LOCUS       plasmid                 7175 bp    DNA     circular SYN 29-FEB-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     rep_origin      complement(2541..2969)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(5395..5774)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    complement(3015..3239)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(5191..5394)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1340..2131)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(1040..1161)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        6040..6144
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6145..7005
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3449..5056)
                     /note="pLannotate"
                     /label="SRC"
                     /database="swissprot"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3295..3336)
                     /note="pLannotate"
                     /label="V5 tag"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(2198..2527)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(5074..5098)
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    3423..3447
                     /note="pLannotate"
                     /label="attB2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(5128..5146)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(3362..3412)
                     /note="pLannotate"
                     /label="3xFLAG (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="65.4"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1156..1314)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="98.1"
                     /match_length="42.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    5929..5981
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     LTR             complement(5162..5195)
                     /note="pLannotate"
                     /label="5' LTR (truncated) (fragment)"
                     /database="snapgene"
                     /identity="97.1"
                     /match_length="18.8"
                     /fragment="True"
                     /other="LTR"
     promoter        complement(2990..3011)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgcg
     1321 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     1381 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     1441 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     1501 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     1561 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
     1621 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     1681 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     1741 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     1801 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     1861 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     1921 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     1981 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     2041 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     2101 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     2161 cagatccgaa aatggatata caagctcccg ggagcttttt gcaaaagcct aggcctccaa
     2221 aaaagcctcc tcactacttc tggaatagct cagaggcaga ggcggcctcg gcctctgcat
     2281 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
     2341 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt
     2401 gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga
     2461 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact
     2521 gacacacatt ccacagaatt aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2581 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2641 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     2701 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     2761 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     2821 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     2881 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     2941 cgcgcttaat gcgccgctac agggcgcgtg gggatacccc ctagagcccc agctggttct
     3001 ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
     3061 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
     3121 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
     3181 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt
     3241 cgaggctgat cagcgggttt aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga
     3301 atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagatc
     3361 acttatcgtc gtcatccttg taatctccct tatcgtcgtc atccttgtaa tcctctagat
     3421 caaccacttt gtacaagaaa gctgggtcga ggttctcccc gggctggtac tggggcgcgg
     3481 tggacgtgaa gtagtcctcc aggaaggcct gcaggtactc gaaggtgggc cgctcctcag
     3541 gctccttccg ccagcactgg cacatgaggt cgtgcaggga ctcgggacac tccggcgggc
     3601 agggcatccg gtagccccgc tccacctggt ccagcacctc gcggttcacc atcccagggt
     3661 agggcacccg tccctttgtg gtgagctcag tcagcaggat cccgaaggac cacacgtccg
     3721 acttgatggt gaagcggcca tagagggcag cttctggagc cgtccacttg atggggaatt
     3781 tggcaccttg ccgcgccgtg tactcattgt cttcaatgag ccgagccagc ccaaagtcgg
     3841 ccactttgca caccaggttc tctcccacca ggatgttggc tgcacgaagg tcccggtgga
     3901 cgtagttcat ccgctccacg tacgccatgc ctgaggcgat ctgagcagcc atgtccacca
     3961 gctgaggcag ccgcaggtac ttgcctgtct cccccttgag aaagtccagc aaactcccct
     4021 tgctcatgta ctccgtgacg atgtaaatgg gctcctctga aaccacagca tacaactgca
     4081 ccagcttctc atgcctcagc ttcttcatga cctgggcctc ctgcaggaag gcctctggag
     4141 acatcgtgcc aggcttcagg gttttgatgg ccaccctggt ggtaccgttc caggtcccca
     4201 tccacacctc gccaaagcag ccctggccca gcttgacctc cagccgcagc gactcccgag
     4261 ggatctccca ggcatccttg gccaggccct gagtctgcgg cttggacgtg gggcacacgg
     4321 tggtgaggcg gtggcacagg ccatcggcgt gtttggagta gtaggccacc agctgctgca
     4381 ggctgttgaa ctgggtgcgg gaggtgatgt agaagccgcc gctgtccagc ttgcggatct
     4441 tgtagtgctt cacgttgagg cccttggcgt tgtcgaagtc agacactgag aggcagtagg
     4501 cacctttcgt ggtctcactt tctcgcacga ggaaggtccc tctcgggttc tctgcattga
     4561 gcagtaaccg ctctgactcc cgtctggtga tcttgccaaa ataccactcc tcagcctgga
     4621 tggagtcgga gggcgccacg tagttgctgg ggatgtagcc tgtctgtcct gtgctgagcg
     4681 agtgggccag ccaccagtct ccctctgtgt tgttgacaat ctggagccgc tcgcctttct
     4741 tgaaggacag gtctgtctcc gtcctagact catagtcata gagggccaca aaggtggtca
     4801 ctccaccggc cagcgggccc gccctctgcg gggaggtgac ggtgtccgag gagttgaagc
     4861 ctccgaacag cttgggctcg gcggccgcgg gggcgaaggc cgcgctgggg ccgcggtggc
     4921 cgtcggccga ggctggcttg ctgggggtct gcgaggcggg gaaagcgccc ccgccagcgc
     4981 cgtgcacgtt ctcggcgggc tccaggctgc ggcgccgctg gctggcatcc ttgggcttgc
     5041 tcttgttgct acccatggtt ctatctcctt cgaagcctgc ttttttgtac aaacttgttg
     5101 atagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt cgataagcca
     5161 gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct cccaccgtac
     5221 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
     5281 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
     5341 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
     5401 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
     5461 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
     5521 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
     5581 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
     5641 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
     5701 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
     5761 caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
     5821 aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat
     5881 tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa
     5941 ctatgcggca tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc
     6001 gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6061 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6121 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6181 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6241 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6301 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6361 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6421 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6481 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6541 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6601 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6661 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6721 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6781 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6841 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6901 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6961 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7021 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7141 gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
