    5849..5882
                     /label=loxP
     protein_bind    complement(5849..5882)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    6035..6053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6071..6089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6107..6125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6143..6161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6179..6197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6215..6233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6251..6269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6602..6813
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    6820..6828
                     /label=Kozak sequence
     CDS             6826..7569
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             7579..7635
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7648..8346
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             8368..8388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    8402..8990
                     /label=WPRE
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
     LTR             9082..9315
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9387..9508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9424..9443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9478..9497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9548..9683
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9610..9629
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9703..9722)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9704..9722)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9732..9749)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     complement(9732..9748)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9741..9763)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(9890..10329,1..16)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9977..9996)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10187..10208
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
       61 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      121 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      181 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      241 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      301 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      361 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      421 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      481 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      541 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      601 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      661 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
      721 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      781 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      841 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      901 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
      961 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1321 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1861 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1921 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1981 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2041 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2101 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2161 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     2221 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     2281 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     2341 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     2401 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     2461 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     2521 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     2581 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     2641 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     2701 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     2761 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     2821 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     2881 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     2941 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     3001 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     3061 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     3121 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     3181 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     3241 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     3301 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     3361 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     3421 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     3481 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     3541 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     3601 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     3661 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     3721 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     3781 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     3841 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     3901 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     3961 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     4021 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     4081 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     4141 attcaaaatt ttatcgtcca gtgtggtggc tactagcgcc atagagccca ccgcatcccc
     4201 agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc
     4261 cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag
     4321 gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga
     4381 tggctggcaa ctagaaggca caggtgcaac gttgttagga gagcacacac ttgcagctca
     4441 tgcagccggg gccactctca tcaggagggt tcagcttccg cagcttgtgc tgccggatct
     4501 cacgcaccaa cgtgtagaag gcatcctcca ctccctgccg ggtcttggcc gaggtctcga
     4561 tgtaggggat gccgtagctt cgggcgaggt cctgagcctg ccgagattcc acagtgcgtg
     4621 cagccaggtc acacttgttc cccaccagca ccatgggcac gtcatccgag tccttcaccc
     4681 gtttgatctg ctccctgtac tggtggatgt cctcaaaaga cttggtgttg ttgatggcaa
     4741 acacacacag gaagccctcc ccggtgcgca tgtactggtc ccgcatggcg ctgtactcct
     4801 cctggccggc ggtatccagg atgtccaaca ggcacgtctc cccatcaatg accacctgct
     4861 tccggtagga atcctctata gtggggtcgt attcgtccac aaaatggttc tggatcagct
     4921 ggatggtcag cgcactcttg cccacaccga cggcgcccac caccaccagc ttgtattcgg
     4981 tcatcgaacc tcctcctccg cttcccccac cacccttgta cagctcgtcc atgccgccgg
     5041 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
     5101 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
     5161 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
     5221 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
     5281 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
     5341 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
     5401 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
     5461 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
     5521 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
     5581 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
     5641 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
     5701 cctcctcgcc cttgctcacc atttttgcca aaatgatgag acagcacaat aaccagcacg
     5761 ttgcccagga ggctagcgat gagctctggt agtagactag tggcccccat tatataccct
     5821 ctagagttgc ttgatcagat caaatccgat aacttcgtat aatgtatgct atacgaagtt
     5881 atacgcgttc gctcgaattt tacgagggta ggaagtggta cggaaagttg gtataagaca
     5941 aaagtgttgt ggaattgctc caggcgatct gacggttcac taaacgagct ctgcttttat
     6001 aggcgcccac cgtacacgcc taaagcttat acgttctcta tcactgatag ggagtaaact
     6061 ggatatacgt tctctatcac tgatagggag taaactgtag atacgttctc tatcactgat
     6121 agggagtaaa ctggtcatac gttctctatc actgataggg agtaaactcc ttatacgttc
     6181 tctatcactg atagggagta aagtctgcat acgttctcta tcactgatag ggagtaaact
     6241 cttcatacgt tctctatcac tgatagggag taaactcgag gaatagtagt accgctagta
     6301 gtacgaacat ggtagctagt ggatccttcg tcgacctgca gactggctgt gtataaggga
     6361 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
     6421 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg cgcatatcgg
     6481 tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgcatat
     6541 gaagctggac atcacctccc acaacctcga ggtggaacag tacgctgata tctcttatac
     6601 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6661 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6721 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6781 ctttttcgca acgggtttgc cgccagaaca cagtaatgtg ccaccatgag ccggctggac
     6841 aagagcaaag tgatcaacag cgccctggaa ctgctgaacg gcgtgggcat cgagggcctg
     6901 accacccgga agctggccca gaaactgggc gtggaacagc ccaccctgta ctggcacgtg
     6961 aagaacaagc gggccctgct ggacgccctg cccatcgaga tgctggaccg gcaccacacc
     7021 cacagctgcc ctctggaagg cgagagctgg caggacttcc tgcggaacaa cgccaagagc
     7081 tacagatgcg ccctgctgag ccaccgggac ggcgccaaag tgcacctggg caccagaccc
     7141 accgagaagc agtacgagac actggaaaac cagctggcct tcctgtgcca gcagggcttc
     7201 agcctggaaa acgccctgta cgccctgagc gccgtgggcc acttcaccct gggctgcgtg
     7261 ctggaagaac aggaacacca ggtcgccaaa gaggaaagag agacacccac caccgacagc
     7321 atgccccccc tgctgaagca ggccatcgag ctgttcgaca gacagggcgc cgagcccgcc
     7381 ttcctgttcg gcctggaact gatcatctgc ggcctcgaga agcagctgaa gtgcgagagc
     7441 ggcggaccca ccgacgccct ggacgacttc gacctggaca tgctgcccgc cgatgccctg
     7501 gatgattttg atctggatat gctccctgct gacgctctcg atgacttcga tctcgatatg
     7561 ctgccaggcg gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag
     7621 gagaaccctg gacctgcacc aggaagtatg agcgagctga ttaaggagaa catgcacatg
     7681 aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa
     7741 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
     7801 cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac
     7861 cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag
     7921 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     7981 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct
     8041 gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac
     8101 ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc
     8161 gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc
     8221 gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga aacgtacgtc
     8281 gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag
     8341 cttaatgagc cagcgaattc ctgcaggccc aagaaaaaac gcaaggtgta gcaacgccga
     8401 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     8461 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     8521 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     8581 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     8641 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8701 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8761 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     8821 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8881 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8941 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc cgctcgctga
     9001 tatagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     9061 ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc
     9121 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9181 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9241 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9301 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     9361 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     9421 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9481 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     9541 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9601 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9661 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     9721 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     9781 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     9841 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     9901 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9961 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    10021 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    10081 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    10141 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    10201 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    10261 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    10321 aacaaaata
//
